High-Throughput Label-Free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor

2013 ◽  
pp. 181-200 ◽  
Author(s):  
Sunmin Ahn ◽  
David S. Freedman ◽  
Xirui Zhang ◽  
M. Selim Ünlü
Keyword(s):  
High Throughput ◽  
Dna Hybridization ◽  
Label Free ◽  
Label Free Detection ◽  
Mismatch Discrimination ◽  
Imaging Sensor

scholarly journals Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET

2017 ◽  
Vol 22 (10) ◽  
pp. 1203-1210 ◽  
Author(s):  
Katrin Beeman ◽  
Jens Baumgärtner ◽  
Manuel Laubenheimer ◽  
Karlheinz Hergesell ◽  
Martin Hoffmann ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Maldi Ms ◽  
Kinase Assay ◽  
Label Free ◽  
Screening Process ◽  
Label Free Detection ◽  
Ic50 Values

Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated “in-line reader” for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components. Here we adapt our c-MET kinase assay to optimize for MALDI-MS compatibility and test its feasibility for compound screening. The pros and cons of the Echo (Labcyte) as a transfer system for in situ MALDI-MS sample preparation are discussed. We demonstrate that this method generates robust data in a 1536-grid format. We use the MALDI-MS to directly measure the ratio of c-MET substrate and phosphorylated product to acquire IC50 curves and demonstrate that the pharmacology is unaffected. The resulting IC50 values correlate well between the common label-based capillary electrophoresis and the label-free MALDI-MS detection method. We predict that label-free MALDI-MS-based high-throughput screening will become increasingly important and more widely used for drug discovery.

scholarly journals MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

2017 ◽  
Vol 22 (10) ◽  
pp. 1246-1252 ◽  
Author(s):  
Kishore Kumar Jagadeesan ◽  
Simon Ekström
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Web Application ◽  
Low Cost ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Label Free ◽  
Label Free Detection ◽  
R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

scholarly journals Label-free detection of morpholino-DNA hybridization using a silicon photonics suspended slab micro-ring resonator

2021 ◽  
pp. 1-1
Author(s):  
Soha Yousuf ◽  
Jongmin Kim ◽  
Ajymurat Orozaliev ◽  
Marcus Dahlem ◽  
Yong-Ak Song ◽  
...  
Keyword(s):  
Silicon Photonics ◽  
Dna Hybridization ◽  
Ring Resonator ◽  
Label Free ◽  
Label Free Detection

Label-Free Detection of DNA Hybridization Utilizing Dual S-Tapered Thin-Core Fiber Interferometer

2019 ◽  
Vol 37 (11) ◽  
pp. 2762-2767 ◽  
Author(s):  
Xu Zhang ◽  
Bo Liu ◽  
Hao Zhang ◽  
Xu Zhang ◽  
Binbin Song ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Label Free Detection ◽  
Core Fiber

scholarly journals Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

2006 ◽  
Vol 103 (4) ◽  
pp. 921-926 ◽  
Author(s):  
A. Star ◽  
E. Tu ◽  
J. Niemann ◽  
J.-C. P. Gabriel ◽  
C. S. Joiner ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Dna Hybridization ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free ◽  
Label Free Detection ◽  
Carbon Nanotube Network

Ultrasensitive label-free detection of DNA hybridization by sapphire-based graphene field-effect transistor biosensor

2018 ◽  
Vol 427 ◽  
pp. 1114-1119 ◽  
Author(s):  
Shicai Xu ◽  
Shouzhen Jiang ◽  
Chao Zhang ◽  
Weiwei Yue ◽  
Yan Zou ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Label Free ◽  
Graphene Field Effect Transistor ◽  
Label Free Detection ◽  
Effect Transistor

High-Throughput and Label-Free Detection of Motile Parasites in Bodily Fluids Using Lensless Time-Resolved Speckle Imaging

2019 ◽  
Author(s):  
Yibo Zhang ◽  
Hatice Ceylan Koydemir ◽  
Michelle M. Shimogawa ◽  
Sener Yalcin ◽  
Alexander Guziak ◽  
...  
Keyword(s):  
High Throughput ◽  
Label Free ◽  
Speckle Imaging ◽  
Time Resolved ◽  
Bodily Fluids ◽  
Label Free Detection

Label free detection of DNA hybridization by refractive index tapered fiber biosensor

2010 ◽  
Author(s):  
M. I. Zibaii ◽  
H. Latifi ◽  
E. Ghanati ◽  
M. Gholami ◽  
S. M. Hosseini
Keyword(s):  
Refractive Index ◽  
Dna Hybridization ◽  
Label Free ◽  
Tapered Fiber ◽  
Label Free Detection

Label-free detection of DNA hybridization based on poly(indole-5-carboxylic acid) conducting polymer

2008 ◽  
Vol 622 (1-2) ◽  
pp. 104-110 ◽  
Author(s):  
Xuemei Li ◽  
Jianping Xia ◽  
Shusheng Zhang
Keyword(s):  
Carboxylic Acid ◽  
Conducting Polymer ◽  
Dna Hybridization ◽  
Label Free ◽  
Label Free Detection
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