Structural Analysis of Historical Masonry Church Construction

Author(s):  
Łukasz Bednarz ◽  
Artur Górski ◽  
Jerzy Jasieńko ◽  
Eugeniusz Rusiński
2021 ◽  
Vol 6 (4) ◽  
pp. 472
Author(s):  
Giancarlo Ramaglia ◽  
Gian Piero Lignola ◽  
Francesco Fabbrocino ◽  
Andrea Prota

Author(s):  
M. C. Giangregorio ◽  
N. Cortinovis ◽  
G. Cardani ◽  
D. Coronelli

<p><strong>Abstract.</strong> Nowadays, different methods are used to study historical masonry buildings. Among these, for the study of architectures with complex geometry, the effectiveness of an integrated approach, that is a method of analysis combining different disciplines, is increasingly evident.</p><p>The aim of this paper is to show the importance of combining direct observation with structural analysis in order to understand the level of safety in buildings with composite geometries.</p><p>This paper describes the analysis executed in the XX century parish church of San Bernardino in Sesto Calende (Va), which displays serious cracks and damage caused by soil settlements.</p><p>The integrated approach starts with historical analysis, by consulting all available documents and drawings. To understand the geometry of the structure a new survey has been made and a three-dimensional digital representation was modeled, by which better deriving the weight of all the elements in the construction, and to find the correct actions and thrust on arches, columns, bases and foundation. All this data was used in the structural analysis based on the static method of limit analysis. For the material behavior the model proposed by J. Heyman (1966) is used, considering the “no tension” failure criterion. The static theorem of minimum reactions for settled states enunciated by M. Como (2010) is employed for the analysis of soil settlement effects on the building’s response.</p><p>The work shows how direct survey of geometry and damage of a complex building has an effective importance in the structural analysis to ensure Cultural Heritage preservation and safety.</p>


Author(s):  
W. H. Wu ◽  
R. M. Glaeser

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.


Author(s):  
E. Loren Buhle ◽  
Pamela Rew ◽  
Ueli Aebi

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.


Author(s):  
Paul DeCosta ◽  
Kyugon Cho ◽  
Stephen Shemlon ◽  
Heesung Jun ◽  
Stanley M. Dunn

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).


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