scholarly journals Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency

2019 ◽  
pp. 143-152
Author(s):  
Matthew C. Gage
Keyword(s):  
Apoptotic Cell ◽  
Cell Engulfment

scholarly journals Impairment of Apoptotic Cell Engulfment by Pyocyanin, a Toxic Metabolite ofPseudomonas aeruginosa

2008 ◽  
Vol 177 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Stephen M. Bianchi ◽  
Lynne R. Prince ◽  
Kathleen McPhillips ◽  
Lucy Allen ◽  
Helen M. Marriott ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Toxic Metabolite ◽  
Cell Engulfment

scholarly journals Two Alternative Mechanisms That Regulate the Presentation of Apoptotic Cell Engulfment Signal in Caenorhabditis elegans

2007 ◽  
Vol 18 (8) ◽  
pp. 3180-3192 ◽  
Author(s):  
Victor Venegas ◽  
Zheng Zhou
Keyword(s):  
Caenorhabditis Elegans ◽  
Abc Transporter ◽  
Germ Cells ◽  
Mammalian Cells ◽  
Developmental Stages ◽  
Apoptotic Cell ◽  
Somatic Cells ◽  
Apoptotic Cells ◽  
Phospholipid Scramblase ◽  
Cell Engulfment

Phosphatidylserine exposed on the surface of apoptotic mammalian cells is considered an “eat-me” signal that attracts phagocytes. The generality of using phosphatidylserine as a clearance signal for apoptotic cells in animals and the regulation of this event remain uncertain. Using ectopically expressed mouse MFG-E8, a secreted phosphatidylserine-binding protein, we detected specific exposure of phosphatidylserine on the surface of apoptotic cells in Caenorhabditis elegans. Masking the surface phosphatidylserine inhibits apoptotic cell engulfment. CED-7, an ATP-binding cassette (ABC) transporter, is necessary for the efficient exposure of phosphatidylserine on apoptotic somatic cells, and for the recognition of these cells by phagocytic receptor CED-1. Alternatively, phosphatidylserine exposure on apoptotic germ cells is not CED-7 dependent, but instead requires phospholipid scramblase PLSC-1, a homologue of mammalian phospholipid scramblases. Moreover, deleting plsc-1 results in the accumulation of apoptotic germ cells but not apoptotic somatic cells. These observations suggest that phosphatidylserine might be recognized by CED-1 and act as a conserved eat-me signal from nematodes to mammals. Furthermore, the two different biochemical activities used in somatic cells (ABC transporter) and germ cells (phospholipid scramblase) suggest an increased complexity in the regulation of phosphatidylserine presentation in response to apoptotic signals in different tissues and during different developmental stages.

scholarly journals Spatial Control of Draper Receptor Signaling Initiates Apoptotic Cell Engulfment

2017 ◽  
Author(s):  
Adam P. Williamson ◽  
Ronald D. Vale
Keyword(s):  
T Cell Receptor ◽  
Apoptotic Cell ◽  
Cell Receptor ◽  
Apoptotic Cells ◽  
S2 Cells ◽  
Triggering Mechanism ◽  
Spatial Control ◽  
Drosophila S2 Cells ◽  
Similarities And Differences ◽  
Cell Engulfment

AbstractThe engulfment of apoptotic cells is essential for tissue homeostasis and responding to damage. Engulfment is mediated by receptors that recognize ligands exposed on apoptotic cells, such as phosphatidylserine (PS). Here, we convert Drosophila S2 cells into proficient phagocytes by transfecting the Draper engulfment receptor and replacing apoptotic cells with PS-coated beads. We show that PS-ligated Draper forms microclusters that exclude a bulky transmembrane phosphatase and recruit phosphotyrosine binding proteins, revealing a triggering mechanism similar to the T cell receptor (TCR). Analogous to the TCR, Draper’s extracellular domain and PS can be replaced with FRB and FKBP respectively, resulting in a rapamycin-inducible engulfment system. However, in contrast to the TCR, we show that localized signaling at Draper microclusters results in time-dependent depolymerization of actin filaments. Collectively, our results reveal mechanistic similarities and differences between the receptors involved in apoptotic corpse clearance and mammalian immunity and demonstrate that engulfment can be reprogrammed towards non-native targets.Condensed titleSpatial Control of Engulfment Signaling

scholarly journals Phagocytic receptor signaling regulates clathrin and epsin-mediated cytoskeletal remodeling during apoptotic cell engulfment in C. elegans

2013 ◽  
Vol 126 (15) ◽  
pp. e1-e1
Author(s):  
Q. Shen ◽  
B. He ◽  
N. Lu ◽  
B. Conradt ◽  
B. D. Grant ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Receptor Signaling ◽  
C Elegans ◽  
Cytoskeletal Remodeling ◽  
Cell Engulfment

scholarly journals Phagocytic receptor signaling regulates clathrin and epsin-mediated cytoskeletal remodeling during apoptotic cell engulfment in C. elegans

Development ◽  
2013 ◽  
Vol 140 (15) ◽  
pp. 3230-3243 ◽  
Author(s):  
Q. Shen ◽  
B. He ◽  
N. Lu ◽  
B. Conradt ◽  
B. D. Grant ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Receptor Signaling ◽  
C Elegans ◽  
Cytoskeletal Remodeling ◽  
Cell Engulfment

scholarly journals An essential role for calcium flux in phagocytes for apoptotic cell engulfment and the anti-inflammatory response

2009 ◽  
Vol 16 (10) ◽  
pp. 1323-1331 ◽  
Author(s):  
M A Gronski ◽  
J M Kinchen ◽  
I J Juncadella ◽  
N C Franc ◽  
K S Ravichandran
Keyword(s):  
Inflammatory Response ◽  
Essential Role ◽  
Apoptotic Cell ◽  
Calcium Flux ◽  
Anti Inflammatory ◽  
Cell Engulfment
Sign in / Sign up

Export Citation Format

Share Document