Effectiveness of zona pellucida protein ZPB as an immunocontraceptive antigen

Reproduction ◽  
2000 ◽  
pp. 19-32 ◽  
Author(s):  
ML Martinez ◽  
JD Harris
Keyword(s):  
Menstrual Cycle ◽  
Recombinant Protein ◽  
Antibody Titre ◽  
Zona Pellucida ◽  
Protein A ◽  
Papio Cynocephalus ◽  
Cynomolgus Monkeys ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle

Immunization of female mammals with native zona pellucida (ZP) proteins is known to cause infertility. Since each human ZP protein is now available as a purified recombinant protein, is it possible to compare the immunocontraceptive potential of each ZP protein. A breeding study was conducted in cynomolgus monkeys (Macaca fasicularis) after immunization with recombinant human ZP (rhZP) proteins (ZPA, ZPB, ZPC) separately and in combinations. This study demonstrated that immunization with recombinant human ZPB (rhZPB) protein caused cynomolgus monkeys to become infertile for 9-35 months. A second study was conducted in baboons (Papio cynocephalus), which yielded a similar result. The baboons immunized with rhZPB became infertile for 9 to > 20 months. During the time of maximum antibody titre, some animals experienced disruption of the menstrual cycle, but eventually all of the animals resumed normal menstrual cycles. Control animals and animals immunized with other rhZP proteins all became pregnant before any of the rhZPB-treated animals. This is the first study in which a recombinant ZP protein has consistently induced infertility in a primate without permanent disruption of the normal menstrual cycle.

Does the menstrual cycle influence the sensitivity of vagally mediated baroreflexes?

2002 ◽  
Vol 102 (6) ◽  
pp. 639-644 ◽  
Author(s):  
William H. COOKE ◽  
David A. LUDWIG ◽  
Paul S. HOGG ◽  
Dwain L. ECKBERG ◽  
Victor A. CONVERTINO
Keyword(s):  
Menstrual Cycle ◽  
Young Women ◽  
Regulatory Mechanisms ◽  
Physiological Changes ◽  
Menstrual Cycles ◽  
Time Points ◽  
Normal Menstrual Cycle ◽  
Cardiac Regulation ◽  
Neck Pressure ◽  
Noradrenaline Levels

The menstrual cycle provokes several physiological changes that could influence autonomic regulatory mechanisms. We studied the carotid-cardiac baroreflex in ten healthy young women on four occasions over the course of their menstrual cycles (days 0-8, 9-14, 15-20 and 21-25). We drew blood during each session for analysis of oestrogen, progesterone and noradrenaline (norepinephrine) levels, and assessed carotid-cardiac baroreflex function by analysing R-R interval responses to graded neck pressure sequences. Oestrogen levels followed a classical two-peak (cubic) response, with elevated levels on days 9-14 and 21-25 compared with days 0-8 and 15-20 (P =0.0032), while progesterone levels increased exponentially from days 9-14 to days 21-25 (P = 0.0063). Noradrenaline levels increased from an average of 137pg/ml during the first three measurement periods to 199pg/ml during days 21-25 (P = 0.0456). Carotid-cardiac baroreflex gain and operational point were not statistically different at any of the time points during the menstrual cycle (P⩾0.18). These findings are consistent with the notion that beat-to-beat vagal-cardiac regulation does not change over the course of the normal menstrual cycle.

APC resistance during the normal menstrual cycle

2007 ◽  
Vol 98 (12) ◽  
pp. 1246-1251 ◽  
Author(s):  
Angela Silveira ◽  
Stella Thomassen ◽  
Jacob Odeberg ◽  
Anders Hamsten ◽  
Jan Rosing ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Protein C ◽  
Activated Protein C ◽  
Serum Levels ◽  
Serum Estradiol ◽  
Menstrual Cycles ◽  
Apc Resistance ◽  
Exogenous Estrogen ◽  
Normal Menstrual Cycle ◽  
Estradiol Concentration

SummaryIncreased serum levels of endogenous as well as exogenous estrogen are regarded to be responsible for acquired activated protein C (APC) resistance. It was the objective of this study to evaluate whether the physiological increase in serum estradiol concentration during the normal menstrual cycle affects the individual’s sensitivity to APC. Seventy-two women with normal menstrual cycles were included in the study. Blood samples for analysis of estradiol (E2), progesterone (P4) and APC resistance were drawn at two time points of the menstrual cycle (day 3–5 and day 22–25). Two methods of measuring APC resistance were used: the activated partial thromboplastin time (aPTT)-based assay and the endogenous thrombin potential (ETP)-based APC resistance test. Independent of the method used, no changes in APC resistance were found, even though the E2 concentration increased significantly between the two menstrual phases. No correlations between E2 levels and APC resistance, P4 levels and APC resistance or changes in E2 concentrations and changes in APC resistance were detected. Ten women were carriers of the factor VLeiden mutation. Their baseline APC resistance was increased, but their response to elevated E2 during the menstrual cycle did not differ from that of non-carriers. In conclusion, our observations suggest that physiological differences in serum levels of estradiol and progesterone between the early follicular and the luteal phase in a normal menstrual cycle do not have any significant impact on the individual’s sensitivity to APC.

