Cell Culturing: A Beginner’s Guide to Understanding the Basics of Cell Culturing

2008 ◽  
pp. 31-37
Author(s):  
Khurshid Alam ◽  
Edwin T. Anthony ◽  
P. N. Vaiude ◽  
Faruquz Zaman ◽  
Harshad A. Navsaria
Keyword(s):  
Cell Culturing

Modelling neuroendocrine tumours using 3 dimensional cell culturing

2020 ◽  
Author(s):  
Lilla Krokker ◽  
Borbála Szabó ◽  
Kinga Németh ◽  
Balázs Sarkadi ◽  
Katalin Mészáros ◽  
...  
Keyword(s):  
Neuroendocrine Tumours ◽  
3 Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Molecularly imprinted polymers for the detection of viruses: challenges and opportunities

The Analyst ◽  
2021 ◽  
Author(s):  
Ghazaleh Jamalipour Soufi ◽  
Siavash Iravani ◽  
Rajender S Varma
Keyword(s):  
Drug Delivery ◽  
Drug Discovery ◽  
Structure Determination ◽  
Molecularly Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Cell Culturing ◽  
Challenges And Opportunities

Molecularly imprinted polymers (MIPs) have numerous applications in sensing field, the detection/recognition of virus, the structure determination of proteins, drug delivery, artificial/biomimetic antibodies, drug discovery, and cell culturing. There are...

Cell culturing in a three-dimensional matrix affects the localization and properties of plasma membrane cholesterol

2009 ◽  
Vol 33 (10) ◽  
pp. 1079-1086 ◽  
Author(s):  
Nadezhda Stefanova ◽  
Galya Staneva ◽  
Diana Petkova ◽  
Teodora Lupanova ◽  
Roumen Pankov ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Three Dimensional ◽  
Membrane Cholesterol ◽  
Dimensional Matrix ◽  
Cell Culturing ◽  
Plasma Membrane Cholesterol

Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing

2012 ◽  
Vol 22 (11) ◽  
pp. 115016 ◽  
Author(s):  
Elli Käpylä ◽  
Dogu Baran Aydogan ◽  
Sanni Virjula ◽  
Sari Vanhatupa ◽  
Susanna Miettinen ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Direct Laser Writing ◽  
Geometrical Analysis ◽  
Laser Writing ◽  
Cell Culturing ◽  
Pore Architecture ◽  
Direct Laser ◽  
Dimensional Cell

scholarly journals Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing

2009 ◽  
Vol 214 (5) ◽  
pp. 759-767 ◽  
Author(s):  
Florian Haasters ◽  
Wolf Christian Prall ◽  
David Anz ◽  
Carole Bourquin ◽  
Christoph Pautke ◽  
...  
Keyword(s):  
Quality Control ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Cell Culturing

Degradation of Ti–6Al–4V alloy under cyclic loading in a simulated body environment with cell culturing

2016 ◽  
Vol 56 ◽  
pp. 6-13 ◽  
Author(s):  
Kotaro Doi ◽  
Sayaka Miyabe ◽  
Hiroaki Tsuchiya ◽  
Shinji Fujimoto
Keyword(s):  
Cyclic Loading ◽  
Cell Culturing

scholarly journals Microfluidic Bioreactors for Cell Culturing: A Review

2011 ◽  
Vol 3 (2) ◽  
pp. 137-160 ◽  
Author(s):  
Godfrey Pasirayi ◽  
Vincent Auger ◽  
Simon M. Scott ◽  
Pattanathu K.S.M. Rahman ◽  
Meez Islam ◽  
...  
Keyword(s):  
Cell Culturing

scholarly journals 3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches

2019 ◽  
Vol 6 (2) ◽  
pp. 30 ◽  
Author(s):  
Chiara Campiglio ◽  
Francesca Ceriani ◽  
Lorenza Draghi
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
Closed Loop ◽  
Fiber Diameter ◽  
Cross Linking ◽  
Scaffold Fabrication ◽  
Cell Culturing ◽  
Viable Cells ◽  
Flow Circuit

To fully exploit the potential of hydrogel micro-fibers in the design of regenerative medicinal materials, we designed a simple, easy to replicate system for cell embedding in degradable fibrous scaffolds, and validated its effectiveness using alginate-based materials. For scaffold fabrication, cells are suspended in a hydrogel-precursor and injected in a closed-loop circuit, where a pump circulates the ionic cross-linking solution. The flow of the cross-linking solution stretches and solidifies a continuous micro-scaled, cell-loaded hydrogel fiber that whips, bends, and spontaneously assembles in a self-standing, spaghetti-like patch. After investigation and tuning of process- and solution-related parameters, homogeneous microfibers with controlled diameters and consistent scaffolds were obtained from different alginate concentrations and blends with biologically favorable macromolecules (i.e., gelatin or hyaluronic acid). Despite its simplicity, this coaxial-flow encapsulation system allows for the rapid and effortless fabrication of thick, well-defined scaffolds, with viable cells being homogeneously distributed within the fibers. The reduced fiber diameter and the inherent macro-porous structure that is created from the random winding of fibers can sustain mass transport, and support encapsulated cell survival. As different materials and formulations can be processed to easily create homogeneously cell-populated structures, this system appears as a valuable platform, not only for regenerative medicine, but also, more in general, for 3D cell culturing in vitro.

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