Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants

2015 ◽  
pp. 243-253 ◽  
Author(s):  
Gavin M. George ◽  
Michael E. Ruckle ◽  
James R. Lloyd
Keyword(s):  
Drought Tolerance ◽  
Gene Silencing ◽  
Virus Induced Gene Silencing

scholarly journals Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing

2018 ◽  
Vol 19 (12) ◽  
pp. 4020 ◽  
Author(s):  
Xinbo Wang ◽  
Yanhua Xu ◽  
Jingjing Li ◽  
Yongzhe Ren ◽  
Zhiqiang Wang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Gene Silencing ◽  
Proteomic Analysis ◽  
Crop Yields ◽  
Stress Conditions ◽  
Virus Induced Gene Silencing ◽  
Knock Down ◽  
Comparative Proteomic Analysis ◽  
Related Proteins

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

scholarly journals Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance

2019 ◽  
Vol 52 (1) ◽  
Author(s):  
Xinbo Wang ◽  
Yongzhe Ren ◽  
Jingjing Li ◽  
Zhiqiang Wang ◽  
Zeyu Xin ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Gene Silencing ◽  
Virus Induced Gene Silencing ◽  
Knock Down

scholarly journals Virus-induced gene silencing of SlPYL4 decreases the drought tolerance of tomato

2021 ◽  
Author(s):  
Yue Li ◽  
Xiaochun Zhang ◽  
Jingbin Jiang ◽  
Tingting Zhao ◽  
Xiangyang Xu ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Gene Silencing ◽  
Virus Induced Gene Silencing

scholarly journals Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance

2013 ◽  
Vol 64 (5) ◽  
pp. 1381-1392 ◽  
Author(s):  
Harish Manmathan ◽  
Dale Shaner ◽  
Jacob Snelling ◽  
Ned Tisserat ◽  
Nora Lapitan
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Tolerance ◽  
Gene Silencing ◽  
Virus Induced Gene Silencing

Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (12) ◽  
Author(s):  
Lokanadha Gunupuru ◽  
Shahin Ali ◽  
Fiona Doohan ◽  
Steven Scofield
Keyword(s):  
Gene Silencing ◽  
Virus Induced Gene Silencing ◽  
Barley Seedling

scholarly journals Cucumber mosaic virus-induced gene silencing in banana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuh Tzean ◽  
Ming-Chi Lee ◽  
Hsiao-Hsuan Jan ◽  
Yi-Shu Chiu ◽  
Tsui-Chin Tu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Induced Gene Silencing

scholarly journals A cassava common mosaic virus vector for virus-induced gene silencing in cassava

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Decai Tuo ◽  
Peng Zhou ◽  
Pu Yan ◽  
Hongguang Cui ◽  
Yang Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Gene Function ◽  
Viral Vector ◽  
Function Analysis ◽  
Systemic Infection ◽  
Cdna Clones ◽  
Virus Induced Gene Silencing ◽  
Efficient Gene ◽  
Gene Function Analysis

Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.

scholarly journals Virus-induced gene silencing as a tool for analysis of gene functions in plants

Uirusu ◽  
2010 ◽  
Vol 60 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Noriko YAMAGISHI ◽  
Nobuyuki YOSHIKAWA
Keyword(s):  
Gene Silencing ◽  
Virus Induced Gene Silencing ◽  
Gene Functions
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