OVULATION INHIBITION WITH HUMAN CHORIONIC GONADOTROPHIN

1975 ◽  
Vol 78 (2) ◽  
pp. 332-342 ◽  
Author(s):  
F. Friedrich ◽  
P. Kemeter ◽  
H. Salzer ◽  
G. Breitenecker
Keyword(s):  
Menstrual Cycle ◽  
Body Temperature ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Basal Body Temperature ◽  
Menstrual Cycles ◽  
Ovulation Inhibition ◽  
Normal Menstrual Cycle ◽  
Theca Interna ◽  
Secretory Endometrium

ABSTRACT Eight women with regular menstrual cycles were treated daily during 9 cycles with HCG (Human Chorionic Gonadotrophin) 3000 or 5000 IU daily for a period of 4–7 days. This treatment was started between the 1st and the 6th day after the onset of menstruation. Control of the treatment cycles was performed by basal body temperature, pregnanediol serial estimations, endometrial biopsies and in addition in 5 treatment cycles by radio-immunological assay of oestradiol-17β (Oe2), progesterone, LH and FSH from the serum at intervals of 1 to 3 days. In 6 of these cycles where treatment started on the 4th day or later, ovulation was inhibited (2 cycles) or postponed (4 cycles) to the 24th–46th day. In these 6 treatment cycles the progesterone and pregnanediol increase during HCG treatment was poor or absent. The typical Oe2 increase of the normal menstrual cycle was impaired. In the 3 remaining cycles where treatment was started on the 1st, 2nd and 4th day, we observed during HCG treatment increases in Oe2 and progesterone serum values similar to that found during corpus luteum activity, and menstruation from a secretory endometrium between the 13th–19th day of the cycle. The histologically examined ovaries of one woman who was treated with HCG from the 2nd to the 6th day of the cycle showed distinct Iuteinization of the theca interna of all tertiary follicles and a beginning degeneration of the granulosa. These findings give support to the hypothesis that the luteinization of the theca interna leads to degeneration of the tertiary follicles thereby causing ovulation inhibition or postponement of ovulation.

Estradiol and progesterone levels in the endometrium and the reproductive tract luminal washing during the pre-implantation stages of gestation in the rhesus monkey

1989 ◽  
Vol 120 (5) ◽  
pp. 649-654 ◽  
Author(s):  
D. Ghosh ◽  
Jayasree Sengupta
Keyword(s):  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Reproductive Tract ◽  
Sex Steroid ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle ◽  
Ovulatory Period ◽  
Hormonal Milieu ◽  
Estradiol 17Β ◽  
Estradiol Concentration

Abstract. To obtain an understanding of the sex steroid hormonal milieu during early gestation, the concentrations of estradiol-17β and progesterone were measured in endometria and reproductive tract luminal fluids collected during the post-ovulatory period (days 2 to 6) of conception, and of non-mated menstrual cycles of the rhesus monkey (Macaca mulatta). Estradiol content was found to be higher (P< 0.05) in endometria obtained from conception cycles; day-wise analysis revealed higher (P< 0.05) level of tissue estradiol concentration on day 6 of post-ovulation as compared with the normal menstrual cycle. Endometrial progesterone content remained, however, unaltered in both groups of monkeys and thus relatively lower (P< 0.05) endometrial progesterone to estradiol ratios were seen on days 4, 5 and 6 of gestation compared with normal menstrual cycle. There were no significant changes in the profiles and concentrations of estradiol and progesterone in luminal washings. The typical sex steroid hormonal milieu observed in the endometrium during the peri-implantation stage of gestation in conception cycle may be related to the endometrial differentiation towards implantation in the rhesus monkey.

