Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF

2014 ◽  
pp. 53-69 ◽  
Author(s):  
Edgar González-Buendía ◽  
Rosario Pérez-Molina ◽  
Erandi Ayala-Ortega ◽  
Georgina Guerrero ◽  
Félix Recillas-Targa
Keyword(s):  
Experimental Strategies

scholarly journals A roadmap for metagenomic enzyme discovery

2021 ◽  
Author(s):  
Serina L. Robinson ◽  
Jörn Piel ◽  
Shinichi Sunagawa
Keyword(s):  
Biosynthetic Enzymes ◽  
Functional Metagenomics ◽  
Enzyme Discovery ◽  
Experimental Strategies

Shotgun metagenomic approaches to uncover new enzymes are underdeveloped relative to PCR- or activity-based functional metagenomics. Here we review computational and experimental strategies to discover biosynthetic enzymes from metagenomes.

Experimental strategies on enhancing toxic gases uptake of metal–organic frameworks

2021 ◽  
Vol 430 ◽  
pp. 213738
Author(s):  
Ehsan Binaeian ◽  
El-Sayed M. El-Sayed ◽  
Mojtaba Khanpour Matikolaei ◽  
Daqiang Yuan
Keyword(s):  
Metal Organic Frameworks ◽  
Toxic Gases ◽  
Metal Organic ◽  
Experimental Strategies

scholarly journals Enabling Proteomics: The Need for an Extendable ‘Workbench’ for User-Configurable Solutions

2004 ◽  
Vol 5 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Robert J. Beynon
Keyword(s):  
Data Analysis ◽  
Data Reduction ◽  
Quantitative Data ◽  
Data Gathering ◽  
Qualitative And Quantitative ◽  
Proteomics Tool ◽  
Experimental Strategies

Proteomics has the capability to generate overwhelming quantities of data in relatively short timescales, and it is not uncommon to see experimenters investing substantially more time in data analysis than in data gathering. Although several sophisticated tools for data reduction and analysis are available, they lack the flexibility to cope with increasingly innovative experimental strategies and new database resources that encode both qualitative and quantitative data. I will outline a specification of a flexible proteomics tool that could address many current bottlenecks and deficiencies.

Crystal size control of TiO2 using experimental strategies in sol–gel process

2010 ◽  
Vol 5 (6) ◽  
pp. 361 ◽  
Author(s):  
R.-J. Tan ◽  
Y.-H. Tseng ◽  
C.-H. Kuo
Keyword(s):  
Crystal Size ◽  
Sol Gel ◽  
Size Control ◽  
Sol Gel Process ◽  
Experimental Strategies

Mesenchymal stem cells immortalization's and state-of-art: a systematic review and meta-analysis

2021 ◽  
Vol 31 (Supplement_2) ◽  
Author(s):  
P E F Stricker ◽  
A C Irioda ◽  
B F Mogharbel ◽  
E Abdelwaid ◽  
L R Cavalli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Transfection ◽  
Meta Analysis ◽  
Total N ◽  
Htert Gene ◽  
Cell Immortalization ◽  
Immortalized Cell ◽  
Experimental Strategies ◽  
Established Technique

Abstract Background The immortalization of mesenchymal stem cells (MSCs) allows them to avoid senescence and be cultured through limitless cell passages. Thus, several experimental strategies, such as retrovirus-mediated gene transfer or viral oncogenesis, have been applied for the immortalization of MSCs. The aim was to identifier the most commonly used methodologies and their particularities for the immortalization of human and animal MSCs. Methods The search was conducted in June 2019 and developed in SCOPUS, PUBMED, and SCIENCE DIRECT. Statistical analysis was performed, obtaining the values of total n, mean and standard deviation, confidence interval (CI), and percentage (frequency) for all the predictors. Results The most used immortalization methodology was viral transfection, being the most common immortalized cell type was the bone marrow-derived MSC, and the most used gene for immortalizing both human and animal MSCs was hTERT (39.3%) and SV40T (54.5%). Among the articles analyzed in this review, only 39.3% and 36.4% of human and animal MSCs immortalization protocols, respectively, underwent the tumorigenicity test. Conclusions The virus-mediated gene transfection was observed as the most used and established technique. The insertion of the hTERT gene is still the most used gene for cell immortalization, suggesting that the maintenance of telomerase is efficient for maintaining cell proliferation and bypassing cell senescence. The review concluded that the tumorigenicity tests should become mandatory in order to safely use the immortalized MSCs for translation.

Novel Experimental Strategies to Prevent the Development of Type 1 Diabetes Mellitus

2000 ◽  
Vol 105 (2) ◽  
pp. 17-34 ◽  
Author(s):  
Stellan Sandler ◽  
Annika K. Andersson ◽  
Andreea Barbu ◽  
Claes Hellerström ◽  
Maria Holstad ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Experimental Strategies

An Applied Behavior Analysis Research Primer for Behavioral Change Personnel

1981 ◽  
Vol 6 (3) ◽  
pp. 164-174 ◽  
Author(s):  
Mark A. Koorland ◽  
David L. Westling
Keyword(s):  
Behavior Analysis ◽  
Applied Behavior Analysis ◽  
Behavioral Change ◽  
Multiple Baseline ◽  
Research Strategies ◽  
Technical Aspects ◽  
Research Outcomes ◽  
Research Designs ◽  
Experimental Logic ◽  
Experimental Strategies

Assistance for the consumer in understanding Applied Behavior Analysis (ABA) research strategies and technical aspects is provided. Various measures of behavior and reliability are discussed. Experimental strategies and variations of reversal and multiple baseline research designs are reviewed with particular attention given to experimental logic. It is felt if the consumer understands the principles guiding researchers, then accurate appraisal and useful applications of ABA research outcomes are likely.

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