Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens

2010 ◽  
pp. 345-357 ◽  
Author(s):  
Koichi Tabeta ◽  
Kazuhisa Yamazaki
Keyword(s):  
Immune Responses ◽  
Periodontal Pathogens ◽  
Recombinant Antigens

scholarly journals Recombinant Proteins of Cryptosporidium parvum Induce Proliferation of Mesenteric Lymph Node Cells in Infected Mice

2005 ◽  
Vol 73 (8) ◽  
pp. 5245-5248 ◽  
Author(s):  
Inderpal Singh ◽  
Cynthia Theodos ◽  
Saul Tzipori
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Recombinant Proteins ◽  
Cryptosporidium Parvum ◽  
Mesenteric Lymph Node ◽  
Recombinant Antigens ◽  
Cellular Immune Responses ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Cellular Immune

ABSTRACT Recombinant antigens of Cryptosporidium parvum, Cp900 and Cp40 but not Cp15, stimulated C. parvum-specific proliferative immune responses of mesenteric lymph node cells in C57BL/6J mice infected with different isolates (MD, GCH1, UCP, and IOWA) of C. parvum, indicating that both Cp900 and Cp40 are immunodominant targets of cellular immune responses during C. parvum infection.

Immune responses elicited by Mycoplasma hyopneumoniae recombinant antigens and DNA constructs with potential for use in vaccination against porcine enzootic pneumonia

Vaccine ◽  
2014 ◽  
Vol 32 (44) ◽  
pp. 5832-5838 ◽  
Author(s):  
Veridiana Gomes Virginio ◽  
Taylor Gonchoroski ◽  
Jéssica Andrade Paes ◽  
Desirée Cigaran Schuck ◽  
Arnaldo Zaha ◽  
...  
Keyword(s):  
Immune Responses ◽  
Mycoplasma Hyopneumoniae ◽  
Enzootic Pneumonia ◽  
Recombinant Antigens

scholarly journals Humoral immune responses to periodontal pathogens in the elderly

2015 ◽  
Vol 45 (5) ◽  
pp. 178 ◽  
Author(s):  
Uttom Shet ◽  
Hee-Kyun Oh ◽  
Hyun-Ju Chung ◽  
Young-Joon Kim ◽  
Ok-Su Kim ◽  
...  
Keyword(s):  
Immune Responses ◽  
The Elderly ◽  
Periodontal Pathogens ◽  
Humoral Immune ◽  
Humoral Immune Responses

scholarly journals Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice

2003 ◽  
Vol 36 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Valéria Rêgo Alves Pereira ◽  
Virginia Maria Barros de Lorena ◽  
Mineo Nakazawa ◽  
Ana Paula Galvão da Silva ◽  
Ulisses Montarroyos ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Immune Responses ◽  
Control Group ◽  
Mechanisms Of Resistance ◽  
Recombinant Antigens ◽  
Cellular Immune Responses ◽  
Cellular Immune ◽  
Antibody Levels ◽  
Cruzi Infection

Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [³H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.

scholarly journals Immune Responses to Recombinant Pneumococcal PspA Antigen Delivered by Live Attenuated Salmonella enterica Serovar Typhimurium Vaccine

2002 ◽  
Vol 70 (4) ◽  
pp. 1739-1749 ◽  
Author(s):  
Ho Young Kang ◽  
Jay Srinivasan ◽  
Roy Curtiss
Keyword(s):  
Immune Responses ◽  
Vaccine Strain ◽  
Salmonella Enterica ◽  
Outer Membrane Proteins ◽  
Oral Immunization ◽  
Recombinant Antigen ◽  
Heterologous Antigen ◽  
Recombinant Antigens ◽  
Serovar Typhimurium ◽  
Type Response

