Kidney Cell Cultures in Hormonally Defined Serum-Free Medium

1984 ◽  
pp. 129-150 ◽  
Author(s):  
Mary Taub
Keyword(s):  
Cell Cultures ◽  
Kidney Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins

2019 ◽  
Vol 71 (5) ◽  
pp. 949-962 ◽  
Author(s):  
Sandra Fernanda Suárez-Patiño ◽  
Thaissa Consoni Bernardino ◽  
Eutimio Gustavo Fernández Núñez ◽  
Renato Mancini Astray ◽  
Carlos Augusto Pereira ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Semliki Forest Virus ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Effects of vitamin A on growth and differentiation of human tracheobronchial epithelial cell cultures in serum-free medium

1989 ◽  
Vol 93 (1) ◽  
pp. 133-142
Author(s):  
D.P. Chopra ◽  
M.M. Klinger ◽  
J.K. Sullivan
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Vitamin A ◽  
Cell Cultures ◽  
Cell Multiplication ◽  
Serum Free Medium ◽  
Free Medium ◽  
Plasma Membrane Vesicles ◽  
Serum Free ◽  
Epithelial Cell Cultures

Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(−6) M and 10(−7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.

Peptides extracted from vero cell cultures overcome the blastocyst block of mouse embryos in a serum-free medium

1994 ◽  
Vol 11 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Hsin-Fu Chen ◽  
Hong-Nerng Ho ◽  
Shee-Uan Chen ◽  
Kuang-Han Chao ◽  
Heng-Ru Lin ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Mouse Embryos ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

A serum-free medium that supports the growth of piscine cell cultures

In Vitro ◽  
1983 ◽  
Vol 19 (11) ◽  
pp. 818-824 ◽  
Author(s):  
Thomas B. Shea ◽  
Eugene S. Berry
Keyword(s):  
Cell Cultures ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Production of somatomedin-like activity by human adult tumor-derived, transformed, and normal cell cultures and by cultured rat hepatocytes: effects of culture conditions and of epidermal growth factor (urogastrone)

1984 ◽  
Vol 62 (12) ◽  
pp. 1343-1350 ◽  
Author(s):  
Paul R. Atkison ◽  
L. Joseph Hayden ◽  
R. Marvin Bala ◽  
Morley D. Hollenberg
Keyword(s):  
Human Skin ◽  
Cell Cultures ◽  
Culture Medium ◽  
Rat Hepatocytes ◽  
Serum Free Medium ◽  
Free Medium ◽  
Kb Cells ◽  
Serum Free ◽  
Epidermal Growth ◽  
Adult Human Skin

We have measured the production of a basic-somatomedin-like activity (SLA) by a variety of human tumor-derived, transformed, and normal postnatal cell cultures; and we have compared the production of SLA by these cell types with the production of SLA by adult rat hepatocytes cultured in serum-free medium. Cells derived from a human epidermoid carcinoma (KB), a pancreatic carcinoma (Panc-1), a Simian virus 40 transformed adult human skin-derived cell line (SV40 fibroblasts), and a normal adult human skin-derived fibroblast line released SLA when cultured in a serum-free growth medium. No SLA was recovered from the culture medium of human choriocarcinoma-derived cells (BeWo) or of a human lymphoblastoid cell line (IM-9). The production of SLA by rat hepatocytes cultured in serum-free medium appeared to exceed the production of SLA by the other cell cultures. In cultures of KB cells, SV40 fibroblasts, and rat hepatocytes, the production of SLA depended on the frequency with which the growth medium was renewed; in general, the highest rates of SLA production were observed when the medium was renewed every 48–72 h. The presence of mouse epidermal growth factor (urogastrone) (EGF-URO) in the serum-free culture medium stimulated the production of SLA by KB cells and by rat hepatocytes, but did not increase SLA production by normal or by SV40-transfonned human skin-derived fibroblasts. We conclude that tumor-derived cells are capable of producing somatomedin-like activity and that the production of SLA by such cells can be subject to controls (nutrient availability, EGF-URO stimulation) that regulate SLA production, either by normal adult tissues, like liver, or by a variety of normal embryonic tissues.

Quantitative modelling of growth and metabolism of CHO-K1 cell cultures in a serum free medium

2001 ◽  
Author(s):  
U. Nasution ◽  
F. Verhoeye ◽  
G. Bastin ◽  
S. Agathos ◽  
Y-J. Schneider
Keyword(s):  
Cell Cultures ◽  
Serum Free Medium ◽  
Free Medium ◽  
Quantitative Modelling ◽  
Serum Free
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