Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement

2013 ◽  
pp. 305-317 ◽  
Author(s):  
Matthieu Broussas ◽  
Lucile Broyer ◽  
Liliane Goetsch
Keyword(s):  
Lactate Dehydrogenase ◽  
Cell Cytotoxicity ◽  
Dependent Cell

Antibody-Dependent Cell Cytotoxicity (ADCC).

1976 ◽  
Vol 5 (3) ◽  
pp. 233-242 ◽  
Author(s):  
G. CORDIER ◽  
C. SAMARUT ◽  
J. BROCHIER ◽  
J. P. REVILLARD
Keyword(s):  
Cell Cytotoxicity ◽  
Dependent Cell

scholarly journals In VitroAssessment of the Immunological Significance of a Human Monoclonal Antibody Directed to the Influenza A Virus Nucleoprotein

2013 ◽  
Vol 20 (8) ◽  
pp. 1333-1337 ◽  
Author(s):  
Rogier Bodewes ◽  
Martina M. Geelhoed-Mieras ◽  
Jens Wrammert ◽  
Rafi Ahmed ◽  
Patrick C. Wilson ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Influenza A ◽  
Viral Antigen ◽  
Human Monoclonal Antibody ◽  
Cell Cytotoxicity ◽  
Influenza A Viruses ◽  
Dependent Cell ◽  
Infected Cells

ABSTRACTInfluenza A viruses cause annual epidemics and occasionally pandemics. Antibodies directed to the conserved viral nucleoprotein (NP) may play a role in immunity against various influenza A virus subtypes. Here, we assessed the immunological significance of a human monoclonal antibody directed to NPin vitro. This antibody bound to virus-infected cells but did not display virus-neutralizing activity, complement-dependent cell cytotoxicity, or opsonization of viral antigen for improved antigen presentation to CD8+T cells by dendritic cells.

Characterization of Bovine Leukocytes Involved in Antibody-Dependent Cell Cytotoxicity

1979 ◽  
Vol 60 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Amarjit S. Grewal ◽  
Barry T. Rouse
Keyword(s):  
Cell Cytotoxicity ◽  
Dependent Cell

scholarly journals Heterogeneous neutralizing antibody and antibody-dependent cell cytotoxicity responses in HIV-1 elite controllers

AIDS ◽  
2009 ◽  
Vol 23 (8) ◽  
pp. 897-906 ◽  
Author(s):  
Olivier Lambotte ◽  
Guido Ferrari ◽  
Christiane Moog ◽  
Nicole L Yates ◽  
Hua-Xin Liao ◽  
...  
Keyword(s):  
Neutralizing Antibody ◽  
Cell Cytotoxicity ◽  
Elite Controllers ◽  
Dependent Cell ◽  
Hiv 1

Antibody-dependent cell cytotoxicity in chemical-induced rat pancreas and colon cancer

1981 ◽  
Vol 25 (2) ◽  
pp. 372-380 ◽  
Author(s):  
R.H. Stevens ◽  
D.A. Cole ◽  
E.S. Meek
Keyword(s):  
Colon Cancer ◽  
Cell Cytotoxicity ◽  
Rat Pancreas ◽  
Dependent Cell

Tumor cell lysis and synergistically enhanced antibody-dependent cell-mediated cytotoxicity by NKG2D engagement with a bispecific immunoligand targeting the HER2 antigen

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Christian Kellner ◽  
Sebastian Lutz ◽  
Hans-Heinrich Oberg ◽  
Daniela Wesch ◽  
Anna Otte ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Nk Cell ◽  
Growth Factor Receptor ◽  
Cell Cytotoxicity ◽  
Dependent Cell ◽  
Epidermal Growth ◽  
Nk Cell Cytotoxicity ◽  
Tumor Cell Lysis

Abstract Natural killer group 2 member D (NKG2D) plays an important role in the regulation of natural killer (NK) cell cytotoxicity in cancer immune surveillance. With the aim of redirecting NK cell cytotoxicity against tumors, the NKG2D ligand UL-16 binding protein 2 (ULBP2) was fused to a single-chain fragment variable (scFv) targeting the human epidermal growth factor receptor 2 (HER2). The resulting bispecific immunoligand ULBP2:HER2-scFv triggered NK cell-mediated killing of HER2-positive breast cancer cells in an antigen-dependent manner and required concomitant interaction with NKG2D and HER2 as revealed in antigen blocking experiments. The immunoligand induced tumor cell lysis dose-dependently and was effective at nanomolar concentrations. Of note, ULBP2:HER2-scFv sensitized tumor cells for antibody-dependent cell-mediated cytotoxicity (ADCC). In particular, the immunoligand enhanced ADCC by cetuximab, a therapeutic antibody targeting the epidermal growth factor receptor (EGFR) synergistically. No significant improvements were obtained by combining cetuximab and anti-HER2 antibody trastuzumab. In conclusion, dual-dual targeting by combining IgG1 antibodies with antibody constructs targeting another tumor associated antigen and engaging NKG2D as a second NK cell trigger molecule may be promising. Thus, the immunoligand ULBP2:HER2-scFv may represent an attractive biological molecule to promote NK cell cytotoxicity against tumors and to boost ADCC.

scholarly journals Evaluation of antibody-dependent cell cytotoxicity (ADCC) in lung cancer cell lines treated with combined anti-EGFR and anti-PD-L1 therapy

2019 ◽  
Vol 30 ◽  
pp. v772-v773
Author(s):  
F. Sparano ◽  
G. Barra ◽  
C.M. Della Corte ◽  
R. Di Liello ◽  
M. Fasano ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cell Cytotoxicity ◽  
Lung Cancer Cell ◽  
Dependent Cell
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