Quantitative Analysis of Protein-Protein Interactions

2015 ◽  
pp. 23-37 ◽  
Author(s):  
Ziad M. Eletr ◽  
Keith D. Wilkinson
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Protein Protein Interactions

Micropatterning for quantitative analysis of protein-protein interactions in living cells

2008 ◽  
Vol 5 (12) ◽  
pp. 1053-1060 ◽  
Author(s):  
Michaela Schwarzenbacher ◽  
Martin Kaltenbrunner ◽  
Mario Brameshuber ◽  
Clemens Hesch ◽  
Wolfgang Paster ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions

scholarly journals Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors

2011 ◽  
Vol 67 (3) ◽  
pp. 542-553 ◽  
Author(s):  
Christian Gehl ◽  
David Kaufholdt ◽  
Domenica Hamisch ◽  
Rolf Bikker ◽  
Jörg Kudla ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
In Planta ◽  
Protein Protein Interactions ◽  
Gateway Vectors

scholarly journals Highly Modular Protein Micropatterning Sheds Light on the Role of Clathrin-Mediated Endocytosis for the Quantitative Analysis of Protein-Protein Interactions in Live Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 540 ◽  
Author(s):  
Peter Lanzerstorfer ◽  
Ulrike Müller ◽  
Klavdiya Gordiyenko ◽  
Julian Weghuber ◽  
Christof M. Niemeyer
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Spatial Arrangement ◽  
Interaction Partner ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Large Area ◽  
Contact Printing ◽  
Prey Protein ◽  
Protein Micropatterning

Protein micropatterning is a powerful tool for spatial arrangement of transmembrane and intracellular proteins in living cells. The restriction of one interaction partner (the bait, e.g., the receptor) in regular micropatterns within the plasma membrane and the monitoring of the lateral distribution of the bait’s interaction partner (the prey, e.g., the cytosolic downstream molecule) enables the in-depth examination of protein-protein interactions in a live cell context. This study reports on potential pitfalls and difficulties in data interpretation based on the enrichment of clathrin, which is a protein essential for clathrin-mediated receptor endocytosis. Using a highly modular micropatterning approach based on large-area micro-contact printing and streptavidin-biotin-mediated surface functionalization, clathrin was found to form internalization hotspots within the patterned areas, which, potentially, leads to unspecific bait/prey protein co-recruitment. We discuss the consequences of clathrin-coated pit formation on the quantitative analysis of relevant protein-protein interactions, describe controls and strategies to prevent the misinterpretation of data, and show that the use of DNA-based linker systems can lead to the improvement of the technical platform.

Quantitative Analysis of Protein–Protein Interactions by Native Page/Fluorimaging

2005 ◽  
Vol 15 (4) ◽  
pp. 469-473 ◽  
Author(s):  
Kylie M. Wagstaff ◽  
Manisha M. Dias ◽  
Gualtiero Alvisi ◽  
David A. Jans
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Native Page

scholarly journals Development of a Model Protein Interaction Pair as a Benchmarking Tool for the Quantitative Analysis of 2-Site Protein-Protein Interactions

2015 ◽  
Vol 26 (4) ◽  
pp. 125-141 ◽  
Author(s):  
Aaron P. Yamniuk ◽  
John A. Newitt ◽  
Michael L. Doyle ◽  
Fumio Arisaka ◽  
Anthony M. Giannetti ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Model Protein ◽  
Protein Interaction Pair
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