Regulation of 5-Lipoxygenase mRNA Expression in the Human Monocytic Cell Line Mono Mac 6 by Transforming Growth Factor-ß and 1,25-Dihydroxyvitamin D3

1999 ◽  
pp. 105-111 ◽  
Author(s):  
Damaris Härle ◽  
Olof Rådmark ◽  
Bengt Samuelsson ◽  
Dieter Steinhilber
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell

scholarly journals Differentiation of a human monocytic cell line by 1,25-dihydroxyvitamin D3 (calcitriol): a morphologic, phenotypic, and functional analysis

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1110-1115 ◽  
Author(s):  
WF Rigby ◽  
L Shen ◽  
ED Ball ◽  
PM Guyre ◽  
MW Fanger
Keyword(s):  
Cell Line ◽  
De Novo ◽  
Specific Antigen ◽  
Myeloid Differentiation ◽  
Macrophage Phenotype ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Monocytic Cell Line

Abstract Several recent studies have suggested a role for 1,25-dihydroxyvitamin D3 (calcitriol) in myeloid differentiation. We have examined the effects of calcitriol on the U937 monoblast cell line and found that calcitriol, at near physiologic concentrations, is a potent inhibitor of U937 growth. Moreover, calcitriol induces differentiation to a monocyte-macrophage phenotype marked by enhanced alpha-naphthyl esterase staining. Morphologic changes were attended by de novo induction of the myeloid specific antigen detected by the monoclonal antibody AML-2–23, as well as dramatic increases in Fc receptors for IgG. In addition, calcitriol induced U937 cells to perform phagocytosis and antibody-dependent cellular cytotoxicity. These results indicate the potential activity of calcitriol in myeloid differentiation and additionally suggest a role for calcitriol in monocyte-macrophage activation.

scholarly journals Differentiation of a human monocytic cell line by 1,25-dihydroxyvitamin D3 (calcitriol): a morphologic, phenotypic, and functional analysis

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1110-1115 ◽  
Author(s):  
WF Rigby ◽  
L Shen ◽  
ED Ball ◽  
PM Guyre ◽  
MW Fanger
Keyword(s):  
Cell Line ◽  
De Novo ◽  
Specific Antigen ◽  
Myeloid Differentiation ◽  
Macrophage Phenotype ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Monocytic Cell Line

Several recent studies have suggested a role for 1,25-dihydroxyvitamin D3 (calcitriol) in myeloid differentiation. We have examined the effects of calcitriol on the U937 monoblast cell line and found that calcitriol, at near physiologic concentrations, is a potent inhibitor of U937 growth. Moreover, calcitriol induces differentiation to a monocyte-macrophage phenotype marked by enhanced alpha-naphthyl esterase staining. Morphologic changes were attended by de novo induction of the myeloid specific antigen detected by the monoclonal antibody AML-2–23, as well as dramatic increases in Fc receptors for IgG. In addition, calcitriol induced U937 cells to perform phagocytosis and antibody-dependent cellular cytotoxicity. These results indicate the potential activity of calcitriol in myeloid differentiation and additionally suggest a role for calcitriol in monocyte-macrophage activation.

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