Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality

2017 ◽  
pp. 197-210
Author(s):  
Ario de Marco
Keyword(s):  
Recombinant Protein ◽  
Functional Quality

Effectiveness of zona pellucida protein ZPB as an immunocontraceptive antigen

Reproduction ◽  
2000 ◽  
pp. 19-32 ◽  
Author(s):  
ML Martinez ◽  
JD Harris
Keyword(s):  
Menstrual Cycle ◽  
Recombinant Protein ◽  
Antibody Titre ◽  
Zona Pellucida ◽  
Protein A ◽  
Papio Cynocephalus ◽  
Cynomolgus Monkeys ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle

Immunization of female mammals with native zona pellucida (ZP) proteins is known to cause infertility. Since each human ZP protein is now available as a purified recombinant protein, is it possible to compare the immunocontraceptive potential of each ZP protein. A breeding study was conducted in cynomolgus monkeys (Macaca fasicularis) after immunization with recombinant human ZP (rhZP) proteins (ZPA, ZPB, ZPC) separately and in combinations. This study demonstrated that immunization with recombinant human ZPB (rhZPB) protein caused cynomolgus monkeys to become infertile for 9-35 months. A second study was conducted in baboons (Papio cynocephalus), which yielded a similar result. The baboons immunized with rhZPB became infertile for 9 to > 20 months. During the time of maximum antibody titre, some animals experienced disruption of the menstrual cycle, but eventually all of the animals resumed normal menstrual cycles. Control animals and animals immunized with other rhZP proteins all became pregnant before any of the rhZPB-treated animals. This is the first study in which a recombinant ZP protein has consistently induced infertility in a primate without permanent disruption of the normal menstrual cycle.

Use of Lectins for Analysis of Glycans in Recombinant Protein Biopharmaceuticals

2018 ◽  
pp. 707-709
Author(s):  
V. Monakhova ◽  
M. Sushkin ◽  
N. Pigareva ◽  
A. Simbirtsev
Keyword(s):  
Recombinant Protein

Immunoenhancement of Recombinant Neisseria meningitides PorB Protein on Porcine Circovirus Type 2 and Mycoplasma hyopneumoniae Genetically Engineered Vaccines

2019 ◽  
Vol 26 (10) ◽  
pp. 776-784
Author(s):  
Rui Yang ◽  
Yu Tao ◽  
Gaojian Li ◽  
Jian Chen ◽  
Jianhong Shu ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Mycoplasma Hyopneumoniae ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Genetically Engineered ◽  
Porcine Circovirus ◽  
Practical Applications ◽  
Neisseria Meningitides ◽  
Short Period

Background:Porcine circovirus and Mycoplasma hyopneumoniae can cause respiratory diseases in pigs, which cause serious economic loss in the worldwide pig industry. Currently, these infections are mainly prevented and controlled by vaccination. The new vaccines on the market are mainly composed of subunits and inactivated vaccines but usually have lower antigenicity than traditional live vaccines. Thus, there is an increasing need to develop new adjuvants that can cause rapid and long-lasting immunity to enhance the antigenic efficacy for vaccines. Studies have shown that meningococcal porin PorB can act as a ligand to combine with Toll-like receptors to activate the production of immunological projections and act as a vaccine immunological adjuvant.Objective:In this article, we expressed and purified the recombinant PorB protein and verified its immunogenicity against porcine circovirus type 2 and Mycoplasma hyopneumoniae genetically engineered vaccine.Methods:In this article, we used prokaryotic expression to express and purify recombinant PorB protein, four different concentrations of PorB protein, Freund's adjuvant with two genetically engineered vaccines were combined with subcutaneous immunization of mice.Results:Our study shows that the appropriate dose of the recombinant protein PorB can enhance the levels of humoral and cellular responses induced by two genetically engineered vaccines in a short period of time in mice. The PorB adjuvant group may cause statistically higher antibody titers for both genetically engineered vaccines compared to Freund's commercial adjuvant (P<0.001).Conclusion:The recombinant protein PorB may be a good candidate adjuvant for improving the protective effect of vaccines against porcine circovirus type 2 and Mycoplasma hyopneumoniae, and the protein can be used for future practical applications.

