Evaluation of the immune response to CRA and FRA recombinant antigens of Trypanosoma cruzi in C57BL/6 mice

Humoral and cellular immune responses were evaluated in 44 C57BL/6 mice immunized with the Trypanosoma cruzi recombinant antigens CRA and FRA. Both antigens induced cutaneous immediate-type hypersensitivity response. The levels of IgG1, IgG2a, IgG2b and IgG3 were high in CRA immunized mice. IgG3 was the predominant isotype. Although no difference in antibody levels was observed in FRA-immunized mice when compared to control mice, both antigens were able to induce lymphoproliferation in immunized mice. Significant differences were observed between incorporation of [³H]- thymidine by spleen cell stimulated in vitro with CRA or FRA and the control group. These results suggest that CRA and FRA could be involved in mechanisms of resistance to Trypanosoma cruzi infection.

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Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection

Parasitology Research ◽

10.1007/s00436-005-1336-4 ◽

2005 ◽

Vol 96 (3) ◽

pp. 154-161 ◽

Cited By ~ 17

Author(s):

Valéria R. A. Pereira ◽

Virginia M. B. Lorena ◽

Mineo Nakazawa ◽

Carlos F. Luna ◽

Edimilson D. Silva ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Immune Responses ◽

Cellular Immune Responses ◽

Cellular Immune ◽

Cruzi Infection

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A MATHEMATICAL MODEL TO ASSESS THE IMMUNE RESPONSE AGAINSTTRYPANOSOMA CRUZIINFECTION

Journal of Biological System ◽

10.1142/s0218339015500084 ◽

2015 ◽

Vol 23 (01) ◽

pp. 131-163 ◽

Cited By ~ 2

Author(s):

HYUN MO YANG

Keyword(s):

Mathematical Model ◽

Trypanosoma Cruzi ◽

Immune Responses ◽

Cellular Response ◽

Humoral Response ◽

Cellular Responses ◽

Cellular Immune Responses ◽

Trivial Equilibrium ◽

Cellular Immune ◽

Cruzi Infection

A mathematical model is developed to assess humoral and cellular immune responses against Trypanosoma cruzi infection. Analysis of the model shows a unique non-trivial equilibrium, which is locally asymptotically stable, except in the case of a strong cellular response. When the proliferation of the activated CD8 T cells is increased, this equilibrium becomes unstable and a limit cycle appears. However, this behavior can be avoided by increasing the action of the humoral response. Therefore, unbalanced humoral and cellular responses can be responsible for long asymptomatic period, and the control of Trypanosoma cruzi infection is a consequence of well coordinated action of both humoral and cellular responses.

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In vitro cellular immune responses to complex and newly defined recombinant antigens of Mycobacterium tuberculosis

Clinical & Experimental Immunology ◽

10.1111/j.1365-2249.2004.02609.x ◽

2004 ◽

Vol 138 (1) ◽

pp. 139-144 ◽

Cited By ~ 33

Author(s):

R. AL-ATTIYAH ◽

A. S. MUSTAFA ◽

A. T. ABAL ◽

A. S. M. EL-SHAMY ◽

W. DALEMANS ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Immune Responses ◽

Recombinant Antigens ◽

Cellular Immune Responses ◽

Cellular Immune

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Salmonella Adhesion, Invasion and Cellular Immune Responses Are Differentially Affected by Iron Concentrations in a Combined In Vitro Gut Fermentation-Cell Model

PLoS ONE ◽

10.1371/journal.pone.0093549 ◽

2014 ◽

Vol 9 (3) ◽

pp. e93549 ◽

Cited By ~ 29

Author(s):

Alexandra Dostal ◽

Mélanie Gagnon ◽

Christophe Chassard ◽

Michael Bruce Zimmermann ◽

Liam O'Mahony ◽

...

Keyword(s):

Immune Responses ◽

Cell Model ◽

Cellular Immune Responses ◽

Cellular Immune ◽

Gut Fermentation ◽

Iron Concentrations

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A Combined Adjuvant TF–Al Consisting of TFPR1 and Aluminum Hydroxide Augments Strong Humoral and Cellular Immune Responses in Both C57BL/6 and BALB/c Mice

Vaccines ◽

10.3390/vaccines9121408 ◽

2021 ◽

Vol 9 (12) ◽

pp. 1408

Author(s):

Qiao Li ◽

Zhihua Liu ◽

Yi Liu ◽

Chen Liang ◽

Jiayi Shu ◽

...

