Distribution of Individual Cytoplasmic pH Values in a Cell Suspension

1996 ◽  
pp. 91-93
Author(s):  
Petr Cimprich ◽  
Jan Slavík
Keyword(s):  
Cell Suspension ◽  
Cytoplasmic Ph ◽  
Ph Values ◽  
A Cell

Experimental study of diffusion-based extraction from a cell suspension

2008 ◽  
Vol 5 (4) ◽  
pp. 529-540 ◽  
Author(s):  
Clara Mata ◽  
Ellen K. Longmire ◽  
David H. McKenna ◽  
Katie K. Glass ◽  
Allison Hubel
Keyword(s):  
Experimental Study ◽  
Cell Suspension ◽  
A Cell

scholarly journals The use of a slide spinner in the analysis of cell dispersion.

1976 ◽  
Vol 24 (1) ◽  
pp. 11-15 ◽  
Author(s):  
R C Wolley ◽  
H M Dembitzer ◽  
F Herz ◽  
K Schreiber ◽  
L G Koss
Keyword(s):  
Cell Suspension ◽  
Single Cells ◽  
Cell Loss ◽  
Optimum Conditions ◽  
Isolated Cells ◽  
Cell Dispersion ◽  
Cervical Cancer Cells ◽  
A Cell ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

A simple and reliable method of determining the degree of dispersion of a cell suspension has been developed using the Perkin-Elmer Uni-Smear Spinner. Optimum conditions regarding rate and duration of spin, etc., were first ascertained using dispersed cell cultures including human cervical cancer cells as well as gynecologic samples. After spinning, single cells in suspension appeared as isolated cells on the slides. Cell aggregates, on the other hand, remained together. Therefore, the distribution of cells in various sized aggregates could be easily quantitated and the slides retained for future review. This method was used to evaluate the dispersing effects of trypsin, ethylenediaminetetraacetate and and syringing human on human gynecology samples obtained by routine cervical scrapes. None of the dispersion methods has, so far, produced an adequate monodispersed cell suspension without unacceptable cell loss.

scholarly journals ESTABLISHMENT OF A CELL SUSPENSION CULTURE FROM Calophyllum brasiliense AND EVALUATION OF ITS ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY

2019 ◽  
Vol 19 (1) ◽  
pp. 59-70
Author(s):  
D. Cisneros-Tórres ◽  
◽  
F. Cruz-Sosa ◽  
M.P. Nicasio-Tórres ◽  
M. González-Cortazar ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Inflammatory Activity ◽  
Calophyllum Brasiliense ◽  
A Cell ◽  
Anti Inflammatory

Accumulation and Biotransformation of Chromenes and Benzofurans in a Cell Suspension Culture ofAgeratina adenophora

Planta Medica ◽  
1987 ◽  
Vol 53 (05) ◽  
pp. 488-492 ◽  
Author(s):  
Peter Proksch ◽  
Ludger Witte ◽  
Victor Wray ◽  
Ines Rahaus
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
A Cell

Analyse de la morphogenèse du pied des Oiseaux à l'aide de mélanges cellulaires interspécifiques

Development ◽  
1973 ◽  
Vol 29 (1) ◽  
pp. 175-196
Author(s):  
Par Marie-Paule Pautou
Keyword(s):  
Cell Suspension ◽  
The Other ◽  
Composite Type ◽  
A Cell ◽  
Species Specific ◽  
Host Embryo

Morphogenesis of the feet of birds, studied in limbs developed from reaggregated heterospecific mesoderm Experiments were undertaken to determine whether species-specific characters of chick and duck mesodermal leg-bud cells are retained after dissociation and reaggregation in homoand heterospecific mixtures. Prospective zeugopod and autopod mesoderm from chick and/or duck leg buds were isolated, dissociated into a cell suspension and pelleted by centrifugation. The reaggregated mesoderm was packed into a leg-bud ectodermal jacket; the recombined leg bud was then grafted on the wing stump of a host embryo. Recombinants whose mesoderm was a homospecific reaggregate developed into typical chick or duck leg parts according to the specific origin of the mesodermal component; the feet of nearly all these legs lacked antero-posterior polarity. Recombinants containing heterospecific reaggregates were also capable of forming reasonably organized leg structures. The foot was not, as a rule, of the specific type expected of the majority component. In a mixture of 75% chick mesoderm cells and 25% duck mesoderm cells, the feet which developed were either of chick type or of composite chick/duck type, where typical chick areas were next to typical or aberrant (steganoid) duck areas. When the ratio was reversed (25% chick, 75% duck), the majority of the feet were again of chick type or of composite chick/duck type, the typical duck phenotype being exceptional. Even in a mixture of 10% chick cells and 90% duck cells, duck-type feet were not obtained. They were all of composite type: half of their interdigital zones were of chick type, the other half were occupied, in most cases, by underdeveloped, indented webbing or by one or several discrete flaps, and, in a few cases, by normal webbing. The vast majority of the feet developed from heterospecific mesoderm were characterized by the profusion of the toes, which were not polarized along the a–p axis.

