Thin Layer Chromatography of Amino Acid

2020 ◽  
pp. 255-257
Author(s):  
Aakanchha Jain ◽  
Richa Jain ◽  
Sourabh Jain
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

scholarly journals Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Beata Polak ◽  
Adam Traczuk ◽  
Sylwia Misztal
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Amino Acid Derivatives ◽  
Solute Retention ◽  
Micellar Systems ◽  
Layer Chromatography

AbstractThe problems with separation of amino acid mixtures in reversed-phase mode are the result of their hydrophilic nature. The derivatisation of the amino group of mentioned above solutes leads to their solution. For this purpose, 9-fluorenylmethoxycarbonyl chloroformate (f-moc-Cl) as the derivatisation reagent is often used. In our study, the separation of some f-moc- amino acid derivatives (alanine, phenylalanine, leucine, methionine, proline and tryptophan) with the use of micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) is investigated. The effect of surfactant concentration, its type (anionic, cationic and non-ionic) and mobile phase buffer pH on the discussed above solute migration distances are presented. Our work reveals that the increase of sodium dodecylsulphate concentration in the mobile phase has a different effect on solute retention in HPTLC and PPEC. Moreover, it also affects the order of solutes in both techniques. In PPEC, in contrast to the HPTLC technique, the mobile phase pH affects solute retention. The type of surfactant in the mobile phase also impacts solute retention and migration distances. A mobile phase containing SDS improves system efficiency in both techniques. Herein, such an effect is presented for the first time.

Effects of Echinostoma caproni miracidia dose on the amino acid contents of Biomphalaria glabrata as determined by high-performance thin-layer chromatography

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Mai Nguyen ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
High Dose ◽  
Echinostoma Caproni ◽  
Amino Acid Contents ◽  
Layer Chromatography

AbstractThe effects of 5, 20, and 40 miracidia dose exposures of Echinostoma caproni on the amino acid contents of Biomphalaria glabrata were studied using high performance thin-layer chromatography-densitometry. Amino acids were identified and quantified in whole bodies of exposed snails and in the uninfected matched controls at 2 and 4 weeks post-exposure. Using cellulose layers with the mobile phase 2-butanol-pyridine-glacial acetic acid-deionized water (39:34:10:26) and ninhydrin detection reagent [2% ninhydrin in acetone-n-butanol (1:1)], five amino acids were identified, i.e., leucine/isoleucine, valine, alanine, glycine, and ornithine, by hRF value comparison and color differentiation. Quantitatively, there was a marked elevation in the amounts of four of these five amino acids (isoleucine/leucine, valine, alanine, and ornithine) across dose levels at 4 weeks post-infection (P<0.05). Elevation of the amino acid content in the high dose snail group suggested that some changes occurred in the amino acid metabolism of the snails in that group as a function of miracidia dose.

scholarly journals Amino acid and vitamin determinations by TLC/HPTLC: review of the current state

2012 ◽  
Vol 10 (3) ◽  
pp. 731-750 ◽  
Author(s):  
Ali Mohammad ◽  
Abdul Moheman ◽  
Gaber El-Desoky
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Tabular Form ◽  
Future Prospects ◽  
Pharmaceutical Samples ◽  
Current State ◽  
Layer Chromatography

AbstractSeveral methods to determine amino acids and vitamins in biological and pharmaceutical samples have been reported. Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable. This review covers the 1991–2010 literature on TLC/HPTLC (high performance thin layer chromatography) amino acid and vitamin determinations. It gives an overview of the special features as well as the problems in TLC/HPTLC determinations of amino acids and vitamins. Various chromatographic systems useful in amino acid and vitamin identification, separation and quantitation of are presented in tabular form. Future prospects of TLC/HPTLC for amino acid and vitamin determinations are also discussed.

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