Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins

2012 ◽  
pp. 301-317 ◽  
Author(s):  
Florence Baudin ◽  
Christoph W. Müller
Keyword(s):  
Bacterial Expression ◽  
Stat Proteins

Targeting the JAK/STAT Signaling Pathway for Breast Cancer.

2020 ◽  
Vol 28 ◽  
Author(s):  
Fei Shao ◽  
Xiaonan Pang ◽  
Gyeong Hun Baeg
Keyword(s):  
Breast Cancer ◽  
Signal Transduction ◽  
Signaling Pathway ◽  
Signal Transduction Pathway ◽  
Breast Cancer Treatment ◽  
Review Article ◽  
Transduction Pathway ◽  
Inhibitory Effects ◽  
Stat Proteins ◽  
Stat Signaling

Abstract:: Breast cancer is the most common malignant tumor in women worldwide. Traditional ways of treatment, includ-ing radiotherapy and endocrine therapy, for breast cancer have inevitable side effects. In recent decades, targeted therapies for breast cancer have rapidly advanced and shown a promising future. The JAK/STAT signaling pathway has been shown to play important roles in tumorigenesis, maintenance and metastasis of breast cancer. Hence, many small molecule inhibi-tors of JAK and STAT proteins have been developed. These inhibitors exhibit potent inhibitory effects on breast cancer in both cellular and animal models, and even some of them have already been in clinical trials. This review article discussed the JAK/STAT signal transduction pathway in the pathogenesis of breast cancer, and the potential for the application of JAK/STAT inhibitors in breast cancer treatment.

Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli

1999 ◽  
Vol 64 (8) ◽  
pp. 1348-1356 ◽  
Author(s):  
Michaela Rumlová-Kliková ◽  
Iva Pichová ◽  
Eric Hunter ◽  
Tomáš Ruml
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Amorphous Material ◽  
Bacterial Expression ◽  
Biologically Active ◽  
Capsid Assembly ◽  
Cultivation Medium ◽  
Viral Particles ◽  
Induction Temperature

It has been generally accepted that inclusion bodies (IBs) formed in Escherichia coli consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression.

Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 from Allium cepa

2017 ◽  
Vol 255 ◽  
pp. 9-15 ◽  
Author(s):  
Ruxin Cai ◽  
Caihong Chen ◽  
Yan Li ◽  
Kaiyan Sun ◽  
Fangfang Zhou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Bacterial Expression

Comparative Analysis of Bacterial Expression Systems for Keratinase Production

2014 ◽  
Vol 173 (5) ◽  
pp. 1222-1235 ◽  
Author(s):  
Baihong Liu ◽  
Juan Zhang ◽  
Lei Gu ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems
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