Chromatographic Techniques for the Characterization of Proteins

1995 ◽  
pp. 243-299 ◽  
Author(s):  
Joost J. M. Holthuis ◽  
Reinoud J. Driebergen
Keyword(s):  
Chromatographic Techniques

Extraction, isolation and characterization of Bauhinia variegata flower

2020 ◽  
pp. 9-12
Author(s):  
Akanksha Gupta ◽  
Abhishek K Tripathi ◽  
Pushpraj S Gupta
Keyword(s):  
Biological Activities ◽  
Molecular Formula ◽  
Native Plant ◽  
Compound A ◽  
Active Constituents ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Soxhlet Apparatus ◽  
Bauhinia Variegata

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc.  Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

Characterization of Multiblock Copolymers by Chromatographic Techniques

2011 ◽  
Vol 34 (2) ◽  
pp. 110-117 ◽  
Author(s):  
Patrick Yoba N'Goma ◽  
Wolfgang Radke ◽  
Frank Malz ◽  
Hans Jörg Ziegler ◽  
Michael Zierke ◽  
...  
Keyword(s):  
Multiblock Copolymers ◽  
Chromatographic Techniques

In-depth characterization of polyolefin plastomers/elastomers (ethylene/1-octene copolymers) through hyphenated chromatographic techniques

2020 ◽  
Vol 1621 ◽  
pp. 461081 ◽  
Author(s):  
J.-H. Arndt ◽  
R. Brüll ◽  
T. Macko ◽  
P. Garg ◽  
J.C.J.F. Tacx
Keyword(s):  
Chromatographic Techniques

scholarly journals Characterization of a Natural Dye by Spectroscopic and Chromatographic Techniques

2012 ◽  
Vol 1374 ◽  
pp. 49-59 ◽  
Author(s):  
Y. Espinosa-Morales ◽  
J. Reyes ◽  
B. Hermosín ◽  
J. A. Azamar-Barrios
Keyword(s):  
Molecular Structure ◽  
Phenolic Compounds ◽  
Central America ◽  
Structural Characterization ◽  
Chemical Properties ◽  
The Other ◽  
Chromatographic Techniques ◽  
Ft Ir ◽  
Color Source

ABSTRACTNatural dyes have been extracted from both plants and animal to give color to textiles and handicrafts. This is the case of purple dye extracted from Justicia spicigera Schldt, an acanthaceae used as a color source since pre-Hispanic period in the Mayan area of Mexico and Central America. Spectroscopic (UV-Vis and FT-IR) and chromatographic (PY-GC/MS) techniques were employed in order to characterize some of their chemical properties. UV-VIS absorption spectra indicates a λmaxpeak at 581 nm, value associated to anthocyanins group under bathochromic effect. On the other hand, a structural characterization realized by FT-IR and Py-GC/MS indicated the presence of polar hydroxibenzoic acids and phenolic compounds which are characteristics of the molecular structure of anthocyanins.

Characterization of naphthenic acids using mass spectroscopy and chromatographic techniques: study of technical mixtures

2014 ◽  
Vol 6 (3) ◽  
pp. 807-816 ◽  
Author(s):  
Flaviana C. Damasceno ◽  
Liliane D. A. Gruber ◽  
Ana M. Geller ◽  
Maria Cecília Vaz de Campos ◽  
Alexandre O. Gomes ◽  
...  
Keyword(s):  
Mass Spectroscopy ◽  
Naphthenic Acids ◽  
Chromatographic Techniques

scholarly journals Characterization of Natural Products from the Peel of Lagenaria siceraria Fruit Using Chromatographic Techniques

2017 ◽  
pp. 1-4 ◽  
Author(s):  
Inam Liaqat ◽  
Dildar Ahmed ◽  
Aimon Saleem ◽  
Rashid Masih ◽  
Raza Chaudhery
Keyword(s):  
Natural Products ◽  
Lagenaria Siceraria ◽  
Chromatographic Techniques

scholarly journals Cloning and Characterization of a New Site-Specific Methyl-Directed ElmI Endonuclease Recognizing and Cleaving C5-methylated DNA Sequence 5’-G(5mC)^NG(5mC)-3’

Acta Naturae ◽  
2016 ◽  
Vol 8 (1) ◽  
pp. 117-125 ◽  
Author(s):  
V. A. Chernukhin ◽  
D. A. Gonchar ◽  
M. A. Abdurashitov ◽  
O. A. Belichenko ◽  
V. S. Dedkov ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Amino Acid Sequences ◽  
Open Reading Frames ◽  
Site Specific ◽  
Pcr Screening ◽  
Methylated Dna ◽  
Chromatographic Techniques ◽  
Dna Fragment ◽  
Reading Frames

Putative open reading frames of MD-endonucleases have been identified in Enterobacteria genomes as a result of the search for amino acid sequences homologous to MD-endonuclease BisI. A highly conserved DNA primary structure of these open reading frames in different genera of Enterobacteria (Escherichia, Klebsiella and Cronobacter) has allowed researchers to create primers for PCR screening, which was carried out on Enterobacteria DNA collected from natural sources. The DNA fragment, about 440 bp in length, was amplified by use of the genomic DNA of a wild E.coli LM N17 strain as a template and was inserted into the pMTL22 vector. Endonuclease activity was detected in an E.coli ER 2267 strain transformed with the obtained construction. A new enzyme named ElmI was purified by chromatographic techniques from the recombinant strain biomass. It was discovered that similarly to BisI this enzyme specifically cleaves the methylated DNA sequence 5-GCNGC- 3 before the central nucleotide N if this sequence contains two 5-methylcytosines. However, unlike BisI, ElmI more efficiently cleaves this sequence if more than two cytosine residues are methylated.

scholarly journals Structural Characterization of the Symbiotically Important Low-Molecular-Weight Succinoglycan ofSinorhizobium meliloti

1999 ◽  
Vol 181 (21) ◽  
pp. 6788-6796 ◽  
Author(s):  
Lai-Xi Wang ◽  
Ying Wang ◽  
Brett Pellock ◽  
Graham C. Walker
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Sinorhizobium Meliloti ◽  
Structural Basis ◽  
Low Molecular Weight ◽  
Considerable Heterogeneity ◽  
Chromatographic Techniques ◽  
Detailed Structural Analysis ◽  
Derivatives Of

ABSTRACT The production of succinoglycan by Sinorhizobium meliloti Rm1021 is required for successful nodule invasion by the bacterium of its host plant, alfalfa. Rm1021 produces succinoglycan, an acidic exopolysaccharide composed of an octasaccharide repeating unit modified with acetyl, succinyl, and pyruvyl moieties, in both low- and high-molecular-weight forms. Low-molecular-weight (LMW) succinoglycan, previously thought to consist of monomers, trimers, and tetramers of the repeating unit, has been reported as being capable of promoting the formation of nitrogen-fixing nodules by succinoglycan-deficient derivatives of strain Rm1021. We have determined that the three size classes of LMW succinoglycan species are in fact monomers, dimers, and trimers of the repeating unit and that the trimer is the species active in promoting nodule invasion. A detailed structural analysis of the components of LMW succinoglycan by using various chromatographic techniques, along with nuclear magnetic resonance analyses, has revealed that there is considerable heterogeneity within the LMW succinoglycan oligomers in terms of noncarbohydrate substitutions, and we have determined the structural basis of this heterogeneity.

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