Functional Aspects of Three Modified Nucleosides, Ψ, ms2io6A, and m1G, Present in the Anticodon Loop of tRNA

1993 ◽  
pp. 67-78 ◽  
Author(s):  
Tord G. Hagervall ◽  
Birgitta Esberg ◽  
Ji-nong Li ◽  
Thérèse M. F. Tuohy ◽  
John F. Atkins ◽  
...  
Keyword(s):  
Modified Nucleosides ◽  
Anticodon Loop ◽  
Functional Aspects

Structural significance of modified nucleosides k2C and t6A present in the anticodon loop of tRNAIle

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 14176 ◽  
Author(s):  
Susmit B. Sambhare ◽  
Bajarang V. Kumbhar ◽  
Asmita D. Kamble ◽  
Rohit S. Bavi ◽  
Navanath M. Kumbhar ◽  
...  
Keyword(s):  
Modified Nucleosides ◽  
Anticodon Loop ◽  
Structural Significance

Quantitative aspects of metal ion binding to certain transfer RNA anticodon loop modified nucleosides

1984 ◽  
Vol 802 (2) ◽  
pp. 352-361 ◽  
Author(s):  
M SCHWEIZER ◽  
N DE ◽  
M PULSIPHER ◽  
M BROWN ◽  
P REDDY ◽  
...  
Keyword(s):  
Metal Ion ◽  
Transfer Rna ◽  
Modified Nucleosides ◽  
Ion Binding ◽  
Anticodon Loop ◽  
Metal Ion Binding

Methylation of tRNAAsp by the DNA Methyltransferase Homolog Dnmt2

Science ◽  
2006 ◽  
Vol 311 (5759) ◽  
pp. 395-398 ◽  
Author(s):  
Mary Grace Goll ◽  
Finn Kirpekar ◽  
Keith A. Maggert ◽  
Jeffrey A. Yoder ◽  
Chih-Lin Hsieh ◽  
...  
Keyword(s):  
Small Rna ◽  
Dna Methyltransferase ◽  
Dna Methyltransferases ◽  
Transfer Rna ◽  
Modified Nucleosides ◽  
Anticodon Loop ◽  
Sequence Recognition ◽  
Eukaryotic Dna ◽  
Biochemical Approach

The sequence and the structure of DNA methyltransferase-2 (Dnmt2) bear close affinities to authentic DNA cytosine methyltransferases. A combined genetic and biochemical approach revealed that human DNMT2 did not methylate DNA but instead methylated a small RNA; mass spectrometry showed that this RNA is aspartic acid transfer RNA (tRNAAsp) and that DNMT2 specifically methylated cytosine 38 in the anticodon loop. The function of DNMT2 is highly conserved, and human DNMT2 protein restored methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that was dependent on preexisting patterns of modified nucleosides. Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase.

Undecagold labeling of tRNA

1991 ◽  
Vol 49 ◽  
pp. 44-45
Author(s):  
James F. Hainfeld ◽  
Kyra M. Alford ◽  
Mathias Sprinzl ◽  
Valsan Mandiyan ◽  
Santa J. Tumminia ◽  
...  
Keyword(s):  
High Resolution ◽  
Transmission Electron Micrograph ◽  
Anticodon Loop ◽  
Electron Micrograph ◽  
Reaction Conditions ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission

The undecagold (Au11) cluster was used to covalently label tRNA molecules at two specific ribonucleotides, one at position 75, and one at position 32 near the anticodon loop. Two different Au11 derivatives were used, one with a monomaleimide and one with a monoiodacetamide to effect efficient reactions.The first tRNA labeled was yeast tRNAphe which had a 2-thiocytidine (s2C) enzymatically introduced at position 75. This was found to react with the iodoacetamide-Aun derivative (Fig. 1) but not the maleimide-Aun (Fig. 2). Reaction conditions were 37° for 16 hours. Addition of dimethylformamide (DMF) up to 70% made no improvement in the labeling yield. A high resolution scanning transmission electron micrograph (STEM) taken using the darkfield elastically scattered electrons is shown in Fig. 3.

Functional Aspects of Language Acquisition: Another View

1978 ◽  
Vol 23 (9) ◽  
pp. 623-624
Author(s):  
MARIS MONITZ RODGON
Keyword(s):  
Language Acquisition ◽  
Functional Aspects

МАРКЕТИНГОВІ АСПЕКТИ ФУНКЦІОНУВАННЯ ЛІТЕРАТУРНИХ КОНКУРСІВ ТА ПРЕМІЙ

2020 ◽  
pp. 90
Author(s):  
O. Bondar
Keyword(s):  
Publishing House ◽  
Target Audience ◽  
Point Of View ◽  
Information Support ◽  
Literary Field ◽  
Non Profit ◽  
Functional Aspects ◽  
The Media ◽  
Literary Prizes ◽  
The Impact

<p><em>In this study, I have collected and summarized the functional aspects of a literary prize, contest, and rating, which indicate their affiliation with the marketing complex of the publishing house for the first time. For this purpose, I have analyzed and summarized the common concepts of the functioning of literary prizes and contests as advertising tools for publishing activity. Because the previous studies are only focused on the fact of the impact of the prize on the promotion of editions but do not explain it, these aspects have been considered and introduced by me from the book production’s point of view. I investigated that the prizes and the contests in the literary field are effective marketing tools, which meet many publisher’s needs at the same time and can be considered a non-profit form of capital. I have reviewed the works of other authors, who accept that the economic success of the book is rising if the author is a winner of the literary prize or contest. I have found out that the book prize activates the demand for the book, and the literary contest is a tool to track the reader’s reaction to a future publication. In this way, literary prizes and contests can be considered as a way of conducting a marketing dialogue with the target audience. I have focused on the information support of literary national and international prizes and contests by the media, which attracts attention to the book and forms the reader’s interest. The literary prizes and contests are also considered as a way of exploring trends and their changes, familiarization the popular genres among the target audience and fixation the current choice of modern readers. Literary prizes and contests motivate the authors to improve their literary excellence, are the source of new authors and works, and assist in increasing sales of books. However, further research is recommended.</em></p><strong><em>Key words:</em></strong><em> book prize, book rating, literary contest, literary prize, functions of the literary prizes.</em>

Pathological verbal repetition by Chinese elders with Dementia of Alzheimer’s Type

2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Lin Zhu ◽  
Lihe Huang
Keyword(s):  
Cognitive Impairment ◽  
Nursing Staff ◽  
Systemic Functional Linguistics ◽  
Effective Communication ◽  
Linguistic Markers ◽  
Word Repetition ◽  
Functional Aspects ◽  
Early Dementia ◽  
Dementia Of Alzheimer’S Type ◽  
Verbal Repetition

Verbal repetition has been acknowledged as one of the most common symptoms in early Dementia of Alzheimer's Type (DAT). Despite previous attempts, the applicability of verbal repetition as an essential linguistic marker indicating this disease remains unexplored for Chinese DAT patients. This study collects Chinese DAT patients' daily conversation data to investigate both structural and functional aspects of pathological verbal repetition. Three major types are set regarding the cases of pathological repetition, respectively ‘unconscious concept repetition', ‘perseveration', and ‘involuntary word repetition'. The analysis focuses on what features these repetitions have from the perspective of ‘ideational function', ‘interpersonal function', and ‘textual function' within the framework of Systemic Functional Linguistics. A better understanding of pathological verbal repetition by DAT elders as the linguistic markers of cognitive impairment promotes effective communication between patients, nursing staff, and family members.

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