Nutritional and Bioactive Components of Carioca Common Bean (Phaseolus vulgaris L.) Tempeh and Yellow Soybean (Glycine max L.) Tempeh

2020 ◽  
Vol 16 (5) ◽  
pp. 768-775
Author(s):  
Juliana A.C. Bento ◽  
Priscila Z. Bassinello ◽  
Aline O. Colombo ◽  
Rayane J. Vital ◽  
Rosângela N. Carvalho
Keyword(s):  
Antioxidant Capacity ◽  
Common Bean ◽  
Chemical Properties ◽  
Common Beans ◽  
Bioactive Components ◽  
High Fiber ◽  
Functional Quality ◽  
Dpph Method ◽  
Glycine Max L ◽  
Interesting Alternative

Background: Tempeh, a product of Indonesian origin based on fermented soybeans, is present in naturalistic diets and has been gaining popularity in other cultures and diets due to the convenience for food preparation and use, aside from the nutritional and functional quality. Methods: In this work, tempehs were produced from common bean, carioca grain type (CBT) aged and peeled, and in combination with yellow soybean (CBST: 50:50). Products were characterized based on nutritional quality (proximate composition, fiber, mineral, vitamin B12, energetic value), physical-chemical properties (pH, acidity, moisture, water activity), functional characteristics (antioxidant capacity), sensorial aspects (texture and color) and antinutrient content. Data from beanbased tempehs and the commercially purchased traditional soybean tempeh (ST) were statistically evaluated and the means compared by Tukey test. Results: Although CBT was found nutritionally inferior to ST, its contribution on protein intake was noteworthy and, unlike ST, it presented high fiber content and low caloric value. P, K, Mg, Cu and Mo levels meet daily requirements for adults. CBT showed higher antioxidant capacity by DPPH method and was significantly more soft and elastic and less sticky and gummy when compared to ST, and L, a* and b* CBT color parameters were more pronounced than ST. Conclusion: It can thus be concluded that tempeh based on carioca common beans can be an interesting alternative for stored grains, with good sensorial and functional properties and making an important nutritional contribution to the diet.

Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.

1987 ◽  
Vol 61 (11) ◽  
pp. 3645-3647 ◽  
Author(s):  
R Ostermayr ◽  
K von der Helm ◽  
V Gauss-Müller ◽  
E L Winnacker ◽  
F Deinhardt
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Cdna

A Framework for Non-Nominal Visualization and Perceived Quality Evaluation

2011 ◽  
Author(s):  
Ola Wagersten ◽  
Karin Forslund ◽  
Casper Wickman ◽  
Rikard So¨derberg
Keyword(s):  
Industrial Process ◽  
Perceived Quality ◽  
Product Development Process ◽  
Functional Quality ◽  
Quality Aspects ◽  
System Solution ◽  
Comprehensive Framework ◽  
Variation Simulation ◽  
Support Evaluation

Perceived Quality clusters different aspects that influence the customer’s perception of non-functional quality on a product that are perceive through senses. All together those aspects and the harmony between them reflect the producer’s ability to control product parameters and thereby also mirror the functional quality of the product. High Perceived Quality cannot be added to the product at the end of the developing process. Project prerequisites, system solution, factory capability etc. are criterion to succeed. Therefore, it is important to be able to evaluate Perceived Quality early in the process when product system solutions and architecture are defined, although data maturity is low. This paper presents a comprehensive framework to manage and support evaluation of Perceived Quality aspects in a product development process. The framework is based on an industrial process in combination with recent research within the field. The framework focuses on activities that can be performed at different stages in the developing process based on maturity of the CAD or styling data. For example, if the styling data is divided into different components by split-lines it has reached higher level of maturity then styling data that not has been divided. Consequently, the choice of applied method is based on data maturity, regardless phase in the developing process. The framework contains methods based on several different simulation and analysis techniques. Design methods, Computer-Aided Tolerancing and FEA based non-rigid variation simulation are represented in the framework.

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