siRNA Knockdown of Mammalian zDHHCs and Validation of mRNA Expression by RT-qPCR

2019 ◽  
pp. 151-168 ◽  
Author(s):  
Heather McClafferty ◽  
Michael J. Shipston
Keyword(s):  
Mrna Expression ◽  
Sirna Knockdown

scholarly journals Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1βProduction Depending on TLR4/MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Jing Bi ◽  
Shuang Song ◽  
Liurong Fang ◽  
Dang Wang ◽  
Huiyuan Jing ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Alveolar Macrophages ◽  
Nlrp3 Inflammasome ◽  
Respiratory Syndrome Virus ◽  
Dependent Manner ◽  
Swine Industry ◽  
Downstream Signaling ◽  
Infection Treatment ◽  
Sirna Knockdown ◽  
Myd88 Pathway

Porcine reproductive and respiratory syndrome virus (PRRSV) is anArterivirusthat has been devastating the swine industry worldwide since the late 1980s. Previous studies have reported that PRRSV infection induced the production of IL-1β. However, the cellular sensors and signaling pathways involved in this process have not been elucidated yet. Here, we studied the mechanisms responsible for the production of IL-1βin response to highly pathogenic PRRSV. Upon PRRSV infection of primary porcine alveolar macrophages, both mRNA expression and secretion of IL-1βwere significantly increased in a time- and dose-dependent manner. We also investigated the role of several pattern-recognition receptors and adaptor molecules in this response and showed that the TLR4/MyD88 pathway and its downstream signaling molecules, NF-κB, ERK1/2, and p38 MAPKs, were involved in IL-1βproduction during PRRSV infection. Treatment with specific inhibitors or siRNA knockdown assays demonstrated that components of the NLRP3 inflammasome were crucial for IL-1βsecretion but not for IL-1βmRNA expression. Furthermore, TLR4/MyD88/NF-κB signaling pathway was involved in PRRSV-induced expression of NLRP3 inflammasome components. Together, our results deciphered the pathways leading from recognition of PRRSV to the production and release of IL-1β, providing a deeper knowledge of the mechanisms of PRRSV-induced inflammation responses.

Bestatin increases mRNA expression of aminopeptidase A

1997 ◽  
Vol 56 (1-3) ◽  
pp. 169
Author(s):  
J Langner
Keyword(s):  
Mrna Expression ◽  
Aminopeptidase A
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