Phospholipase Action in the Generation of Modified LDL

1994 ◽  
pp. 245-251
Author(s):  
Sampath Parthasarathy ◽  
Joellen Barnett
Keyword(s):  
Modified Ldl

Lysophosphatidylcholine Potentiates the Mitogenic Activity of Modified LDL for Human Monocyte–Derived Macrophages

1996 ◽  
Vol 16 (4) ◽  
pp. 600-605 ◽  
Author(s):  
Masakazu Sakai ◽  
Akira Miyazaki ◽  
Hideki Hakamata ◽  
Yoshihiro Sato ◽  
Takeshi Matsumura ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Mitogenic Activity ◽  
Modified Ldl

174 PROTECTION OF HDL AGAINST SPHINGOMYELINASE-MODIFIED LDL AGGREGATION AT ACIDIC PH

2011 ◽  
Vol 12 (1) ◽  
pp. 39
Author(s):  
S.D. Nguyen ◽  
K. Öörni ◽  
P.T. Kovanen
Keyword(s):  
Acidic Ph ◽  
Modified Ldl

scholarly journals Development of Capture Assays for Different Modifications of Human Low-Density Lipoprotein

2005 ◽  
Vol 12 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Gabriel Virella ◽  
M. Brooks Derrick ◽  
Virginia Pate ◽  
Charlyne Chassereau ◽  
Suzanne R. Thorpe ◽  
...  
Keyword(s):  
Oxidized Ldl ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Gas Chromatography Mass Spectrometry ◽  
Low Density ◽  
Modified Ldl ◽  
Capture Assay ◽  
Carboxymethyl Lysine

ABSTRACT Antibodies to malondialdehyde (MDA)-modified low-density lipoprotein (LDL), copper-oxidized LDL (oxLDL), N ε(carboxymethyl) lysine (CML)-modified LDL, and advanced glycosylation end product (AGE)-modified LDL were obtained by immunization of rabbits with in vitro-modified human LDL preparations. After absorption of apolipoprotein B (ApoB) antibodies, we obtained antibodies specific for each modified lipoprotein with unique patterns of reactivity. MDA-LDL antibodies reacted strongly with MDA-LDL and also with oxLDL. CML-LDL antibodies reacted strongly with CML-LDL and also AGE-LDL. oxLDL antibodies reacted with oxLDL but not with MDA-LDL, and AGE-LDL antibodies reacted with AGE-LDL but not with CML-LDL. Capture assays were set with each antiserum, and we tested their ability to capture ApoB-containing lipoproteins isolated from precipitated immune complexes (IC) and from the supernatants remaining after IC precipitation (free lipoproteins). All antibodies captured lipoproteins contained in IC more effectively than free lipoproteins. Analysis of lipoproteins in IC by gas chromatography-mass spectrometry showed that they contained MDA-LDL and CML-LDL in significantly higher concentrations than free lipoproteins. A significant correlation (r = 0.706, P < 0.019) was obtained between the MDA concentrations determined by chemical analysis and by the capture assay of lipoproteins present in IC. In conclusion, we have developed capture assays for different LDL modifications in human ApoB/E lipoprotein-rich fractions isolated from precipitated IC. This approach obviates the interference of IC in previously reported modified LDL assays and allows determination of the degree of modification of LDL with greater accuracy.

scholarly journals Angiotensin II Impairs Endothelial Nitric-oxide Synthase Bioavailability under Free Cholesterol-enriched Conditions via Intracellular Free Cholesterol-rich Membrane Microdomains

2013 ◽  
Vol 288 (20) ◽  
pp. 14497-14509 ◽  
Author(s):  
Eisuke Amiya ◽  
Masafumi Watanabe ◽  
Norihiko Takeda ◽  
Tetsuya Saito ◽  
Taro Shiga ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Endothelial Dysfunction ◽  
Free Cholesterol ◽  
Small Gtpase ◽  
Membrane Microdomains ◽  
No Production ◽  
Aortic Endothelial Cells ◽  
Modified Ldl ◽  
Rich Domain ◽  
Endothelial Nitric Oxide

Vascular endothelial function is impaired in hypercholesterolemia partly because of injury by modified LDL. In addition to modified LDL, free cholesterol (FC) is thought to play an important role in the development of endothelial dysfunction, although the precise mechanisms remain to be elucidated. The aim of this study was to clarify the mechanisms of endothelial dysfunction induced by an FC-rich environment. Loading cultured human aortic endothelial cells with FC induced the formation of vesicular structures composed of FC-rich membranes. Raft proteins such as phospho-caveolin-1 (Tyr-14) and small GTPase Rac were accumulated toward FC-rich membranes around vesicular structures. In the presence of these vesicles, angiotensin II-induced production of reactive oxygen species (ROS) was considerably enhanced. This ROS shifted endothelial NOS (eNOS) toward vesicle membranes and vesicles with a FC-rich domain trafficked toward perinuclear late endosomes/lysosomes, which resulted in the deterioration of eNOS Ser-1177 phosphorylation and NO production. Angiotensin II-induced ROS decreased the bioavailability of eNOS under the FC-enriched condition.

scholarly journals Exposure of Endothelial Cells to Physiological Levels of Myeloperoxidase-Modified LDL Delays Pericellular Fibrinolysis

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38810 ◽  
Author(s):  
Karim Zouaoui Boudjeltia ◽  
Jalil Daher ◽  
Pierre Van Antwerpen ◽  
Nicole Moguilevsky ◽  
Paul Delree ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Modified Ldl

Adhesion and migration of mononuclear cells in response to minimally modified LDL

2000 ◽  
Vol 151 (1) ◽  
pp. 188
Author(s):  
K.I. de Silva ◽  
N.G. Ardile ◽  
H.S. Warren
Keyword(s):  
Mononuclear Cells ◽  
Modified Ldl ◽  
And Migration

scholarly journals Mechanisms controlling competence gene expression in murine fibroblasts stimulated with minimally modified LDL.

1992 ◽  
Vol 12 (7) ◽  
pp. 800-806 ◽  
Author(s):  
R W Bork ◽  
K L Svenson ◽  
M Mehrabian ◽  
A J Lusis ◽  
A M Fogelman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Competence Gene ◽  
Modified Ldl ◽  
Murine Fibroblasts

Local Increase in Pai-2 on Stimulation of Monocytes with Modified Ldl

1995 ◽  
pp. 211-216
Author(s):  
Helen M. Ritchie ◽  
Alec Jamieson ◽  
Nuala A. Booth
Keyword(s):  
Local Increase ◽  
Modified Ldl ◽  
Stimulation Of

Meaning of a Modified LDL in Humans

1987 ◽  
pp. 209-212 ◽  
Author(s):  
Pietro Avogaro ◽  
Gabriele Bittolo Bon ◽  
Giuseppe Cazzolato
Keyword(s):  
Modified Ldl
Sign in / Sign up

Export Citation Format

Share Document