The Role of Glycosylation in Flavonol-Induced Pollen Germination

1998 ◽  
pp. 35-44 ◽  
Author(s):  
Loverine P. Taylor ◽  
Darren Strenge ◽  
Keith D. Miller
Keyword(s):  
Pollen Germination

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

The role of reactive oxygen species in pollen germination in Picea pungens (blue spruce)

2018 ◽  
Vol 31 (4) ◽  
pp. 357-365 ◽  
Author(s):  
Nikita Maksimov ◽  
Anastasia Evmenyeva ◽  
Maria Breygina ◽  
Igor Yermakov
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Germination ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Blue Spruce ◽  
Picea Pungens

scholarly journals FLORAL-DERIVED VOLATILE COMPOUNDS: A ROLE IN POLLINATION?

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1128d-1128
Author(s):  
Douglas D. Archbold ◽  
Thomas R. Hamilton-Kemp ◽  
John H. Loughrin
Keyword(s):  
Volatile Compounds ◽  
Pollen Germination ◽  
Potential Role ◽  
Apple Fruit ◽  
Capillary Gc ◽  
Full Bloom ◽  
Percent Germination

Volatile compounds, commonly produced by flowers during bloom, have been described as insect attractants. Some of these compounds stimulate Pinus pollen germination in vitro (French et al., 1979, J. Agric. Fd. Chem., 27184-187), suggesting that such compounds may do the same in vivo. Red Delicious apple pollen was germinated on agar in a simple, enclosed in vitro bioassay system in the presence of a number of plant tissues, including apple, tomato, and chrysanthemum leaves, apple flowers, rose petals, and apple fruit slices. These tissues represent a diversity of types of volatile compounds, Pollen germination was recorded by microphotography after 1 and 2 hours, and percent germination was determined. Although stimulation of germination was not observed, macerated tomato leaves inhibited it. To determine if the volatile characteristics of cultivars differ, flowers of Red Delicious and Golden Delicious apple were harvested at full bloom. Their volatiles were collected, identified, and quantitated by capillary GC-MS. Among 8 major compounds common in the two cultivars, several quantitative differences were observed. These results will be discussed in relation to the potential role of volatiles in pollen germination.

scholarly journals A Role of Arabidopsis Inositol Polyphosphate Kinase, AtIPK2α, in Pollen Germination and Root Growth

2004 ◽  
Vol 137 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Jun Xu ◽  
Charles A. Brearley ◽  
Wen-Hui Lin ◽  
Yuan Wang ◽  
Rui Ye ◽  
...  
Keyword(s):  
Root Growth ◽  
Pollen Germination ◽  
Polyphosphate Kinase ◽  
Inositol Polyphosphate

scholarly journals Dynamics of Pollen Activation and the Role of H+-ATPase in Pollen Germination in Blue Spruce (Picea pungens)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1760
Author(s):  
Maria Breygina ◽  
Ekaterina Klimenko ◽  
Alexandra Podolyan ◽  
Alexander Voronkov
Keyword(s):  
Pollen Germination ◽  
Physiological State ◽  
Pollen Tubes ◽  
Ph Gradient ◽  
Atpase Inhibitor ◽  
Picea Pungens ◽  
Specialized Structure ◽  
Angiosperm Pollen ◽  
Pollen Activation

Pollen is a highly specialized structure for sexual plant reproduction. Early stages of pollen germination require the transition from dormant state to active metabolism. In particular, an important role during this early phase of angiosperm pollen germination is played by H+-ATPase. Very little is known about pollen activation in gymnosperm species, and information on the involvement of H+-ATPase is lacking. We tracked four indicators characterizing the physiological state of pollen: membrane potential, intracellular pH, anion efflux and oxygen uptake, in order to monitor the dynamics of activation in Picea pungens. Based on pH dynamics during activation, we assumed the important role of H+-ATPase in spruce pollen germination. Indeed, germination was severely suppressed by P-type ATPase inhibitor orthovanadate. In spruce pollen tubes, a pronounced pH gradient with a maximum in the apical zone was found, which was different from the pollen tubes of flowering plants. Using orthovanadate and fusicoccin, we found that the proton pump is largely responsible for maintaining the gradient. Immunolocalization of the enzyme in pollen tubes showed that the distribution of H+-ATPase generally coincides with the shape of the pH gradient: its maximum accumulation is observed in the apical zone.

scholarly journals Genome wide transcriptome analysis reveals vital role of heat responsive genes in regulatory mechanisms of lentil (Lens culinaris Medikus)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dharmendra Singh ◽  
Chandan Kumar Singh ◽  
Jyoti Taunk ◽  
Vasudha Jadon ◽  
Madan Pal ◽  
...  
Keyword(s):  
Heat Stress ◽  
Transcriptome Analysis ◽  
Pollen Germination ◽  
De Novo ◽  
Nucleotide Polymorphisms ◽  
Sensitive Genotype ◽  
Tolerant Genotype

Abstract The present study reports the role of morphological, physiological and reproductive attributes viz. membrane stability index (MSI), osmolytes accumulations, antioxidants activities and pollen germination for heat stress tolerance in contrasting genotypes. Heat stress increased proline and glycine betaine (GPX) contents, induced superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione peroxidase (GPX) activities and resulted in higher MSI in PDL-2 (tolerant) compared to JL-3 (sensitive). In vitro pollen germination of tolerant genotype was higher than sensitive one under heat stress. In vivo stressed pollens of tolerant genotype germinated well on stressed stigma of sensitive genotype, while stressed pollens of sensitive genotype did not germinate on stressed stigma of tolerant genotype. De novo transcriptome analysis of both the genotypes showed that number of contigs ranged from 90,267 to 104,424 for all the samples with N50 ranging from 1,755 to 1,844 bp under heat stress and control conditions. Based on assembled unigenes, 194,178 high-quality Single Nucleotide Polymorphisms (SNPs), 141,050 microsatellites and 7,388 Insertion-deletions (Indels) were detected. Expression of 10 genes was evaluated using quantitative Real Time Polymerase Chain Reaction (RT-qPCR). Comparison of differentially expressed genes (DEGs) under different combinations of heat stress has led to the identification of candidate DEGs and pathways. Changes in expression of physiological and pollen phenotyping related genes were also reaffirmed through transcriptome data. Cell wall and secondary metabolite pathways are found to be majorly affected under heat stress. The findings need further analysis to determine genetic mechanism involved in heat tolerance of lentil.

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