Formation of Superoxide Anions and Hydrogen Peroxide by Polymorphonuclear Leukocytes Stimulated with Cytochalasin

1982 ◽  
pp. 361-370
Author(s):  
Shigeki Minakami ◽  
Zeenat F. Nabi ◽  
Bernard Tatscheck ◽  
Koichiro Takeshige
Keyword(s):  
Hydrogen Peroxide ◽  
Polymorphonuclear Leukocytes ◽  
Superoxide Anions

TRK-530 Inhibits Accumulation of Superoxide Anions Derived from Human Polymorphonuclear Leukocytes and Bone Resorption Induced by Activated Osteoclasts

Pharmacology ◽  
1998 ◽  
Vol 56 (3) ◽  
pp. 125-130 ◽  
Author(s):  
Masahiko Tanahashi ◽  
Yuriko Funaba ◽  
Akihiro Tateishi ◽  
Norio Kawabe ◽  
Teruo Nakadate-Matsushita
Keyword(s):  
Bone Resorption ◽  
Polymorphonuclear Leukocytes ◽  
Superoxide Anions ◽  
Human Polymorphonuclear Leukocytes

BACTERICIDAL FUNCTION OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES

PEDIATRICS ◽  
1972 ◽  
Vol 50 (2) ◽  
pp. 264-270
Author(s):  
Paul G. Quie
Keyword(s):  
Hydrogen Peroxide ◽  
Chronic Granulomatous Disease ◽  
Polymorphonuclear Leukocytes ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Granulomatous Disease ◽  
Serum Factors ◽  
Gram Negative ◽  
Increased Risk ◽  
Human Polymorphonuclear Leukocytes

Serum from most normal persons contains specific antibodies which react with common bacterial species preparing their surfaces so that phagocytosis by leukocytes can take place. The Fab part of these antibodies reacts with immunologic specificity with antigens on the surface of bacteria. Another part of the immunoglobulin molecule termed the Fc portion is activated during the attachment of the Fab portion to bacteria and becomes a site for attachment of bacteria to receptors on the surface of phagocytic cells. This activity is greatly amplified by heat-labile serum factors. Normally bacteria are rapidly killed by human polymorphonuclear leukocytes after engulfment occurs. However staphylococci and gram-negative species of bacteria survive in the leukocytes of patients with the syndrome "Chronic Granulomatous Disease of Childhood." These patients have suffered recurrent severe infections with bacterial species that are part of the body's resident bacterial flora. By contrast these patients are not at increased risk to infection from such pyogenic bacterial species as group A streptococci or pneumococci. The leukocytes from patients with chronic granulomatous disease produce little hydrogen peroxide during phagocytosis. Catalase-producing staphylococci and gram-negative bacteria are not killed, but hydrogen peroxide-producing streptococci and pneumococci are killed. A normal metabolic response to phagocytosis as well as release of lysosonial factors are essential for the bactericidal activity of human polymorphonuclear leukocytes.

Source of hydrogen peroxide and of chemiluminescence observed in activated human platelet preparations

Blood ◽  
1977 ◽  
Vol 50 (4) ◽  
pp. 597-602 ◽  
Author(s):  
NI Krinsky ◽  
KL Scoon ◽  
JC Hardin ◽  
PH Levine
Keyword(s):  
Hydrogen Peroxide ◽  
Polymorphonuclear Leukocytes ◽  
Human Platelet ◽  
H2o2 Production ◽  
Activated Platelets ◽  
Latex Spheres

Abstract Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 X 10(5) cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phagocytosing polymorphonuclear leukocytes (PMN) and analysis of the purified platelets indicates contamination by PMN at the level of 0.2%. The amount of H2O2 produced and the chemiluminescence observed can be duplicated by adding latex spheres to a preparation of PMN at a concentration equivalent to the contaminant in the platelet preparations. We conclude that the H2O2 produced and chemiluminescence observed from activated platelets is due to the presence of small amounts of contaminating PMN. These studies emphasize the importance of controlling for PMN contamination in studies of platelet biochemistry and physiology.

Effect of Haemophilus somnus on phagocytosis and hydrogen peroxide production by bovine polymorphonuclear leukocytes

1992 ◽  
Vol 13 (3) ◽  
pp. 191-202 ◽  
Author(s):  
Cheryl G. Pfeifer ◽  
Manuel Campos ◽  
Terry Beskorwayne ◽  
Lorne A. Babiuk ◽  
Andrew A. Potter
Keyword(s):  
Hydrogen Peroxide ◽  
Polymorphonuclear Leukocytes ◽  
Hydrogen Peroxide Production ◽  
Haemophilus Somnus

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444 ◽  
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

Production de radicaux superoxydes par radiolyse pulsée de l'eau à transfert d'énergie linéique (TEL) élevé

2001 ◽  
Vol 79 (2) ◽  
pp. 180-183 ◽  
Author(s):  
G Baldacchino ◽  
V Trupin-Wasselin ◽  
S Bouffard ◽  
E Balanzat ◽  
M Gardès-Albert ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Heavy Ions ◽  
Pulse Radiolysis ◽  
Direct Methods ◽  
Superoxide Anions ◽  
Radical Species ◽  
High Let ◽  
High Linear Energy Transfer

The radiolysis of water with heavy ions of high linear energy transfer (LET) (-dE/dx) is characterized, in deaerated medium, by the production of superoxide anions, the radiolytic yields of which increase with the LET. Radiobiological interest in such radical species comes from the oxidative stress which may be generated by their dismutation in O2 and H2O2 in anoxic medium (radiotherapy with heavy ions). A brief review of the measurements of superoxide free radicals in aqueous solution by indirect or direct methods is presented. Moreover, some experimental results obtained by pulse radiolysis with Ar18+ ions (TEL = 290 keV·µm-1), are described. The interpretation of the kinetics takes into account the superoxide absorbance and that of hydrogen peroxide, which is present at the millisecond time scale.Key words: pulse radiolysis, heavy ions, superoxide radicals, high LET, kinetics.[Traduit par la Rédaction]

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