ABSTRACT Attenuated Salmonella enterica serovar Typhimurium expressing recombinant antigens from other pathogens elicits primarily a Th1-type dominant immune response to both recombinant and Salmonella antigens. The immunogenicity and appropriate subcellular location of the recombinant antigen in the Salmonella vaccine strain may contribute to augmenting immune responses by facilitating adequate exposure of recombinant antigen to antigen-presenting cells for processing. To allow for secretion from gram-negative bacteria and overexpression of antigen, a DNA fragment encoding a highly antigenic α-helical region of PspA (pneumococcal surface protein A) was subcloned downstream from the β-lactamase signal sequence in the multicopy Asd+ pYA3493 vector to create pYA3494. pYA3493 was derived from a class of Asd+ vectors with reduced expression of Asd to minimize selective disadvantage and enhance immunization of expressed recombinant antigens. The S. enterica serovar Typhimurium vaccine strain was constructed by the introduction of deletion mutations Δcrp-28 and ΔasdA16. Approximately 50% of the recombinant PspA (rPspA) expressed in a Salmonella strain harboring pYA3494 was detected in the combined supernatant and periplasmic fractions of broth-grown recombinant Salmonella. After a single oral immunization in BALB/c mice with 109 CFU of the recombinant Salmonella vaccine strain carrying pYA3494, immunoglobulin G (IgG) antibody responses were stimulated to both the heterologous antigen rPspA and Salmonella lipopolysaccharide (LPS) and outer membrane proteins (OMPs). About half, and even more at later times after immunization, of the antibodies induced to rPspA were IgG1 (indicating a Th2-type response), whereas 60 to 70% of the antibodies to LPS and 80 to 90% of those to OMPs were IgG2a (indicating a Th1-type response). A sublethal infection with Streptococcus pneumoniae WU2 boosted PspA antibody levels and maintained IgG2a/IgG1 ratios similar to those seen before the challenge. Oral immunization with Salmonella-PspA vaccine protected 60% of immunized mice from death after intraperitoneal challenge with 50 times the 50% lethal dose of virulent S. pneumoniae WU2.

Humoral immune responses in chickens and turkeys after infection with Toxoplasma gondii by using recombinant antigens

2014 ◽  
Vol 113 (4) ◽  
pp. 1473-1480 ◽  
Author(s):  
Andrea Hotop ◽  
Susanne Buschtöns ◽  
Berit Bangoura ◽  
Birte Zöller ◽  
Martin Koethe ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immune Responses ◽  
Recombinant Antigens ◽  
Humoral Immune ◽  
Humoral Immune Responses

scholarly journals NOD1 and NOD2 Mediate Sensing of Periodontal Pathogens

2009 ◽  
Vol 89 (2) ◽  
pp. 186-191 ◽  
Author(s):  
T. Okugawa ◽  
T. Kaneko ◽  
A. Yoshimura ◽  
N. Silverman ◽  
Y. Hara
Keyword(s):  
Immune Responses ◽  
Fusobacterium Nucleatum ◽  
Innate Immune ◽  
Periodontal Pocket ◽  
Innate Immune Responses ◽  
Periodontal Pathogens ◽  
Human Embryonic Kidney Cells ◽  
Oral Epithelial Cells ◽  
Oral Epithelial ◽  
Oligomerization Domain

In bacterial infection, Nucleotide-binding Oligomerization Domain (NOD) 1 and NOD2 induce innate immune responses by recognizing fragments of the bacterial component peptidoglycan (PGN). To determine the roles of these receptors in detection of periodontal pathogens, we stimulated human embryonic kidney cells expressing NOD1 or NOD2 with heat-killed Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum or their soluble PGNs (sPGNs). All bacteria and their sPGNs could stimulate activation of NF-κB. However, there were differences in NOD1- and NOD2-stimulatory activities among the species of bacteria. P. gingivalis showed weaker NOD1- and NOD2-stimulatory activities than did other bacteria. These differences in activities were confirmed by production of interleukin-8 from oral epithelial cells stimulated with sPGNs. These findings indicate that both NOD1 and NOD2 might be involved in the recognition of periodontal pathogens, and that the weak NOD-stimulatory property of P. gingivalis might be helpful for survival in the periodontal pocket.

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