A Real-Time Cell Analyzing Assay for Identification of Novel Antiviral Compounds against Chikungunya Virus

2016 ◽  
pp. 255-262 ◽  
Author(s):  
Keivan Zandi
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Antiviral Compounds

scholarly journals Complete Coding Sequence of a Case of Chikungunya Virus Imported into Australia

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Bixing Huang ◽  
Alyssa T. Pyke ◽  
Jamie McMahon ◽  
David Warrilow
Keyword(s):  
Phylogenetic Analysis ◽  
Virus Infection ◽  
Real Time ◽  
South African ◽  
Genome Sequencing ◽  
Reverse Transcription ◽  
Chikungunya Virus ◽  
East Central ◽  
Reverse Transcription Pcr ◽  
Central South

ABSTRACT A case of chikungunya virus infection was imported from India into Australia in late 2016. Infection was diagnosed by real-time reverse transcription-PCR and confirmed by culture isolation and genome sequencing. Phylogenetic analysis of the genome sequence indicated that the virus grouped with the east/central/south African genotype.

scholarly journals Update Management of Chikungunya

2017 ◽  
Vol 12 (2) ◽  
pp. 82-85
Author(s):  
Khan Mohammad Arif
Keyword(s):  
Viral Infection ◽  
Real Time ◽  
Real Time Pcr ◽  
Rainy Season ◽  
Joint Pain ◽  
Chikungunya Virus ◽  
Striking Feature ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
Artificial Water

Chikungunya is a viral infection first detected after an outbreak in Tanzania in 1952. Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that belongs to the Togaviridae family. Incidence increases in rainy season. Exact pathogenesis is not clearly understood. Fever and arthralgia/arthritis is the striking feature of Chikungunya fever1. Few patient may develop neurological and other complication. Joint pain may persist for several years. Investigations for confirmation are Real-time PCR, Virus specific IgM antibodies and IgG antibodies. Treatments are supportive. Most patients recover completely. Death is very rare. Reducing natural & artificial water filled container habitats is the principal step of prevention.Faridpur Med. Coll. J. Jul 2017;12(2): 82-85

Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay

2016 ◽  
pp. 105-117 ◽  
Author(s):  
Seok Mui Wang ◽  
Ummul Haninah Ali ◽  
Shamala Devi Sekaran ◽  
Ravindran Thayan
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Detection And Quantification

scholarly journals Chikungunya virus (CHIKV) infection: Analytical performance of real-time NASBA assay

2010 ◽  
Vol 14 ◽  
pp. e51
Author(s):  
G. Rossini ◽  
F. Cavrini ◽  
P. Gaibani ◽  
A. Pierro ◽  
M.P. Landini ◽  
...  
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Analytical Performance ◽  
Chikv Infection ◽  
Nasba Assay

scholarly journals Report of Chikungunya Virus in Wild Populations of Aedes aegypti in Guerrero State, Mexico

2018 ◽  
Vol 34 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Gustavo Ponce-García ◽  
Adriana E. Flores-Suarez ◽  
Karina Villanueva-Segura ◽  
Martha Lopez-Rodriguez ◽  
Felipe Dzul ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Chikungunya Virus ◽  
Wild Populations ◽  
Chain Reaction ◽  
Male And Female ◽  
San Marcos ◽  
Polymerase Chain

ABSTRACT We detected vertical transmission of chikungunya virus (CHIKV) in wild populations of Aedes aegypti from San Marcos, Guerrero, Mexico, with real-time reverse transcriptase–polymerase chain reaction. A total of 20 pools (1–11 specimens/pool) of larvae, male, and female mosquitoes were tested. We report the detection of CHIKV in 2 of 11 larval pools, 4 of 5 male pools, and 1 of 4 female pools, from field-collected mosquitoes.

scholarly journals Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: Efficacy of decitabine and valganciclovir alone or in combination

2020 ◽  
Vol 183 ◽  
pp. 104931 ◽  
Author(s):  
Côme Thieulent ◽  
Erika S. Hue ◽  
Gabrielle Sutton ◽  
Christine Fortier ◽  
Patrick Dallemagne ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Assay ◽  
Antiviral Compounds ◽  
Equid Herpesvirus ◽  
Herpesvirus 1

scholarly journals Evaluation of real-time nucleic acid sequence-based amplification for detection of Chikungunya virus in clinical samples

2009 ◽  
Vol 58 (9) ◽  
pp. 1168-1172 ◽  
Author(s):  
J.-N. Telles ◽  
K. Le Roux ◽  
P. Grivard ◽  
G. Vernet ◽  
A. Michault
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Chikungunya Virus ◽  
False Negative ◽  
Nucleic Acid Sequence ◽  
Clinical Samples ◽  
Acid Extraction ◽  
Plasma Samples ◽  
Rt Pcr ◽  
Nucleic Acid Extraction

The Chikungunya virus (CHIKV) is a member of the genus Alphavirus that is transmitted to humans by Aedes mosquitoes. In 2005 and 2006, the Indian Ocean island of La Réunion was hit with an unprecedented CHIKV fever outbreak that infected 300 000 people. In the present study, we describe the evaluation of real-time nucleic acid sequence-based amplification (RT-NASBA) for the detection of CHIKV in clinical samples. A co-extracted and co-amplified chimerical CHIKV RNA sequence was used as an internal control to eliminate false-negative results. The detection threshold of the assay was determined from quantified CHIKV-positive plasma, and estimated to be 200 copies per NASBA reaction. The specificity of the assay was determined using blast analyses and non-cross-reactivity using an O'nyong-nyong virus culture and 250 CHIKV RT-PCR-negative plasma samples. A 100 % specificity was found and no invalid result was obtained, showing the good quality of the nucleic acid extraction. The assay was then evaluated using 252 CHIKV-positive RT-PCR plasma samples. The samples were all tested positive, including those with low viral load. This evaluation showed that the RT-NASBA is a rapid (5 h from sample nucleic acid extraction to detection), sensitive, specific and reliable method for the routine diagnosis of CHIKV in clinical samples.

scholarly journals Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro

2004 ◽  
Vol 63 (3) ◽  
pp. 209-215 ◽  
Author(s):  
S GUNTHER ◽  
M ASPER ◽  
C ROSER ◽  
L LUNA ◽  
C DROSTEN ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Ebola Virus ◽  
Lassa Virus ◽  
Sars Coronavirus ◽  
Antiviral Compounds
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