scholarly journals mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution

2015 ◽  
pp. 133-147 ◽  
Author(s):  
Laurent Chatre ◽  
Miria Ricchetti
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Dna Dynamics ◽  
Imaging Tool

scholarly journals 3P325 Direct quantification of mitochondria and mitochondrial DNA dynamics in living cells(Bioimaging,The 48th Annual Meeting of the Biophysical Society of Japan)

2010 ◽  
Vol 50 (supplement2) ◽  
pp. S202
Author(s):  
Yasutomo Nomura ◽  
Tomomi Nabeya ◽  
Kyohei Nakayama ◽  
Kazuki Nishimoto ◽  
Yui Januma ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Annual Meeting ◽  
Living Cells ◽  
Dna Dynamics ◽  
Biophysical Society ◽  
Direct Quantification

scholarly journals Identification of unique and shared mitochondrial DNA mutations in neurodegeneration and cancer by single-cell mitochondrial DNA structural variation sequencing (MitoSV-seq)

EBioMedicine ◽  
2020 ◽  
Vol 57 ◽  
pp. 102868
Author(s):  
Elham Jaberi ◽  
Emilie Tresse ◽  
Kirsten Grønbæk ◽  
Joachim Weischenfeldt ◽  
Shohreh Issazadeh-Navikas
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Structural Variation ◽  
Mitochondrial Dna Mutations ◽  
Dna Mutations

scholarly journals AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

Scanning ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Mohamed Yassine Amarouch ◽  
Jaouad El Hilaly ◽  
Driss Mazouzi
Keyword(s):  
Single Cell ◽  
Protein Interactions ◽  
Single Cells ◽  
Cardiac Cells ◽  
Cellular Biology ◽  
Adhesion Forces ◽  
Cell Organelles ◽  
Force Microscopy ◽  
Imaging Tool ◽  
Atomic Force

Atomic force microscopy (AFM) is a widely used imaging technique in material sciences. After becoming a standard surface-imaging tool, AFM has been proven to be useful in addressing several biological issues such as the characterization of cell organelles, quantification of DNA-protein interactions, cell adhesion forces, and electromechanical properties of living cells. AFM technique has undergone many successful improvements since its invention, including fluidic force microscopy (FluidFM), which combines conventional AFM with microchanneled cantilevers for local liquid dispensing. This technology permitted to overcome challenges linked to single-cell analyses. Indeed, FluidFM allows isolation and injection of single cells, force-controlled patch clamping of beating cardiac cells, serial weighting of micro-objects, and single-cell extraction for molecular analyses. This work aims to review the recent studies of AFM implementation in molecular and cellular biology.

scholarly journals Heteroplasmy variability in individuals with biparentally inherited mitochondrial DNA

2020 ◽  
Author(s):  
Jesse Slone ◽  
Weiwei Zou ◽  
Shiyu Luo ◽  
Eric S Schmitt ◽  
Stella Maris Chen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Strong Evidence ◽  
Genetic Factors ◽  
Single Cell Level ◽  
Biparental Inheritance ◽  
Cell Level ◽  
Single Population ◽  
Parent Of Origin ◽  
Two Populations

ABSTRACTWith very few exceptions, mitochondrial DNA (mtDNA) in humans is transmitted exclusively from mothers to their offspring, suggesting the presence of a strong evolutionary pressure favoring the exclusion of paternal mtDNA. We have recently shown strong evidence of paternal mtDNA transmission. In these rare situations, males exhibiting biparental mtDNA appear to be limited to transmitting just one of the mtDNA species to their offspring, while females possessing biparental mtDNA populations consistently transmit both populations to their offspring at a very similar heteroplasmy level. The precise biological and genetic factors underlying this unusual transmission event remain unclear. Here, we have examined heteroplasmy levels in various tissues among individuals with biparental inheritance. Our results indicate that individuals with biparental mtDNA have remarkable inter-tissue variability in heteroplasmy level. At the single-cell level, paternal mtDNA heteroplasmy in sperm varies dramatically, and many sperm possess only one of the two mtDNA populations originally in question. These results show a fundamental, parent-of-origin difference in how mtDNA molecules transmit and propagate. This helps explain how a single population of mtDNAs are transmitted from a father possessing two populations of mtDNA molecules, suggesting that some mtDNA populations may be favored over others when transmitted from the father.

scholarly journals Understanding mitochondrial DNA maintenance disorders at the single muscle fibre level

2019 ◽  
Vol 47 (14) ◽  
pp. 7430-7443 ◽  
Author(s):  
Diana Lehmann ◽  
Helen A L Tuppen ◽  
Georgia E Campbell ◽  
Charlotte L Alston ◽  
Conor Lawless ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Respiratory Chain ◽  
Genetic Defect ◽  
Clear Correlation ◽  
Muscle Fibres ◽  
Single Muscle Fibre ◽  
Respiratory Chain Deficiency ◽  
Mtdna Deletions ◽  
Mtdna Deletion

Abstract Clonal expansion of mitochondrial DNA (mtDNA) deletions is an important pathological mechanism in adults with mtDNA maintenance disorders, leading to a mosaic mitochondrial respiratory chain deficiency in skeletal muscle. This study had two aims: (i) to determine if different Mendelian mtDNA maintenance disorders showed similar pattern of mtDNA deletions and respiratory chain deficiency and (ii) to investigate the correlation between the mitochondrial genetic defect and corresponding respiratory chain deficiency. We performed a quantitative analysis of respiratory chain deficiency, at a single cell level, in a cohort of patients with mutations in mtDNA maintenance genes. Using the same tissue section, we performed laser microdissection and single cell genetic analysis to investigate the relationship between mtDNA deletion characteristics and the respiratory chain deficiency. The pattern of respiratory chain deficiency is similar with different genetic defects. We demonstrate a clear correlation between the level of mtDNA deletion and extent of respiratory chain deficiency within a single cell. Long-range and single molecule PCR shows the presence of multiple mtDNA deletions in approximately one-third of all muscle fibres. We did not detect evidence of a replicative advantage for smaller mtDNA molecules in the majority of fibres, but further analysis is needed to provide conclusive evidence.

Live-Cell Probe for In Situ Single-Cell Monitoring of Mitochondrial DNA Methylation

ACS Sensors ◽  
2021 ◽  
Author(s):  
Han Zhao ◽  
Donghan Ma ◽  
Junkai Xie ◽  
Oscar Sanchez ◽  
Fang Huang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Single Cell ◽  
Live Cell ◽  
Cell Monitoring

scholarly journals Perioperative plasma mitochondrial DNA dynamics and correlation with inflammation during infantile cardiopulmonary bypass

2017 ◽  
Vol 69 (6) ◽  
pp. 797-800 ◽  
Author(s):  
Fei Xu ◽  
Rui-qi Liu ◽  
Rong Cao ◽  
Lang-tao Guo ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cardiopulmonary Bypass ◽  
Dna Dynamics
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