Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes

2009 ◽  
pp. 177-202 ◽  
Author(s):  
Melanie Carless
Keyword(s):  
Comparative Genomic Hybridization ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Metaphase Chromosomes ◽  
Genomic Aberrations

Comparative Genomic Hybridization to Dna Microarrays

1997 ◽  
Vol 3 (S2) ◽  
pp. 205-206
Author(s):  
D. Pinkel ◽  
R. Segraves ◽  
D. Sudar ◽  
L. van Vliet ◽  
S. Clark ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Copy Number ◽  
Dna Microarrays ◽  
Suppressor Gene ◽  
Comparative Genomic ◽  
Cancer Genes ◽  
Genomic Hybridization ◽  
Metaphase Chromosomes ◽  
Number Variation ◽  
Genetic Events

Comparative genomic hybridization (CGH), which involves the simultaneous hybridization of differentially labeled total genomic DNA from test cells and reference normal cells to metaphase chromosomes, has been used extensively to screen tumor genomes for regions of DNA sequence copy number variation. Analysis of these hybridizations requires quantitative analysis of the ratio of intensities of the fluorescent hybridization signals as a function of position along the chromosomes, which basically serve as a convenient genetic map. The ratios need to be measured very accurately since changes of about ± 20% from the average for the genome indicate important genetic events. Widespread use of CGH over the past several years has identified numerous regions of the genome that may contain currently unknown cancer genes. For example, regions of increased copy number may indicate sites of oncogenes, while regions of copy number decrease relative to average for the genome may signify the presence of a tumor suppressor gene.

Detection of chromosomal imbalances in central neurocytomas by using comparative genomic hybridization

2000 ◽  
Vol 93 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Xiao-Lu Yin ◽  
Jesse Chung-Sean Pang ◽  
Angela Bik-Yu Hui ◽  
Ho-Keung Ng
Keyword(s):  
Dna Sequence ◽  
Comparative Genomic Hybridization ◽  
Dna Sequences ◽  
Immunohistochemical Analysis ◽  
Comparative Genomic ◽  
Fluorescence Intensity Ratio ◽  
Genomic Hybridization ◽  
Metaphase Chromosomes ◽  
Content Type ◽  
Fine Print

Object. Central neurocytomas are rare neuronal tumors commonly found in the intraventricular regions. Little is known about the tumorigenesis of these neoplasms. The aim of this study was to provide an overview of genetic imbalances in central neurocytomas.Methods. In this study, comparative genomic hybridization was used to identify DNA sequence copy number changes (losses and gains) in a series of 10 central neurocytomas. Tumor DNA and normal reference DNA were differentially labeled and allowed to cohybridize to normal metaphase chromosomes. After hybridization and fluorescent staining of the bound DNA, regions of gain or of loss of DNA sequences were detected as changes in the tumor/normal fluorescence intensity ratio along the target metaphase chromosomes. A gain of DNA sequence was detected in chromosomes 2p, 10q, and 18q. A protooncogene, Bcl2, which maps to 18q21, was evaluated by immunohistochemical analysis to determine its role in the formation of central neurocytomas.Conclusions. In this study the authors identified recurrent genetic changes on chromosomes 2p, 10q, and 18q in central neurocytomas and highlighted chromosomal regions for additional mapping and cloning of candidate genes that are important in the development of central neurocytomas.

scholarly journals Breast cancer in young women (?35 years): Genomic aberrations detected by comparative genomic hybridization

2003 ◽  
Vol 107 (4) ◽  
pp. 583-592 ◽  
Author(s):  
Susanne Weber-Mangal ◽  
Hans-Peter Sinn ◽  
Susanne Popp ◽  
R�diger Klaes ◽  
Robert Emig ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Comparative Genomic Hybridization ◽  
Young Women ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Genomic Aberrations
Sign in / Sign up

Export Citation Format

Share Document