Keyword(s):

Immune Responses ◽

Vaccine Development ◽

Inbred Mouse ◽

Effective Vaccine ◽

Mouse Strains ◽

Cellular Immune Responses ◽

Recombinant Hbsag ◽

Cellular Immune

TFPR1 is a novel adjuvant for protein and peptide antigens, which has been demonstrated in BALB/c mice in our previous studies; however, its adjuvanticity in mice with different genetic backgrounds remains unknown, and its adjuvanticity needs to be improved to fit the requirements for various vaccines. In this study, we first compared the adjuvanticity of TFPR1 in two commonly used inbred mouse strains, BALB/c and C57BL/6 mice, in vitro and in vivo, and demonstrated that TFPR1 activated TLR2 to exert its immune activity in vivo. Next, to prove the feasibility of TFPR1 acting as a major component of combined adjuvants, we prepared a combined adjuvant, TF–Al, by formulating TFPR1 and alum at a certain ratio and compared its adjuvanticity with that of TFPR1 and alum alone using OVA and recombinant HBsAg as model antigens in both BALB/c and C57BL/6 mice. Results showed that TFPR1 acts as an effective vaccine adjuvant in both BALB/c mice and C57BL/6 mice, and further demonstrated the role of TLR2 in the adjuvanticity of TFPR1 in vivo. In addition, we obtained a novel combined adjuvant, TF–Al, based on TFPR1, which can augment antibody and cellular immune responses in mice with different genetic backgrounds, suggesting its promise for vaccine development in the future.

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Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection

Journal of Tropical Medicine ◽

10.1155/2015/523560 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Reza Taherkhani ◽

Fatemeh Farshadpour ◽

Manoochehr Makvandi ◽

Hamid Rajabi Memari ◽

Ali Reza Samarbafzadeh ◽

...

Keyword(s):

Cell Proliferation ◽

Immune Responses ◽

Mononuclear Cells ◽

Hepatitis E ◽

Control Group ◽

Peripheral Blood Mononuclear ◽

Orf2 Protein ◽

Cellular Immune ◽

Iranian Patients

Background.The aim of this study was to evaluatehepatitis E virus(HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine.Methods.A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups.Results.The truncated ORF2 protein was able to induce IFN-γELISPOT and cell proliferation responses and to produce significant amounts of IFN-γand IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokinesin vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines.Conclusion.The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this proteinin vivo.

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Cellular immune responses to autologous chronic myelogenous leukaemia cells in vitro

Cancer Immunology Immunotherapy ◽

10.1007/s002620050270 ◽

1996 ◽

Vol 42 (3) ◽

pp. 193-199 ◽

Cited By ~ 24

Author(s):

Graham Pawelec ◽

Arnika Rehbein ◽

Elke Schlotz ◽

Paul da Silva

Keyword(s):

Immune Responses ◽

Chronic Myelogenous Leukaemia ◽

Myelogenous Leukaemia ◽

Cellular Immune Responses ◽

Cellular Immune

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Humoral and Cellular Immune Responses to Trypanosoma cruzi-Derived Paraflagellar Rod Proteins in Patients with Chagas' Disease

Infection and Immunity ◽

10.1128/iai.71.6.3165-3171.2003 ◽

2003 ◽

Vol 71 (6) ◽

pp. 3165-3171 ◽

Cited By ~ 34

Author(s):

Vladimir Michailowsky ◽

Keith Luhrs ◽

Manoel Otávio C. Rocha ◽

David Fouts ◽

Ricardo T. Gazzinelli ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Immune Responses ◽

Chagas Disease ◽

Mononuclear Cells ◽

Clinical Symptoms ◽

Cellular Responses ◽

Peripheral Blood Mononuclear ◽

Cellular Immune Responses ◽

Ifn Γ ◽

Cellular Immune

ABSTRACT Sera and peripheral blood mononuclear cells (PBMC) from patients displaying different clinical symptoms as well as from normal uninfected individuals (NI) were used to evaluate the humoral and cellular responses of Chagas' disease patients to Trypanosoma cruzi-derived paraflagellar rod proteins (PFR). Our results show that sera from both asymptomatic Chagas' disease patients (ACP) and cardiac Chagas' disease patients (CCP) have higher levels of antibodies to PFR than sera from NI. Immunoglobulin G1 (IgG1) and IgG3 were the main Ig isotypes that recognized PFR. We also tested three recombinant forms of PFR, named rPAR-1, rPAR-2, and rPAR-3, by Western blot analysis. Sera from seven out of eight patients with Chagas' disease recognized one of the three rPAR forms. Sera from 75, 50, and 37.5% of Chagas' disease patients tested recognized rPAR-3, rPAR-2, and rPAR-1, respectively. PFR induced proliferation of 100 and 70% of PBMC from ACP and CCP, respectively. Further, stimulation of cells from Chagas' disease patients with PFR enhanced the frequencies of both small and large CD4+ CD25+ and CD4+ CD69+ lymphocytes, as well as that of small CD8+ CD25+ lymphocytes. Finally, we evaluated the ability of PFR to elicit the production of gamma interferon (IFN-γ) by PBMC from patients with Chagas' disease. Fifty percent of the PBMC from ACP as well as CCP produced IFN-γ upon stimulation with PFR. PFR enhanced the percentages of IFN-γ-producing cells in both CD3+ and CD3− populations. Within the T-cell population, large CD4+ T lymphocytes were the main source of IFN-γ.

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