Extraction, isolation and characterization of Bauhinia variegata flower

2020 ◽  
pp. 9-12
Author(s):  
Akanksha Gupta ◽  
Abhishek K Tripathi ◽  
Pushpraj S Gupta
Keyword(s):  
Biological Activities ◽  
Molecular Formula ◽  
Native Plant ◽  
Compound A ◽  
Active Constituents ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Soxhlet Apparatus ◽  
Bauhinia Variegata

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc.  Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

scholarly journals Isolation and characterization of aristolactam alkaloids from the stem bark of Goniothalamus velutinus (Airy Shaw) and their biological activities

2018 ◽  
Vol 30 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Erum Iqbal ◽  
Linda B.L. Lim ◽  
Kamariah Abu Salim ◽  
Shaheen Faizi ◽  
Ayaz Ahmed ◽  
...  
Keyword(s):  
Biological Activities ◽  
Stem Bark ◽  
Isolation And Characterization

A review on phyto-pharmacology of Oxalis corniculata

2021 ◽  
Vol 24 ◽  
Author(s):  
Iqra Sarfraz ◽  
Azhar Rasul ◽  
Ghulam Hussain ◽  
Muhammad Ajmal Shah ◽  
Bushra Nageen ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Current Effort ◽  
Isolation And Characterization ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Warm Temperate

: Oxalis corniculata (Oxalidaceae) is a small decumbent and delicate appearing medicinal herb flourishing in warm temperate and tropical domains such as Pakistan and India. Main bioactive chemical constituents of Oxalis plant include several alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins along with steroids. Due to its polyphenolic, glycosides and flavonoid profile, it is proved to be protective in numerous ailments and exhibit various biological activities such as anti-fungal, anti-cancer, anti-oxidant, anti-bacterial, anti-diabetic, and cardioprotective. Moreover, bioactive phytochemicals from this plant possess significant wound healing potential. Our current effort intends to emphasize on the immense significance of this plant species, which have not been the subject matter of clinical trials and effective pharmacological studies, even though its favored usage has been stated. This review proposes that Oxalis corniculata possess potential for the cure of various diseases, however, further researches on isolation and characterization of bioactive compounds along with pre-clinical trials are compulsory to figure out its pharmacological applications.

scholarly journals The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 847 ◽  
Author(s):  
Sónia A. O. Santos ◽  
Rafael Félix ◽  
Adriana C. S. Pais ◽  
Sílvia M. Rocha ◽  
Armando J. D. Silvestre
Keyword(s):  
Phenolic Compounds ◽  
Biological Activities ◽  
Mass Spectrometry Data ◽  
Brown Macroalgae ◽  
Isolation And Characterization ◽  
Analytical Strategies ◽  
Critical Revision ◽  
Identification Techniques ◽  
First Time

The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.

Synthesis of low-symmetry subphthalocyanines with diverse functionalization patterns

2009 ◽  
Vol 13 (02) ◽  
pp. 203-214 ◽  
Author(s):  
David González-Rodríguez ◽  
Christian G. Claessens ◽  
Tomás Torres
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectra ◽  
Careful Analysis ◽  
Complete Separation ◽  
H Nmr ◽  
Chromatographic Techniques ◽  
Structural Assignment ◽  
Isolation And Characterization ◽  
Low Symmetry

We report here on the synthesis, isolation and characterization of unsymmetrically substituted subphthalocyanines obtained from the mixed condensation of 4,5-substituted phthalonitriles and/or 4-substituted phthalonitriles in the presence of BCl 3. Compared to phthalocyanines, it is clear that the cone-shaped structure of subphthalocyanines facilitates the isolation of the different regioisomers formed by regular laboratory chromatographic techniques. However, we demonstrate that some factors, such as the solubility of the regioisomers formed or the intermolecular interactions between side chains, can sometimes impede their complete separation. We also show that the structural assignment of each regioisomeric compound can be performed by careful analysis of its 1 H NMR spectra and, in some cases, with the help of NOE NMR experiments and molecular modeling.

Isolation and characterization of β-lipolytic hormone from porcine pituitary gland

1970 ◽  
Vol 48 (9) ◽  
pp. 1017-1021 ◽  
Author(s):  
C. Gilardeau ◽  
M. Chrétien
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Pituitary Gland ◽  
Amino Acid Composition ◽  
Biological Activities ◽  
Terminal Amino Acid ◽  
Isolation And Characterization ◽  
Pituitary Glands ◽  
Terminal Amino

A lipolytic substance was isolated from porcine pituitary glands. It's amino acid composition, molecular weight, N-terminal amino acid, isoelectric point, and biological activities are reported. These results are compared to the corresponding values of sheep β-lipolytic hormone.

scholarly journals Chemical constituents of leaves Dialium cochinchinense Pierre

2021 ◽  
Vol 4 (3) ◽  
pp. 1131-1136
Author(s):  
Vu Thi Huyen ◽  
Doan Thi Thuy Ai ◽  
Nguyen Thi Hien
Keyword(s):  
Secondary Metabolites ◽  
Nmr Spectra ◽  
Spectroscopic Analysis ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Tropical Regions ◽  
First Report ◽  
Isolation And Characterization

The genus Dialium belongs to the Caesalpinioideae family, consisting of approximately 30 species distributed in the tropical regions. Secondary metabolites from the  Dialium genus have been reported to exhibit various biological activities including antioxidant, cytotoxicity and antimicrobial activities. This work describes the isolation and characterization of five compounds from the leaves of Dialium cochinchinense Pierre. Their structures were established by spectroscopic analysis, including MS and NMR spectra. Accordingly, the isolated compounds were identified to be lupeone (1), b-sitostenone (2), β-sitosterol (3), daucosterol (4), and dihydrokaempferide (5). To the best of our knowledge, this is the first report of the isolation of compounds 1 and 5 from the genus Dialium.

