Low-Temperature Light Microscopy

1992 ◽  
pp. 265-293 ◽  
Author(s):  
Patrick Echlin
Keyword(s):  
Low Temperature ◽  
Light Microscopy

Low temperature light microscopy and its application to study freezing in aqueous solutions and biological cell suspensions

1986 ◽  
Vol 141 (3) ◽  
pp. 263-276 ◽  
Author(s):  
Ch. Körber ◽  
S. Englich ◽  
P. Schwindke ◽  
M. W. Scheiwe ◽  
G. Rau ◽  
...  
Keyword(s):  
Low Temperature ◽  
Light Microscopy ◽  
Aqueous Solutions ◽  
Cell Suspensions ◽  
Biological Cell

A Method to Study Intracellular Ice Nucleation

1998 ◽  
Vol 120 (1) ◽  
pp. 27-31 ◽  
Author(s):  
K. Tatsutani ◽  
B. Rubinsky
Keyword(s):  
Low Temperature ◽  
Light Microscopy ◽  
Cancer Cells ◽  
Directional Solidification ◽  
Thermal History ◽  
Prostatic Cancer ◽  
Ice Nucleation ◽  
Cell Destruction ◽  
New Apparatus ◽  
Intracellular Ice

The thermodynamics of intracellular ice nucleation are important in low-temperature biology for understanding and controlling the process of cell destruction by freezing. We have developed a new apparatus and technique for studying the physics of intracellular ice nucleation. Employing the principle of directional solidification in conjunction with light microscopy, we can generate information on the temperature at which ice nucleates intracellularly as a function of the thermal history the cells experience. The methodology is introduced, and results with primary prostatic cancer cells are described.

The design and use of a low-temperature stage for light microscopy

Cryobiology ◽  
1980 ◽  
Vol 17 (6) ◽  
pp. 615 ◽  
Author(s):  
D.S. Reid ◽  
W.F. Rall ◽  
M. Rosenthal
Keyword(s):  
Low Temperature ◽  
Light Microscopy

scholarly journals Low-temperature fabrication and characterization of a symmetric hybrid organic–inorganic slab waveguide for evanescent light microscopy

Nano Futures ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 025007 ◽  
Author(s):  
Björn Agnarsson ◽  
Mokhtar Mapar ◽  
Mattias Sjöberg ◽  
Mohammadreza Alizadehheidari ◽  
Fredrik Höök
Keyword(s):  
Low Temperature ◽  
Light Microscopy ◽  
Slab Waveguide ◽  
Evanescent Light ◽  
Fabrication And Characterization

scholarly journals Development of a simple embedding procedure allowing immunocytochemical localization at the ultrastructural level.

1988 ◽  
Vol 36 (12) ◽  
pp. 1579-1582 ◽  
Author(s):  
G Escolar ◽  
J J Sauk ◽  
M L Bravo ◽  
M Krumwiede ◽  
J G White
Keyword(s):  
Low Temperature ◽  
Light Microscopy ◽  
Uv Light ◽  
Ultrastructural Level ◽  
Microscopic Level ◽  
Water Soluble ◽  
Immunocytochemical Localization ◽  
Protein Antigens ◽  
Light Microscopic Level ◽  
Thin Sections

Immunobed solution A is a water-soluble acrylic compound recently developed for immunocytochemical localization at the light microscopic level. In this study, we combined it with methyl methacrylate (MMA) to achieve sufficient hardness to obtain ultra-thin sections. Samples of platelets were dehydrated and embedded in the water-soluble acrylic mixture (WSAM). The embedding process was carried out at 4 degrees C and final polymerization was induced with either chemical (benzoyl peroxide) or physical (UV light) catalysts. Tubulin was localized at the ultrastructural level in sections embedded according to these two methods. Results were compared with those obtained in platelets processed in Lowicryl. Dehydration and embedding with the WSAM yielded a preservation of antigenicity similar to that obtained in Lowicryl. The new procedure benefits from the low temperature achieved during polymerization, providing good ultrastructural morphology and immunolocalization of protein antigens with the simplicity of a routine embedding procedure for light microscopy.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

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