Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas

2018 ◽  
pp. 307-317
Author(s):  
Luciana Galetto ◽  
Marta Vallino ◽  
Mahnaz Rashidi ◽  
Cristina Marzachì
Keyword(s):  
Salivary Gland ◽  
Immunofluorescence Assay

scholarly journals Developmentally regulated infectivity of malaria sporozoites for mosquito salivary glands and the vertebrate host.

1992 ◽  
Vol 175 (6) ◽  
pp. 1607-1612 ◽  
Author(s):  
M G Touray ◽  
A Warburg ◽  
A Laughinghouse ◽  
A U Krettli ◽  
L H Miller
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Blood Meal ◽  
Immunofluorescence Assay ◽  
Circumsporozoite Protein ◽  
Vertebrate Host ◽  
Mosquito Vector ◽  
Mosquito Salivary Gland ◽  
Uninfected Female

Sporozoites are an invasive stage of the malaria parasite in both the mosquito vector and the vertebrate host. We developed an in vivo assay for mosquito salivary gland invasion by preparing Plasmodium gallinaceum sporozoites from infected Aedes aegypti mosquitoes under physiological conditions and inoculating them into uninfected female Ae. aegypti. Sporozoites from mature oocysts were isolated from mosquito abdomens 10 or 11 d after an infective blood meal. Salivary gland sporozoites were isolated 13 or 14 d after an infective blood meal. Purified oocyst sporozoites that were inoculated into uninfected female mosquitoes invaded their salivary glands. Using the same assay system, sporozoites derived from salivary glands did not reinvade the salivary glands after inoculation. Conversely, as few as 10 to 50 salivary gland sporozoites induced infection in chickens, while only 2 of 10 chickens inoculated with 5,000 oocyst sporozoites were infected. Both sporozoite populations were found to express a circumsporozoite protein on the sporozoite surface as determined by immunofluorescence assay and circumsporozoite precipitation test using a circumsporozoite protein-specific monoclonal antibody. We conclude that molecules other than this circumsporozoite protein may be responsible for the differential invasion of mosquito salivary glands or infection of the vertebrate host.

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Electron Beam-Induced Mass Loss in Freeze-Dried Tissue and Protein Samples

1985 ◽  
Vol 43 ◽  
pp. 470-471
Author(s):  
M.E. Cantino ◽  
M.K. Goddard ◽  
L.E. Wilkinson ◽  
D.E. Johnson
Keyword(s):  
Electron Beam ◽  
Salivary Gland ◽  
Mass Loss ◽  
Counting Rate ◽  
Dose Dependence ◽  
X Ray ◽  
Large Samples ◽  
Freeze Dried ◽  
Muscle Homogenate ◽  
Large Muscle

Quantification in biological x-ray microanalysis depends on accurate evaluation of mass loss. Although several studies have addressed the problem of electron beam induced mass loss from organic samples (eg., 1,2). uncertainty persists as to the dose dependence, the extent of loss, the elemental constituents affected, and the variation in loss for different materials and tissues. in the work described here, we used x-ray counting rate changes to measure mass loss in albumin (used as a quantification standard), salivary gland, and muscle.In order to measure mass loss at low doses (10-4 coul/cm2 ) large samples were needed. While freeze-dried salivary gland sections of the required dimensions were available, muscle sections of this size were difficult to obtain. To simulate large muscle sections, frog or rat muscle homogenate was injected between formvar films which were then stretched over slot grids and freeze-dried. Albumin samples were prepared by a similar procedure. using a solution of bovine serum albumin in water. Samples were irradiated in the STEM mode of a JEOL 100C.

Sialadenoma papilliferum of the bronchus: rare tracheobronchial tumor of salivary gland type

Pneumologie ◽  
2011 ◽  
Vol 65 (S 01) ◽  
Author(s):  
RF Falkenstern-Ge ◽  
G Ott ◽  
G Friedel ◽  
H Markmann ◽  
J Kalla ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Sialadenoma Papilliferum ◽  
Gland Type

Outcomes of Transoral Robotic Surgery for Parapharyngeal Space Salivary Gland Neoplasms

2015 ◽  
Vol 76 (S 01) ◽  
Author(s):  
Christopher Rassekh ◽  
Gregory Weinstein ◽  
Laurie Loevner ◽  
Ara Chalian ◽  
Bert O'Malley
Keyword(s):  
Salivary Gland ◽  
Robotic Surgery ◽  
Parapharyngeal Space ◽  
Transoral Robotic Surgery ◽  
Salivary Gland Neoplasms

Sonoelastographic modalities in the evaluation of salivary gland disease

2016 ◽  
Vol 37 (S 01) ◽  
Author(s):  
B Hofauer ◽  
N Mansour ◽  
M Bas ◽  
K Stock ◽  
A Knopf
Keyword(s):  
Salivary Gland ◽  
Salivary Gland Disease

Isolation and Structural Charaderization of a Potent Inhibitor of Coagulation Factor Xa from the Leech Haementeria ghilianii

1989 ◽  
Vol 61 (03) ◽  
pp. 437-441 ◽  
Author(s):  
Cindra Condra ◽  
Elka Nutt ◽  
Christopher J Petroski ◽  
Ellen Simpson ◽  
P A Friedman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Salivary Gland ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Western Blot ◽  
Potent Inhibitor ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Sds Page ◽  
Bovine Factor

SummaryThe present work reports the discovery and charactenzation of an anticoagulant protein in the salivary gland of the giant bloodsucking leech, H. ghilianii, which is a specific and potent inhibitor of coagulation factor Xa. The inhibitor, purified to homogeneity, displayed subnanomolar inhibition of bovine factor Xa and had a molecular weight of approximately 15,000 as deduced by denaturing SDS-PAGE. The amino acid sequence of the first 43 residues of the H. ghilianii derived inhibitor displayed a striking homology to antistasin, the recently described subnanomolar inhibitor of factor Xa isolated from the Mexican leech, H. officinalis. Antisera prepared to antistasin cross-reacted with the H. ghilianii protein in Western Blot analysis. These data indicate that the giant Amazonian leech, H. ghilianii, and the smaller Mexican leech, H. officinalrs, have similar proteins which disrupt the normal hemostatic clotting mechanisms in their mammalian host’s blood.

Multimodality Treatment and Survival in Sinonasal Minor Salivary Gland Tumors

2019 ◽  
Author(s):  
Sina Torabi ◽  
Todd Spock ◽  
Bruno Cardoso ◽  
Janet Chao ◽  
R. Manes ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Minor Salivary Gland ◽  
Multimodality Treatment ◽  
Salivary Gland Tumors ◽  
Minor Salivary Gland Tumors
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