CONCENTRATIONS OF OESTRADIOL-17β AND PROGESTERONE IN THE PERIPHERAL PLASMA OF THE CYNOMOLGUS MONKEY (MACACA FASCICULARIS) IN RELATION TO THE LENGTH OF THE MENSTRUAL CYCLE AND ITS COMPONENT PHASES

1978 ◽  
Vol 79 (1) ◽  
pp. 1-7 ◽  
Author(s):  
A. A. SHAIKH ◽  
R. H. NAQVI ◽  
S. A. SHAIKH
Keyword(s):  
Menstrual Cycle ◽  
Cynomolgus Monkey ◽  
Luteal Phase ◽  
Cycle Length ◽  
Macaca Fascicularis ◽  
Phase Correlation ◽  
Cynomolgus Monkeys ◽  
Menstrual Cycles ◽  
Peripheral Plasma ◽  
The Relationship

Ninety-three female cynomolgus monkeys were monitored throughout 647 menstrual cycles; 93·2% of these cycles were 22–37 days long and were normally distributed, with a mean of 29·2 days. Menstrual bleeding generally lasted for 3–5 days and was not related to the length of the cycle. The levels of progesterone and oestradiol-17β in the plasma were measured during the menstrual cycle in 30 monkeys. The concentration of oestradiol-17β reached a mid-cycle peak on day 11 or 12 of the cycle. The interval between the beginning of the cycle and the oestrogen peak was constant; the interval between the oestrogen peak and the end of the cycle increased or decreased with the cycle length. The level of progesterone began to rise at about the time of the oestrogen peak and remained raised for longer periods as the length of the cycle increased. The length of the menstrual cycle, therefore, appeared to be determined by the duration of the increase in the level of progesterone or by the life of the corpus luteum in the luteal phase. The relationship between the lengths of the cycle and the luteal phase can be defined by the expression: cycle length = 12·6+0·96 × length of luteal phase (correlation coefficient = 0·875).

scholarly journals Incidence and etiologic factors responsible for anovulation in infertility cases

2018 ◽  
Vol 7 (4) ◽  
pp. 1567
Author(s):  
Rajeshwari Singangutti
Keyword(s):  
Menstrual Cycle ◽  
False Positive ◽  
Endometrial Biopsy ◽  
Cervical Mucus ◽  
Common Cause ◽  
Menstrual Cycles ◽  
Ovulatory Dysfunction ◽  
Normal Menstrual Cycle ◽  
Positive Results

Background: Chronic anovulation is a common cause of infertility. Anovulation leads to a long-term problem such as hyperandrogenism. Anovulation usually associated with amenorrhoea about 20% and oligomenorrhoea of women with ovulatory dysfunction. Present study was designed to assess the incidence and etiological factors responsible for anovulation in infertility cases.Methods: Study comprised of one hundred patients attending the outdoor clinic of the department for infertility either primary or secondary with regular and irregular menstrual cycles and patients of secondary amenorrhoea.Results: Among the total cases Polymenorrhoea was seen in 10% of cases, oligomenorrhea in 14% cases, secondary amenorrhoea in 3% and 73% cases had normal menstrual cycle. cervical mucus viscosity in ovulatory group to be low in 84.6% cases, moderate in 8.5% cases and high in 38% cases. In anovulatory cycles findings were reversed with viscosity at 14th day it was moderate in 27.27% and higher in 54.54% cases.Conclusions: Cervical mucus is a good indicator of the cyclical changes in ovarian hormonal as no false positive results were found. Endometrial biopsy is the mainstay in infertility studios as it provides information regarding the ovulating status.

Assessing menstrual cycles with urinary hormone assays

2003 ◽  
Vol 284 (3) ◽  
pp. E521-E530 ◽  
Author(s):  
N. Santoro ◽  
S. L. Crawford ◽  
J. E. Allsworth ◽  
E. B. Gold ◽  
G. A. Greendale ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Detection Algorithm ◽  
Menstrual Cycles ◽  
Threshold Detection ◽  
Methods Of Evaluation ◽  
Multiethnic Cohort ◽  
Aging Women ◽  
Perimenopausal Women ◽  
Normal Menstrual Cycle ◽  
Trained Observers

The Study of Women's Health Across the Nation (SWAN) is a multiethnic cohort study of middle-aged women enrolled at seven US sites. A subset of 848 women completed a substudy in which their urinary gonadotropins and sex steroid metabolites were assessed during one complete menstrual cycle or up to 50 consecutive days. Urine was analyzed for LH, FSH, estrone conjugates (E1c), and pregnanediol glucuronide (Pdg). To prepare for serial analysis of this large, longitudinal database in a population of reproductively aging women, we examined the performance of algorithms designed to identify features of the normal menstrual cycle in midreproductive life. Algorithms were based on existing methods and were compared with a “gold standard” of ratings of trained observers on a subset of 396 cycles from the first collection of Daily Hormone Substudy samples. In evaluating luteal status, overall agreement between and within raters was high. Only 17 of the 396 cycles evaluated were considered indeterminate. Of the 328 cycles rated as containing evidence of luteal activity (ELA), 320 were considered ELA by use of a Pdg threshold detection algorithm. Of 51 cycles that were rated as no evidence of luteal activity, only 2 were identified by this algorithm as ELA. Evaluation of the day of the luteal transition with methods that detected a change in the ratio of E1c to Pdg provided 85–92% agreement for day of the luteal transition within 3 days of the raters. Adding further conditions to the algorithm increased agreement only slightly, by 1–8%. We conclude that reliable, robust, and relatively simple objective methods of evaluation of the probability and timing of ovulation can be used with urinary hormonal assays in early perimenopausal women.

