scholarly journals Insulin and insulin mRNA are detected in neuronal cell cultures maintained in an insulin-free/serum-free medium.

1990 ◽  
Vol 38 (6) ◽  
pp. 829-836 ◽  
Author(s):  
R Schechter ◽  
H F Sadiq ◽  
S U Devaskar
Keyword(s):  
Cell Cultures ◽  
Neuronal Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Neuronal Cell Cultures ◽  
Insulin Mrna ◽  
Free Serum

scholarly journals Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins

2019 ◽  
Vol 71 (5) ◽  
pp. 949-962 ◽  
Author(s):  
Sandra Fernanda Suárez-Patiño ◽  
Thaissa Consoni Bernardino ◽  
Eutimio Gustavo Fernández Núñez ◽  
Renato Mancini Astray ◽  
Carlos Augusto Pereira ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Semliki Forest Virus ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Effects of vitamin A on growth and differentiation of human tracheobronchial epithelial cell cultures in serum-free medium

1989 ◽  
Vol 93 (1) ◽  
pp. 133-142
Author(s):  
D.P. Chopra ◽  
M.M. Klinger ◽  
J.K. Sullivan
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Vitamin A ◽  
Cell Cultures ◽  
Cell Multiplication ◽  
Serum Free Medium ◽  
Free Medium ◽  
Plasma Membrane Vesicles ◽  
Serum Free ◽  
Epithelial Cell Cultures

Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(−6) M and 10(−7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.

Peptides extracted from vero cell cultures overcome the blastocyst block of mouse embryos in a serum-free medium

1994 ◽  
Vol 11 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Hsin-Fu Chen ◽  
Hong-Nerng Ho ◽  
Shee-Uan Chen ◽  
Kuang-Han Chao ◽  
Heng-Ru Lin ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Mouse Embryos ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

A multi-omics approach to metabolically optimize a xeno free, serum free medium for T cell expansion

Cytotherapy ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. S28
Author(s):  
A. Pierce ◽  
S. Mansour ◽  
N. Kamath ◽  
P. Lin
Keyword(s):  
T Cell ◽  
Cell Expansion ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
T Cell Expansion ◽  
Free Serum

scholarly journals Electrohydrodynamic jetting of mouse neuronal cells

2006 ◽  
Vol 394 (2) ◽  
pp. 375-378 ◽  
Author(s):  
Peter A. M. Eagles ◽  
Amer N. Qureshi ◽  
Suwan N. Jayasinghe
Keyword(s):  
Field Strength ◽  
Applied Voltage ◽  
Neuronal Cells ◽  
Neuronal Cell ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Differentiated Cells ◽  
Novel Approach ◽  
Control Samples

CAD (Cath.a-differentiated) cells, a mouse neuronal cell line, were subjected to electrohydrodynamic jetting at a field strength of 0.47–0.67 kV/mm, corresponding to an applied voltage of 7–10 kV. After jetting, the cells appeared normal and continued to divide at rates similar to those shown by control samples. Jetted cells, when placed in serum-free medium, underwent differentiation that was sustained for at least 1 month. Some of the droplets produced by jetting contained only a few cells. These results indicate that the process of jetting does not significantly perturb neuronal cells and that this novel approach might in the future be a useful way to deposit small numbers of living nerve cells on to surfaces.

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