scholarly journals Lupeol acetate production and antioxidant activity of a cell suspension culture from Cnidoscolus chayamansa leaves

2019 ◽  
Vol 125 ◽  
pp. 30-38 ◽  
Author(s):  
M.Z. Pérez-González ◽  
A. Nieto-Trujillo ◽  
G.A. Gutiérrez-Rebolledo ◽  
I. García-Martínez ◽  
M.E. Estrada-Zúñiga ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Acetate Production ◽  
A Cell ◽  
Lupeol Acetate ◽  
Cnidoscolus Chayamansa

Growth Behavior of a Liverwort, Jungermannia subulata Evans, in a Cell Suspension Culture. The Role of Organic Acids Required for Cell Growth

1981 ◽  
Vol 22 (8) ◽  
pp. 1533-1540 ◽  
Author(s):  
Yoshimoto Ohta ◽  
Momoyo Ishikawa ◽  
Setsuko Abe ◽  
Kenji Katoh ◽  
Yoshio Hirose
Keyword(s):  
Cell Growth ◽  
Organic Acids ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Growth Behavior ◽  
A Cell

scholarly journals Excitation, adaption, and deadaptation of the cAMP-mediated cGMP Response in dictyostelium discoideum

1983 ◽  
Vol 96 (2) ◽  
pp. 347-353 ◽  
Author(s):  
PJM Van Haaster ◽  
PR Van Der Heijden
Keyword(s):  
Cell Suspension ◽  
Dictyostelium Discoideum ◽  
Half Life ◽  
Cell Aggregation ◽  
First Order ◽  
Intracellular Cgmp ◽  
First Order Kinetics ◽  
A Cell ◽  
Stimulation Period ◽  
Extracellular Camp

Extracellular cAMP induces chemotaxis and cell aggregation in dictyostelium discoideum cells. cAMP added to a cell suspension is rapidly hydrolyzed (half-life of 10 s) and induces a rapid increase of intracellular cGMP levels, which reach a peak at 10 s and recover prestimulated levels at about 30 s. This recovery is not due to removal of the stimulus because the nonhydrolyzable analogue adenosine 3',5'-monophosphorothioate-Sp- stereoisomer (cAMPS) induced a comparable cGMP response, which peaked at 10 s, even at subsaturating cAMPS concentrations. When cells were stimulated twice with the same cAMP concentration at a 30-s interval, only the first stimulus produced a cGMP response. Cells did respond to the second stimulus when the concentration of the second stimulus was higher than that of the first stimulus. By increasing the interval between two identical stimuli, the response to the second stimulus gradually increased. Recovery from the first stimulus showed first-order kinetics with a half-life of 1-2 min. The stimulation period was shortened by adding phosphodieterase to the cell suspension. The cGMP response was unaltered if the half-life of cAMP was reduced to 2 S. The peak of the transient cGMP accumulation still appeared at 10 s even when the half- life of cAMP was 0.4 s; however, the height of the cGMP peak was reduced. The cGMP response at 10 s after stimulation was diminished by 50 percent when the half-life of 10(-7) M cAMP was 0.5 s or when the half-life of 10(-8) M cAMP was 3.0 s. These results show that the cAMP signal is transduced to two opposing processes: excitation and adaptation. Within 10 s after addition of cAMP to a cell suspension the level of adaptation reaches the level of excitation, which causes the extinction of the transduction of the signal. Deadaptation starts as soon as the signal is removed, and it has first-order kinetics with a half-life of 1-2 min.

Purification of human monocyte-macrophages by a cell suspension culture technique

1980 ◽  
Vol 53 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Henry C. Stevenson ◽  
Paul Katz ◽  
Anthony S. Fauci
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Human Monocyte ◽  
Culture Technique ◽  
A Cell
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