scholarly journals Normal Puberty Knowledge and Adolescent Menstrual Cycles

2019 ◽  
Vol 17 (2) ◽  
pp. 119-133
Author(s):  
C. Ermayani Putriyanti ◽  
Eka Ratnawati
Keyword(s):  
Menstrual Cycle ◽  
Junior High School ◽  
Significant Relationship ◽  
Sources Of Information ◽  
Long Term Effects ◽  
Menstrual Cycles ◽  
Central Java ◽  
Normal Menstrual Cycle ◽  
Knowledge Survey

The degree of understanding of puberty is still very varied. It has an impact on the cleanliness of erroneous menstruation, which will have long-term effects on reproductive health. The study aims to identify adolescent knowledge about normal puberty and menstrual cycles. This research was a quantitative study of observational approach by using the knowledge survey of normal puberty and the menstrual cycle involving 55 students of State Junior High School (SMPN) 1 Parakan, Temanggung, Central Java. The technicality used descriptive statistics. The results of this study revealed that knowledge of the degree of normal paper and the menstrual cycle was still low (21.33%). The main sources of information about puberty and menstruation are thousands (85.5%). Teenagers do not know about the interval between the onset of puberty and menarche (87%), few adolescents understand the first sign of puberty (33%). Most teenagers have understood the normal menstrual cycle correctly (78%). There was no significant relationship between knowledge of the first sign of puberty with respondents' understanding of belief. Meanwhile, there was a significant relationship between menstrual status and knowledge about the menstrual cycle and the number of replacement pads. The results showed that students have a poor understanding of puberty, hence, health education must be given to students and their parents.

Changes in blood pressure during the normal menstrual cycle

1991 ◽  
Vol 81 (s25) ◽  
pp. 515-518 ◽  
Author(s):  
F. P. Dunne ◽  
D. G. Barry ◽  
J. B. Ferriss ◽  
G. Grealy ◽  
D. Murphy
Keyword(s):  
Blood Pressure ◽  
Menstrual Cycle ◽  
Systolic Blood Pressure ◽  
Diastolic Blood Pressure ◽  
Luteal Phase ◽  
Mean Difference ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle ◽  
At Home

1. Changes in blood pressure during the normal menstrual cycle are not well documented, and previous studies have given conflicting results. 2. Thirty normotensive women and ten mildly hypertensive women measured their blood pressure at home each morning for 6 weeks, under standardized conditions, using a UA-751 semi-automatic sphygmomanometer. All had normal menstrual cycles and subjects entered the study at difference phases of the cycle. 3. Blood pressure was higher at the onset of menstruation than at most other phases of the cycle (systolic blood pressure, P < 0.05; diastolic blood pressure, P < 0.001). Adjusted diastolic blood pressure was higher in the follicular than in the luteal phase (mean difference 1.23 mmHg, P < 0.001). Similarly, blood pressure was lower during days 17–26 than during the remainder of the cycle (adjusted mean difference in systolic blood pressure −0.65 mmHg, P = 0.07; adjusted mean difference in diastolic blood pressure − 1.19 mmHg, P < 0.001). 4. Similar patterns were seen in normotensive and hypertensive subjects, and changes in plasma 17β-oestradiol and progesterone concentrations were also similar in the two groups.

SOME OBSERVATIONS ON PREGNANEDIOL EXCRETION DURING THE NORMAL MENSTRUAL CYCLE

1957 ◽  
Vol 24 (3_Suppl) ◽  
pp. S207
Author(s):  
A. Klopper
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
New Method ◽  
Menstrual Bleeding ◽  
Normal Menstrual Cycle ◽  
The Relationship ◽  
New View

Abstract The changes in view on the significance and amount of urinary pregnanediol in the menstrual cycle are reviewed; in particular the effects of the discovery that the adrenals in both sexes normally contribute to the urinary pregnanediol. Pregnanediol excretion during the menstrual cycle was studied by means of a new method of assay (Klopper et al., 1955) and the results applied to present day concepts of the growth and duration of the corpus luteum. The relationship between pregnanediol excretion and ovulation or the onset of menstrual bleeding was studied. A new view is put forward on the influence of age and parity on the production of progesterone by the corpus luteum.

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