Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells

2018 ◽  
pp. 231-259 ◽  
Author(s):  
Shahensha Shaik ◽  
Ram Devireddy
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Human Adipose Tissue

Human Adipose Tissue–Derived Adult Stem Cells Can Lead to Multiorgan Engraftment

2010 ◽  
Vol 42 (5) ◽  
pp. 1849-1856 ◽  
Author(s):  
B. Fang ◽  
Y. Li ◽  
Y. Song ◽  
N. Li ◽  
Y. Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Human Adipose Tissue

scholarly journals Effect of Age and Lipoperoxidation in Rat and Human Adipose Tissue-Derived Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-20
Author(s):  
Mario F. Muñoz ◽  
Sandro Argüelles ◽  
Francesco Marotta ◽  
Mario Barbagallo ◽  
Mercedes Cano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Cell Biology ◽  
Adult Stem Cells ◽  
Elongation Factor ◽  
Human Adipose Tissue ◽  
Wide Range ◽  
Proliferation And Differentiation

A wide range of clinical applications in regenerative medicine were opened decades ago with the discovery of adult stem cells. Highly promising adult stem cells are mesenchymal stem/stromal cells derived from adipose tissue (ADSCs), primarily because of their abundance and accessibility. These cells have multipotent properties and have been used extensively to carry out autologous transplants. However, the biology of these cells is not entirely understood. Among other factors, the regeneration capacity of these cells will depend on both their capacity of proliferation/differentiation and the robustness of the biochemical pathways that allow them to survive under adverse conditions like those found in damaged tissues. The transcription factors, such as Nanog and Sox2, have been described as playing an important role in stem cell proliferation and differentiation. Also, the so-called longevity pathways, in which AMPK and SIRT1 proteins play a crucial role, are essential for cell homeostasis under stressful situations. These pathways act by inhibiting the translation through downregulation of elongation factor-2 (eEF2). In order to deepen knowledge of mesenchymal stem cell biology and which factors are determinant in the final therapeutic output, we evaluate in the present study the levels of all of these proteins in the ADSCs from humans and rats and how these levels are affected by aging and the oxidative environment. Due to the effect of aging and oxidative stress, our results suggest that before performing a cell therapy with ADSCs, several aspects reported in this study such as oxidative stress status and proliferation and differentiation capacity should be assessed on these cells. This would allow us to know the robustness of the transplanted cells and to predict the therapeutic result, especially in elder patients, where probably ADSCs do not carry out their biological functions in an optimal way.

Human adipose tissue stem cells: relevance in the pathophysiology of obesity and metabolic diseases and therapeutic applications

2012 ◽  
Vol 14 ◽  
Author(s):  
Angelo Cignarelli ◽  
Sebastio Perrini ◽  
Romina Ficarella ◽  
Alessandro Peschechera ◽  
Pasquale Nigro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Metabolic Diseases ◽  
Human Adipose Tissue ◽  
Cell Types ◽  
Differentiation Potential ◽  
Multipotent Stem Cells ◽  
Therapeutic Applications ◽  
Metabolic Characteristics

Stem cells are unique cells exhibiting self-renewing properties and the potential to differentiate into multiple specialised cell types. Totipotent or pluripotent stem cells are generally abundant in embryonic or fetal tissues, but the use of discarded embryos as sources of these cells raises challenging ethical problems. Adult stem cells can also differentiate into a wide variety of cell types. In particular, adult adipose tissue contains a pool of abundant and accessible multipotent stem cells, designated as adipose-derived stem cells (ASCs), that are able to replicate as undifferentiated cells, to develop as mature adipocytes and to differentiate into multiple other cell types along the mesenchymal lineage, including chondrocytes, myocytes and osteocytes, and also into cells of endodermal and neuroectodermal origin, including beta-cells and neurons, respectively. An impairment in the differentiation potential and biological functions of ASCs may contribute to the development of obesity and related comorbidities. In this review, we summarise different aspects of the ASCs with special reference to the isolation and characterisation of these cell populations, their relation to the biochemical features of the adipose tissue depot of origin and to the metabolic characteristics of the donor subject and discuss some prospective therapeutic applications.

Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction

Cytotherapy ◽  
2009 ◽  
Vol 11 (7) ◽  
pp. 947-957 ◽  
Author(s):  
Stephan Schreml ◽  
Philipp Babilas ◽  
Sabine Fruth ◽  
Evelyn Orsó ◽  
Gerd Schmitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Human Adipose Tissue

Effects of FGF-2 on human adipose tissue derived adult stem cells morphology and chondrogenesis enhancement in Transwell culture

2012 ◽  
Vol 424 (2) ◽  
pp. 234-238 ◽  
Author(s):  
Azadeh Kabiri ◽  
Ebrahim Esfandiari ◽  
Batool Hashemibeni ◽  
Mohammad Kazemi ◽  
Mohammad Mardani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Human Adipose Tissue ◽  
Transwell Culture

Epithelial differentiation of human adipose tissue-derived adult stem cells

2005 ◽  
Vol 330 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Martin Brzoska ◽  
Helmut Geiger ◽  
Stefan Gauer ◽  
Patrick Baer
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Adult Stem Cells ◽  
Human Adipose Tissue ◽  
Epithelial Differentiation

scholarly journals Adipose-Derived Stem Cells for Future Regenerative System Medicine

2012 ◽  
Vol 4 (2) ◽  
pp. 59
Author(s):  
Yani Lina ◽  
Andi Wijaya
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Adult Stem Cells ◽  
Stromal Vascular Fraction ◽  
Human Adipose Tissue ◽  
Lytic Enzymes ◽  
Stromal Stem Cells

BACKGROUND: The potential use of stem cell-based therapies for repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions for a number of disease. Despite the advances, the availability of stem cells remaining a challenge for both scientist and clinicians in pursuing regenerative medicine. CONTENT: Subcutaneous human adipose tissue is an abundant and accessible cell source for applications in tissue engineering and regenerative medicine. Routinely, the adipose issue is digested with collagenase or related lytic enzymes to release a heterogeneous population for stromal vascular fraction (SVF) cells. The SVF cells can be used directly or can be cultured in plastic ware for selection and expansion of an adherent population known as adipose-derived stromal/stem cells (ASCs). Their potential in the ability to differentiate into adipogenic, osteogenic, chondrogenic and other mesenchymal lineages, as well in their other clinically useful properties, includes stimulation of angiogenesis and suppression of inflammation.SUMMARY: Adipose tissue is now recognized as an accessible, abundant and reliable site for the isolation of adult stem cels suitable for the application of tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models.KEYWORDS: adipose tissue, adult stem cells, regenerative medicine, mesenchymal stem cells

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