Use of Thin-Layer Chromatography, High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry to Investigate the Relationship Between Juvenile Hormone Titre and Corpus Allatum Activity in Adult Male Periplaneta Americana

1990 ◽  
pp. 9-18 ◽  
Author(s):  
R. J. Weaver ◽  
J. P. G. Wilkins ◽  
A. T. Roberts ◽  
J. P. Edwards
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Periplaneta Americana ◽  
Corpus Allatum ◽  
Gas Chromatography Mass Spectrometry ◽  
Corpus Allatum Activity ◽  
The Relationship ◽  
Layer Chromatography

Separation and Identification of Phencyclidine and Some of Its Analogs

1983 ◽  
Vol 66 (5) ◽  
pp. 1186-1195
Author(s):  
K Gurudath Rao ◽  
Shiv K Soni
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mass Spectra ◽  
Major Ions ◽  
Gas Chromatography Mass Spectrometry ◽  
Structural Variations ◽  
Layer Chromatography

Abstract l-(l-Phenylcyclohexyl)piperidine (PCP) and 9 of its analogs have been separated and identified by using thin layer chromatography, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and infrared spectroscopy (IR). Spectral and chromatographic characteristics are discussed in terms of the structural variations observed in these compounds. Some compounds decomposed on the GC column; the HPLC procedure is a helpful substitute. A tentative scheme is proposed for the fragmentation of the major ions observed in the mass spectra. The effect of substitution on the IR spectra of the analogs was investigated.

Tetrodotoxin in Gastropods (Snails) Implicated in Food Poisoning in Northern Taiwan

2002 ◽  
Vol 65 (8) ◽  
pp. 1341-1344 ◽  
Author(s):  
DENG-FWU HWANG ◽  
YU-CHIUNG SHIU ◽  
PAI-AN HWANG ◽  
YA-HUI LU
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Food Poisoning ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellulose Acetate Membrane ◽  
Layer Chromatography ◽  
Northern Taiwan

The toxin in the gastropods (snails) Zeuxis sufflatus and Niotha clathrata implicated in a food poisoning incident in northern Taiwan in April 2001 was studied. The symptoms exhibited by four victims were general paresthesia, paralysis of the phalanges and the extremities, paralysis, coma, vomiting, and aphasia. The remaining gastropods were assayed for toxicity in the form of tetrodotoxin (TTX). The ranges of specimen toxicity were 345 to 1,640 mouse units (MU) for Z. sufflatus and 190 to 643 MU for N. clathrata. The toxicities of the digestive gland and for other parts of the gastropod were 1,120 ± 477 MU and 497 ± 238 MU, respectively, for Z. sufflatus and 683 ± 113 MU and 289 ± 169 MU, respectively, for N. clathrata. The toxin from the methanolic extract of the gastropods was partially purified by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, thin-layer chromatography, high-performance liquid chromatography, and gas chromatography–mass spectrometry analyses demonstrated that the toxin consisted of TTX. It was concluded that the causative agent of the food poisoning in question was TTX.

scholarly journals A Tetrodotoxin-Producing Vibrio Strain, LM-1, from the Puffer Fish Fugu vermicularis radiatus

2000 ◽  
Vol 66 (4) ◽  
pp. 1698-1701 ◽  
Author(s):  
Myoung-Ja Lee ◽  
Dong-Youn Jeong ◽  
Woo-Seong Kim ◽  
Hyun-Dae Kim ◽  
Cheorl-Ho Kim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Mouse Bioassay ◽  
Puffer Fish ◽  
Layer Chromatography

ABSTRACT Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fishFugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX, 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fish.

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