Quantitation of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single-Molecule PCR

2016 ◽  
pp. 33-46 ◽  
Author(s):  
Yevgenya Kraytsberg ◽  
Xinhong Guo ◽  
Saisai Tao ◽  
Alexandra Kuznetsov ◽  
Catherine MacLean ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Long Range ◽  
Single Molecule ◽  
Restriction Digestion ◽  
Dna Deletions

ERRATUM TO: Quantitation of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single-Molecule PCR

2016 ◽  
pp. E1-E1
Author(s):  
Yevgenya Kraytsberg ◽  
Xinhong Guo ◽  
Saisai Tao ◽  
Alexandra Kuznetsov ◽  
Catherine MacLean ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Long Range ◽  
Single Molecule ◽  
Restriction Digestion ◽  
Dna Deletions

Increased Mitochondrial DNA Deletions in Substantia Nigra Dopamine Neurons of the Aged Rat

2015 ◽  
Vol 7 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Gemma M. Parkinson ◽  
Christopher V. Dayas ◽  
Doug W. Smith
Keyword(s):  
Mitochondrial Dna ◽  
Substantia Nigra ◽  
Dopamine Neurons ◽  
Aged Rat ◽  
Dna Deletions

Mitochondrial DNA deletions in the peripheral blood of workers at the Mayak PA who were exposed to long-term combined effects of external γ- and internal α-radiation

BIOPHYSICS ◽  
2016 ◽  
Vol 61 (6) ◽  
pp. 1026-1032
Author(s):  
L. V. Malakhova ◽  
M. G. Lomaeva ◽  
M. L. Zakharova ◽  
E. N. Kirillova ◽  
S. N. Sokolova ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Peripheral Blood ◽  
Combined Effects ◽  
Dna Deletions

Unusual pattern of mitochondrial DNA deletions in skeletal muscle of an adult human with chronic fatigue syndrome

1995 ◽  
Vol 4 (4) ◽  
pp. 751-754 ◽  
Author(s):  
Chunfang Zhang ◽  
Alessandra Baumer ◽  
Ian R. Mackay ◽  
Anthony W. Linnane ◽  
Phillip Nagley
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Dna ◽  
Chronic Fatigue Syndrome ◽  
Chronic Fatigue ◽  
Unusual Pattern ◽  
Adult Human ◽  
Fatigue Syndrome ◽  
Dna Deletions

Strictly linear trinuclear Dy–Ca/Mg–Dy single-molecule magnets: the impact of long-range f–f ferromagnetic interactions on suppressing quantum tunnelling of magnetization leading to slow magnetic relaxation

2017 ◽  
Vol 46 (25) ◽  
pp. 8259-8268 ◽  
Author(s):  
Wan-Ying Zhang ◽  
Yong-Mei Tian ◽  
Hong-Feng Li ◽  
Peng Chen ◽  
Yi-Quan Zhang ◽  
...  
Keyword(s):  
Long Range ◽  
Single Molecule ◽  
Magnetic Relaxation ◽  
Single Molecule Magnets ◽  
Quantum Tunnelling ◽  
Trinuclear Complexes ◽  
Slow Magnetic Relaxation ◽  
Ferromagnetic Interactions ◽  
The Impact

A series of linear trinuclear complexes Ln2M(OQ)8 [Ln(iii) = Dy and Er, M(ii) = Ca and Mg] were structurally and magnetically investigated.

scholarly journals No mitochondrial DNA deletions but more D-loop point mutations in repeated pregnancy loss

2010 ◽  
Vol 27 (11) ◽  
pp. 641-648 ◽  
Author(s):  
Seyed Mohammad Seyedhassani ◽  
Massoud Houshmand ◽  
Seyed Mehdi Kalantar ◽  
Glayol Modabber ◽  
Abbas Aflatoonian
Keyword(s):  
Mitochondrial Dna ◽  
Pregnancy Loss ◽  
Point Mutations ◽  
Dna Deletions ◽  
D Loop ◽  
Repeated Pregnancy Loss

scholarly journals Measuring Fate and Rate of Single-Molecule Competition of Amplification and Restriction Digestion, and Its Use for Rapid Genotyping Tested with Hepatitis C Viral RNA

2014 ◽  
Vol 126 (31) ◽  
pp. 8226-8230 ◽  
Author(s):  
Bing Sun ◽  
Jesus Rodriguez-Manzano ◽  
David A. Selck ◽  
Eugenia Khorosheva ◽  
Mikhail A. Karymov ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Single Molecule ◽  
Viral Rna ◽  
Restriction Digestion

scholarly journals Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Jason C Bell ◽  
Bian Liu ◽  
Stephen C Kowalczykowski
Keyword(s):  
Long Range ◽  
Single Molecule ◽  
Magnetic Tweezers ◽  
Intramolecular Interactions ◽  
Ssdna Binding ◽  
Ssdna Binding Protein ◽  
Intramolecular Condensation ◽  
Nucleoprotein Complexes ◽  
Ssdna Binding Proteins ◽  
Dna Maintenance

Escherichia coli single-stranded DNA (ssDNA) binding protein (SSB) is the defining bacterial member of ssDNA binding proteins essential for DNA maintenance. SSB binds ssDNA with a variable footprint of ∼30–70 nucleotides, reflecting partial or full wrapping of ssDNA around a tetramer of SSB. We directly imaged single molecules of SSB-coated ssDNA using total internal reflection fluorescence (TIRF) microscopy and observed intramolecular condensation of nucleoprotein complexes exceeding expectations based on simple wrapping transitions. We further examined this unexpected property by single-molecule force spectroscopy using magnetic tweezers. In conditions favoring complete wrapping, SSB engages in long-range reversible intramolecular interactions resulting in condensation of the SSB-ssDNA complex. RecO and RecOR, which interact with SSB, further condensed the complex. Our data support the idea that RecOR--and possibly other SSB-interacting proteins—function(s) in part to alter long-range, macroscopic interactions between or throughout nucleoprotein complexes by microscopically altering wrapping and bridging distant sites.

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