Visualization of Fas-Mediated Death-Inducing Signaling Complex Formation by Immunoprecipitation

2013 ◽  
pp. 43-49 ◽  
Author(s):  
Nelia Cordeiro ◽  
Nicolas Bidère
Keyword(s):  
Complex Formation ◽  
Signaling Complex

scholarly journals Synergistic Interaction of MEK Kinase 2, c-Jun N-Terminal Kinase (JNK) Kinase 2, and JNK1 Results in Efficient and Specific JNK1 Activation

2000 ◽  
Vol 20 (7) ◽  
pp. 2334-2342 ◽  
Author(s):  
Jinke Cheng ◽  
Jianhua Yang ◽  
Ying Xia ◽  
Michael Karin ◽  
Bing Su
Keyword(s):  
Complex Formation ◽  
Molecular Basis ◽  
Synergistic Interaction ◽  
Specific Flow ◽  
Mitogen Activated Protein Kinases ◽  
Mapk Kinase ◽  
Signaling Complex ◽  
Mapk Cascades ◽  
Mitogen Activated Protein ◽  
Flow Of Information

ABSTRACT Mitogen-activated protein kinases (MAPKs) are activated through cascades or modules consisting of a MAPK, a MAPK kinase (MAPKK), and a MAPKK kinase (MAPKKK). Investigating the molecular basis of activation of the c-Jun N-terminal kinase (JNK) subgroup of MAPK by the MAPKKK MEKK2, we found that strong and specific JNK1 activation by MEKK2 was mediated by the MAPKK JNK kinase 2 (JNKK2) rather than by JNKK1 through formation of a tripartite complex consisting of MEKK2, JNKK2, and JNK1. No scaffold protein was required for the MEKK2-JNKK2-JNK1 tripartite-complex formation. Expression of JNK1, JNKK2, and MEKK2 significantly augmented the coprecipitation of, respectively, MEKK2-JNKK2, MEKK2-JNK1, and JNKK2-JNK1, indicating that the interaction of MEKK2, JNKK2, and JNK1 is synergistic. Finally, the JNK1 was activated more efficiently in the MEKK2-JNKK2-JNK1 complex than was the JNK1 excluded from the complex. Thus, formation of a signaling complex through synergistic interaction of a MAPKKK, a MAPKK, and a MAPK molecule like MEKK2-JNKK2-JNK1 is likely to be responsible for the efficient, specific flow of information via MAPK cascades.

scholarly journals Modeling TCR Signaling Complex Formation in Positive Selection

2003 ◽  
Vol 171 (6) ◽  
pp. 2825-2831 ◽  
Author(s):  
Katherine J. Hare ◽  
Judit Pongracz ◽  
Eric J. Jenkinson ◽  
Graham Anderson
Keyword(s):  
Complex Formation ◽  
Positive Selection ◽  
Tcr Signaling ◽  
Signaling Complex

scholarly journals CD95 death-inducing signaling complex formation and internalization occur in lipid rafts of type I and type II cells

2004 ◽  
Vol 34 (7) ◽  
pp. 1930-1940 ◽  
Author(s):  
Adriana Eramo ◽  
Massimo Sargiacomo ◽  
Lucia Ricci-Vitiani ◽  
Matilde Todaro ◽  
Giorgio Stassi ◽  
...  
Keyword(s):  
Complex Formation ◽  
Lipid Rafts ◽  
Type I ◽  
Type Ii Cells ◽  
Type Ii ◽  
Signaling Complex

scholarly journals Inhibition of Glucose Metabolism Sensitizes Tumor Cells to Death Receptor-triggered Apoptosis through Enhancement of Death-inducing Signaling Complex Formation and Apical Procaspase-8 Processing

2003 ◽  
Vol 278 (15) ◽  
pp. 12759-12768 ◽  
Author(s):  
Cristina Muñoz-Pinedo ◽  
Carmen Ruiz-Ruiz ◽  
Carmen Ruiz de Almodóvar ◽  
Carmen Palacios ◽  
Abelardo López-Rivas
Keyword(s):  
Glucose Metabolism ◽  
Complex Formation ◽  
Tumor Cells ◽  
Death Receptor ◽  
Signaling Complex

Signaling complex formation of CD44 with src-related kinases

1999 ◽  
Vol 68 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Zoltan Rozsnyay
Keyword(s):  
Complex Formation ◽  
Signaling Complex

scholarly journals Quantitative Fluorescence Microscopy Reveals Fibroblast Growth Factor Receptor 5 Signaling Complex Formation

2016 ◽  
Vol 110 (3) ◽  
pp. 373a
Author(s):  
Pamuditha N. Silva ◽  
Romario Regeenes ◽  
Nicholas K. Wang ◽  
Dawn M. Kilkenny ◽  
Jonathan V. Rocheleau
Keyword(s):  
Growth Factor ◽  
Complex Formation ◽  
Fluorescence Microscopy ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fibroblast Growth ◽  
Signaling Complex ◽  
Quantitative Fluorescence
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