MNAzyme qPCR: A Superior Tool for Multiplex qPCR

2013 ◽  
pp. 31-49 ◽  
Author(s):  
Elisa Mokany ◽  
Alison V. Todd
Keyword(s):  
Multiplex Qpcr

Optimizing, validating, and field testing a multiplex qPCR for the detection of amphibian pathogens

2018 ◽  
Vol 129 (1) ◽  
pp. 1-13 ◽  
Author(s):  
I Standish ◽  
E Leis ◽  
N Schmitz ◽  
J Credico ◽  
S Erickson ◽  
...  
Keyword(s):  
Field Testing ◽  
Multiplex Qpcr ◽  
Amphibian Pathogens

scholarly journals Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with built-in screening functionality for del-HV69/70- and N501Y variants such as B.1.1.7

2021 ◽  
pp. 104894
Author(s):  
Dominik Nörz ◽  
Moritz Grunwald ◽  
Flaminia Olearo ◽  
Nicole Fischer ◽  
Martin Aepfelbacher ◽  
...  
Keyword(s):  
High Throughput ◽  
Qpcr Assay ◽  
Multiplex Qpcr

scholarly journals Multiplex Real-Time Polymerase Chain Reaction for Simultaneous Quantification ofSalmonellaspp.,Escherichia coli, andStaphylococcus aureusin Different Food Matrices: Advantages and Disadvantages

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Amanda Teixeira Sampaio Lopes ◽  
George Rêgo Albuquerque ◽  
Bianca Mendes Maciel
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Limit Of Detection ◽  
Microbiological Quality ◽  
Rapid Screening ◽  
Multiplex Qpcr ◽  
Advantages And Disadvantages ◽  
Simultaneous Quantification ◽  
Polymerase Chain ◽  
Food Matrices

Quantitative real-time polymerase chain reactions (qPCRs) of the most prevalent bacteria causing foodborne diseases worldwide, such asSalmonellaspp.,Escherichia coli, andStaphylococcus aureus,can be an important tool for quantitative microbial risk assessment, which requires numerical data to determine the level of contamination at a specific stage of food production. However, most of qPCR assays described in the literature for these pathogens are qualitative; their objective is pathogen detection and not pathogen quantification. Thus, the aim of our work was to develop a qPCR for the simultaneous quantification ofSalmonellaspp.,E. coli, andS. aureusand to propose its use in the analysis of foods, as a tool for microbiological quality monitoring. For this, a multiplex qPCR was standardized for the simultaneous quantification of specific fragments of target genes (ssf,phoA, andnuc) corresponding to each one of the mentioned bacteria. The limit of detection of the technique was 13, 10, and 12 gene copies forssf,phoA, andnuc, respectively; standard curves showed R2> 0.99, with efficiencies ranging from 99 to 110%, and inter- and intraexperiment reproducibility presented a low coefficient of variation in all trials. This methodology was applied in different food matrices (milk, ground beef, and oyster meat), and the results were compared with official microbiological culture methodology and with ready-to-use test. Advantages and disadvantages of each methodology used in this study are pointed out. We suggest that this multiplex qPCR can be used as a rapid screening technique for the analysis of food microbiological quality.

Multiplex qPCR assay for simultaneous quantification of CYP51‐S524T and SdhC‐H152R substitutions in European populations of Zymoseptoria tritici

2020 ◽  
Vol 69 (9) ◽  
pp. 1666-1677
Author(s):  
Pierre Hellin ◽  
Maxime Duvivier ◽  
Aurélie Clinckemaillie ◽  
Charlotte Bataille ◽  
Anne Legrève ◽  
...  
Keyword(s):  
Qpcr Assay ◽  
Zymoseptoria Tritici ◽  
Multiplex Qpcr ◽  
Simultaneous Quantification ◽  
European Populations

Multiplex qPCR assay for assessment of reference gene expression stability in rat tissues/samples

2020 ◽  
Vol 53 ◽  
pp. 101611 ◽  
Author(s):  
Alexander P. Schwarz ◽  
Daria A. Malygina ◽  
Anna A. Kovalenko ◽  
Alexander N. Trofimov ◽  
Aleksey V. Zaitsev
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Qpcr Assay ◽  
Rat Tissues ◽  
Expression Stability ◽  
Gene Expression Stability ◽  
Multiplex Qpcr ◽  
Reference Gene Expression

scholarly journals Field application of a novel multiplex qPCR assay reveals the occurrence of the zoonotic hookworm Ancylostoma braziliense in Nigerian dogs

Acta Tropica ◽  
2021 ◽  
Vol 213 ◽  
pp. 105758
Author(s):  
Luca Massetti ◽  
Joshua Kamani ◽  
Anke Wiethoelter ◽  
Phillip McDonagh ◽  
Vito Colella ◽  
...  
Keyword(s):  
Field Application ◽  
Qpcr Assay ◽  
Multiplex Qpcr

scholarly journals Whole Genome Sequencing and Multiplex qPCR Methods to Identify Campylobacter jejuni Encoding cst-II or cst-III Sialyltransferase

2018 ◽  
Vol 9 ◽  
Author(s):  
Jason M. Neal-McKinney ◽  
Kun C. Liu ◽  
Karen C. Jinneman ◽  
Wen-Hsin Wu ◽  
Daniel H. Rice
Keyword(s):  
Whole Genome Sequencing ◽  
Campylobacter Jejuni ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Multiplex Qpcr
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