Isolation and Characterization of Cytotoxic Diterpenoid Isomers from Propolis

1997 ◽  
Vol 52 (9-10) ◽  
pp. 702-704 ◽  
Author(s):  
Tetsuya Matsuno ◽  
Yasuyuki Matsumoto ◽  
Masahiro Saito ◽  
Junji Morikawa
Keyword(s):  
Methanol Extract ◽  
Carcinoma Cell ◽  
Molecular Formula ◽  
Cell Cytotoxicity ◽  
Isolation And Characterization ◽  
Clerodane Diterpenoids ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Brazilian Propolis

The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-n-oic acid)

Isolation and characterization of jadomycin L from Streptomyces venezuelae ISP5230 for solid tumor efficacy studies

2009 ◽  
Vol 81 (6) ◽  
pp. 1041-1049 ◽  
Author(s):  
David L. Jakeman ◽  
Stephanie N. Dupuis ◽  
Cathy L. Graham
Keyword(s):  
Natural Products ◽  
Solid Tumor ◽  
Culture Media ◽  
Biological Activities ◽  
Streptomyces Venezuelae ◽  
Gram Positive Bacteria ◽  
Isolation And Characterization ◽  
One Step ◽  
Efficacy Studies

Precursor-directed biosynthesis offers opportunities to modify natural products and obtain structurally complex metabolites without the need for chemical synthesis. However, such opportunities are limited owing to the inherent substrate specificity of biosynthetic enzymes. The jadomycins are a family of natural products produced by the soil microbe Streptomyces venezuelae ISP5230. Their biosynthesis contains one step that is potentially non-enzymatic, namely, the condensation of a biosynthetic aldehyde and an amino acid that leads to a uniquely substituted oxazolone ring. Variation of amino acids in the culture media enables the production of a wide array of substituted oxazolones. These analogs have been shown to have a variety of biological activities against cancer cell lines and also against Gram-positive bacteria. Herein, we report the first isolation and characterization of jadomycin L and jadomycin L aglycone from 8 L of bacterial culture for solid tumor efficacy studies.

scholarly journals Chemistry, Biological Activities and In Silico Bioprospection of Sterols and Triterpenes from Mexican Columnar Cactaceae

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1649 ◽  
Author(s):  
Juan Rodrigo Salazar ◽  
Marco A. Loza-Mejía ◽  
Diego Soto-Cabrera
Keyword(s):  
In Silico ◽  
Molecular Mechanisms ◽  
Metabolic Diseases ◽  
Biological Activities ◽  
Biological Properties ◽  
Degenerative Diseases ◽  
Growth Forms ◽  
Isolation And Characterization ◽  
Live Fences

The Cactaceae family is an important source of triterpenes and sterols. The wide uses of those plants include food, gathering, medicinal, and live fences. Several studies have led to the isolation and characterization of many bioactive compounds. This review is focused on the chemistry and biological properties of sterols and triterpenes isolated mainly from some species with columnar and arborescent growth forms of Mexican Cactaceae. Regarding the biological properties of those compounds, apart from a few cases, their molecular mechanisms displayed are not still fully understand. To contribute to the above, computational chemistry tools have given a boost to traditional methods used in natural products research, allowing a more comprehensive exploration of chemistry and biological activities of isolated compounds and extracts. From this information an in silico bioprospection was carried out. The results suggest that sterols and triterpenoids present in Cactaceae have interesting substitution patterns that allow them to interact with some bio targets related to inflammation, metabolic diseases, and neurodegenerative processes. Thus, they should be considered as attractive leads for the development of drugs for the management of chronic degenerative diseases.

Research Progress on Ways of Secondary Metabolism in Plants

2012 ◽  
Vol 178-181 ◽  
pp. 1004-1007
Author(s):  
Xiu Bo Liu ◽  
Yan Shu Jia ◽  
Na Li ◽  
He Gong ◽  
Yan He Zhang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Biological Activities ◽  
Natural Habitat ◽  
Chemical Compounds ◽  
Research Progress ◽  
Primary Metabolites ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Anti Cancer

The function of secondary metabolites has been a subject of debate since the first isolation and characterization of these often times structurally complex chemical compounds. By definition, secondary metabolites are naturally produced compounds that are not essential for the survival of the producing organism, as opposed to primary metabolites. Bioassays have revealed a wide range of biological activities, such as cytotoxicity, ichthyotoxicity, antimicrobial and antifeedant activity, antifoulant and anti-cancer activities. However, some of these activities are manifested against organisms that do not represent logical targets, since they would never be encountered by the producing organism in their natural habitat.

Sign in / Sign up

Export Citation Format

Share Document