Isolation and Functional Characterization of Side Population Stem Cells

2008 ◽  
pp. 183-193 ◽  
Author(s):  
Jonathan B. Johnnidis ◽  
Fernando D. Camargo
Keyword(s):  
Stem Cells ◽  
Side Population ◽  
Functional Characterization

scholarly journals Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Abdullah Jawad Alshawaf ◽  
Serena Viventi ◽  
Wanzhi Qiu ◽  
Giovanna D’Abaco ◽  
Bryony Nayagam ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Sensory Neurons ◽  
Human Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem ◽  
Peripheral Sensory Neurons ◽  
Peripheral Sensory

scholarly journals Isolation and Functional Characterization of Equine Adipos Tissue Derived Mesenchymal Stem Cells

2016 ◽  
Vol 10 ◽  
pp. 412-416 ◽  
Author(s):  
Emoke Pall ◽  
Sergiu Toma ◽  
Cristian Crecan ◽  
Mihai Cenariu ◽  
Ioan Groza
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Functional Characterization

scholarly journals Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells

2018 ◽  
Vol 10 (6) ◽  
pp. 1895-1907 ◽  
Author(s):  
Thomas F. Allison ◽  
Andrew J.H. Smith ◽  
Konstantinos Anastassiadis ◽  
Jackie Sloane-Stanley ◽  
Veronica Biga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem

scholarly journals Isolation and functional characterization of murine prostate stem cells

2006 ◽  
Vol 104 (1) ◽  
pp. 181-186 ◽  
Author(s):  
D. A. Lawson ◽  
L. Xin ◽  
R. U. Lukacs ◽  
D. Cheng ◽  
O. N. Witte
Keyword(s):  
Stem Cells ◽  
Functional Characterization

VHL-mutant renal cell carcinomas contain cancer cells with mesenchymal phenotypes.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 4568-4568 ◽  
Author(s):  
Craig Gedye ◽  
Danylo Sirskyj ◽  
Nazleen Carol Lobo ◽  
Andrew Evans ◽  
Neil Eric Fleshner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Renal Cell ◽  
Clear Cell ◽  
Ex Vivo ◽  
Functional Characterization ◽  
Mutant Cells

4568 Background: To study “cancer stem cells” it is imperative to account for all stromal cell populations within the tumour. The existence of “cancer stem cells” in clear cell renal cell carcinoma (ccRCC) has not been examined in ex vivo patient samples. Methods: We established a multiplex flow cytometry (FC) antibody panel in ccRCC, which reliably identified stromal lineages including CD45+ immune, CD31+/CD144+ endothelial and fibroblast-marker-positive subpopulations, thus allowing isolation of "lineage-negative" tumor cells. To verify the identity of tumour-derived populations as either cancer cells or normal stromal cells, we took advantage of the fact that mutations in VHL occur early during ccRCC tumorigenesis and are found in two-thirds of patients. Results: We sequenced 18 patient tumor samples, 12 of which had VHL exome mutations. Targeted re-sequencing of FC sorted subpopulations from these patients’ samples revealed that while CD45+ immune cells and CD31+/CD144+ endothelial cells were genetically normal, a population of VHL-mutant fibroblast-marker positive cells was consistently identified in every patient’s tumour. Immunohistochemistry showed that fibroblast marker-positive VHL-mutant cells do not have the large “clear cell” morphology typical of the majority of the cancer cells in these tumours. When purified and cultured, these fibroblast marker-positive VHL-mutant cells proliferate extensively under mesenchymal culture conditions, but displayed different morphologies to lineage-negative VHL-mutant tumor cells. Functional characterization of these FC sorted cell subpopulations is ongoing, including proliferation, migration, invasion, differentiation and treatment resistance. Conclusions: The phenotype and preliminary functional characterization of these VHL-mutant fibroblast-marker positive cells suggests a mesenchymal differentiation program in ccRCC, with implications for the ontogeny, biology and clinical management of VHL-mutant renal cancer.

scholarly journals Isolation and Functional Characterization of α-Smooth Muscle Actin Expressing Cardiomyocytes from Embryonic Stem Cells

2010 ◽  
Vol 25 (6) ◽  
pp. 595-604 ◽  
Author(s):  
Shiva Prasad Potta ◽  
Huamin Liang ◽  
Johannes Winkler ◽  
Michael Xavier Doss ◽  
Shuhua Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Smooth Muscle Actin ◽  
Functional Characterization ◽  
Embryonic Stem ◽  
Muscle Actin

Characterization of the Human Pancreas Side Population as a Potential Reservoir of Adult Stem Cells

Pancreas ◽  
2018 ◽  
Vol 47 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Petra Augstein ◽  
Thomas Loudovaris ◽  
Esther Bandala-Sanchez ◽  
Peter Heinke ◽  
Gaetano Naselli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Side Population ◽  
Human Pancreas ◽  
Potential Reservoir
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