scholarly journals A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis

2016 ◽  
pp. 141-149 ◽  
Author(s):  
Jinbo Fan ◽  
Hui Jin ◽  
Hong-Guo Yu
Keyword(s):  
Gene Regulation ◽  
Budding Yeast ◽  
Reporter Assay ◽  
Dual Color ◽  
Yeast Meiosis

scholarly journals Programmed ER fragmentation drives selective ER inheritance and degradation in budding yeast meiosis

2021 ◽  
Author(s):  
George M. Otto ◽  
Tia Cheunkarndee ◽  
Jessica M. Leslie ◽  
Gloria A. Brar
Keyword(s):  
Plasma Membrane ◽  
Cell Differentiation ◽  
Budding Yeast ◽  
Developmentally Regulated ◽  
Selective Degradation ◽  
Cell Plasma ◽  
Membrane Tethering ◽  
Membrane Bound ◽  
Yeast Meiosis ◽  
And Function

AbstractThe endoplasmic reticulum (ER) is a membrane-bound organelle with diverse, essential functions that rely on the maintenance of membrane shape and distribution within cells. ER structure and function are remodeled in response to changes in cellular demand, such as the presence of external stressors or the onset of cell differentiation, but mechanisms controlling ER remodeling during cell differentiation are not well understood. Here, we describe a series of developmentally regulated changes in ER morphology and composition during budding yeast meiosis, a conserved differentiation program that gives rise to gametes. During meiosis, the cortical ER undergoes fragmentation before collapsing away from the plasma membrane at anaphase II. This programmed collapse depends on the meiotic transcription factor Ndt80, conserved ER membrane structuring proteins Lnp1 and reticulons, and the actin cytoskeleton. A subset of ER is retained at the mother cell plasma membrane and excluded from gamete cells via the action of ER-plasma membrane tethering proteins. ER remodeling is coupled to ER degradation by selective autophagy, which is regulated by the developmentally timed expression of the autophagy receptor Atg40. Autophagy relies on ER collapse, as artificially targeting ER proteins to the cortically retained ER pool prevents their degradation. Thus, developmentally programmed changes in ER morphology determine the selective degradation or inheritance of ER subdomains by gametes.

scholarly journals sRNA/L1 retrotransposition: using siRNAs and miRNAs to expand the applications of the cell culture-based LINE-1 retrotransposition assay

2020 ◽  
Vol 375 (1795) ◽  
pp. 20190346 ◽  
Author(s):  
Pablo Tristan-Ramos ◽  
Santiago Morell ◽  
Laura Sanchez ◽  
Belen Toledo ◽  
Jose L. Garcia-Perez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Gene Regulation ◽  
Small Rna ◽  
Host Factors ◽  
Fanconi Anaemia ◽  
Reporter Assay ◽  
Long Interspersed Elements ◽  
Essential Tool ◽  
Conventional Cell

The cell culture-based retrotransposition reporter assay has been (and is) an essential tool for the study of vertebrate Long INterspersed Elements (LINEs). Developed more than 20 years ago, this assay has been instrumental in characterizing the role of LINE-encoded proteins in retrotransposition, understanding how ribonucleoprotein particles are formed, how host factors regulate LINE mobilization, etc. Moreover, variations of the conventional assay have been developed to investigate the biology of other currently active human retrotransposons, such as Alu and SVA. Here, we describe a protocol that allows combination of the conventional cell culture-based LINE-1 retrotransposition reporter assay with short interfering RNAs (siRNAs) and microRNA (miRNAs) mimics or inhibitors, which has allowed us to uncover specific miRNAs and host factors that regulate retrotransposition. The protocol described here is highly reproducible, quantitative, robust and flexible, and allows the study of several small RNA classes and various retrotransposons. To illustrate its utility, here we show that siRNAs to Fanconi anaemia proteins (FANC-A and FANC-C) and an inhibitor of miRNA-20 upregulate and downregulate human L1 retrotransposition, respectively. This article is part of a discussion meeting issue ‘Crossroads between transposons and gene regulation’.

scholarly journals Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis

2008 ◽  
Vol 22 (19) ◽  
pp. 2627-2632 ◽  
Author(s):  
A. Sourirajan ◽  
M. Lichten
Keyword(s):  
Budding Yeast ◽  
Yeast Meiosis

scholarly journals How to halve ploidy: lessons from budding yeast meiosis

2012 ◽  
Vol 69 (18) ◽  
pp. 3037-3051 ◽  
Author(s):  
Gary William Kerr ◽  
Sourav Sarkar ◽  
Prakash Arumugam
Keyword(s):  
Budding Yeast ◽  
Yeast Meiosis

scholarly journals Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis

PLoS Genetics ◽  
2015 ◽  
Vol 11 (6) ◽  
pp. e1005335 ◽  
Author(s):  
Karen Voelkel-Meiman ◽  
Cassandra Johnston ◽  
Yashna Thappeta ◽  
Vijayalakshmi V. Subramanian ◽  
Andreas Hochwagen ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Yeast Meiosis

scholarly journals Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis

PLoS Genetics ◽  
2017 ◽  
Vol 13 (6) ◽  
pp. e1006830 ◽  
Author(s):  
Ping Li ◽  
Hui Jin ◽  
Bailey A. Koch ◽  
Rebecca L. Abblett ◽  
Xuemei Han ◽  
...  
Keyword(s):  
Budding Yeast ◽  
The Sun ◽  
N Terminus ◽  
Domain Protein ◽  
Yeast Meiosis ◽  
Sun Domain
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