Peptides extracted from vero cell cultures overcome the blastocyst block of mouse embryos in a serum-free medium

Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(−6) M and 10(−7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.

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Rat liver epithelial cell cultures in a serum-free medium: Primary cultures and derived cell lines expressing differentiated functions

In Vitro ◽

10.1007/bf02618294 ◽

1984 ◽

Vol 20 (10) ◽

pp. 780-795 ◽

Cited By ~ 37

Author(s):

Martine Chessebeuf ◽

Prudent Padieu

Keyword(s):

Epithelial Cell ◽

Cell Lines ◽

Rat Liver ◽

Cell Cultures ◽

Primary Cultures ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Epithelial Cell Cultures

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Formulation of a complex serum-free medium (CSM) for use in the co-culture of mouse embryos with cells of the female reproductive tract

Reproduction Fertility and Development ◽

10.1071/rd9910099 ◽

1991 ◽

Vol 3 (1) ◽

pp. 99 ◽

Cited By ~ 6

Author(s):

D Sakkas ◽

AO Trounson

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Embryo Culture ◽

Reproductive Tract ◽

Culture Media ◽

Mouse Embryos ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Cell Numbers

Co-culture of pre-implantation embryos with cells of the reproductive tract requires a medium that is beneficial to both embryos and cells. However, many studies in this area utilize media originally formulated for specific cell lines. In the present study, a complex serum-free medium (CSM) was formulated on the basis of the ionic compositions of existing embryo culture media and mouse oviductal fluid as well as the concentrations of growth factors that appear to benefit mouse embryo development. The study began by investigating the effect of altering the concentrations of K+ ions (0-40 mM) and sulfate ions (0-10 mM) in embryo culture media on the development of 2-cell mouse embryos. Mouse embryos showed improved cell numbers at the blastocyst stage when cultured in 10 mM K+ compared with Whittingham's T6 medium. Embryos were also cultured in T6 supplemented with bovine serum albumin (BSA) containing various concentrations of insulin, insulin-like growth factors I and II, fibroblast growth factor, and epidermal growth factor. Insulin concentrations of 100 ng mL-1 significantly (P less than 0.05) improved the cell numbers of 2-cell embryos cultured to the morulae and blastocyst stages compared with those cultured in T6 + BSA alone. CSM was formulated on the basis of the results of these experiments and was found to support both improved development of 2-cell mouse embryos and the culture of mouse fibroblast and mouse oviduct cells.

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