The Plasma Membrane Calcium Pump (PMCA): Regulation of Cytosolic Ca2+, Genetic Diversities and Its Role in Sub-plasma Membrane Microdomains

2017 ◽  
pp. 3-21 ◽  
Author(s):  
Joachim Krebs
Keyword(s):  
Plasma Membrane ◽  
Calcium Pump ◽  
Membrane Microdomains ◽  
Plasma Membrane Calcium Pump ◽  
Plasma Membrane Microdomains

Positive charge modifications alter the ability of XIP to inhibit the plasma membrane calcium pump

1996 ◽  
Vol 271 (3) ◽  
pp. C736-C741 ◽  
Author(s):  
W. Xu ◽  
C. Gatto ◽  
M. A. Milanick
Keyword(s):  
Plasma Membrane ◽  
Positive Charge ◽  
Calcium Pump ◽  
Inhibitory Peptide ◽  
Ca Pump ◽  
Contact Points ◽  
Plasma Membrane Calcium Pump ◽  
Peptide Analogues ◽  
Positively Charged

Exchange inhibitory peptide (XIP; RRLLFYKYVYKRYRAGKQRG) is the shortest peptide that inhibits the plasma membrane Ca pump at high Ca (A. Enyedi, T. Vorherr, P. James, D. J. McCormick, A. G. Filoteo, E. Carafoli, and J. T. Penniston, J. Biol. Chem. 264: 12313-12321, 1989). Sulfosuccinimidyl acetate (SNA)-modified XIP does not inhibit the Ca pump; SNA neutralizes the positive charge on Lys at positions 7, 11, and 17. Peptide 2CK-XIP (RRLLFYRYVYRCYCAGRQKG) inhibits the pump, but the iodoacetamido-modified peptide does not inhibit. Three peptide analogues, in which 7, 11, and 17 were Ala, Cys, or Lys, inhibited about as well as XIP. SNA modification of these analogues (each with 1 Lys) did not inhibit. SNA modification of 2CK-XIP results in a peptide that does not inhibit; thus position 19 is important. Our results suggest that it is critical that position 19 be positively charged, that positions 7, 11, and 17 are important contact points between XIP and the Ca pump (with at least one positively charged), and that, whereas it is not essential that residues 12 and 14 be positive, they cannot be negative.

Use of the Polymerase Chain Reaction for the Detection of Alternatively Spliced mRNAs of Plasma Membrane Calcium Pump

1993 ◽  
Vol 12 (5) ◽  
pp. 435-440 ◽  
Author(s):  
FAZLUL H. SARKAR ◽  
DANIEL E. BALL ◽  
WAYNE TSANG ◽  
YI-WEI LI ◽  
TUAN H. KUO
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasma Membrane ◽  
Calcium Pump ◽  
Chain Reaction ◽  
Plasma Membrane Calcium Pump ◽  
Alternatively Spliced ◽  
Polymerase Chain

scholarly journals Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment

2010 ◽  
Vol 191 (4) ◽  
pp. 771-781 ◽  
Author(s):  
Alexander Ludwig ◽  
Grant P. Otto ◽  
Kirsi Riento ◽  
Emily Hams ◽  
Padraic G. Fallon ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Ex Vivo ◽  
Neutrophil Migration ◽  
Neutrophil Recruitment ◽  
Membrane Microdomains ◽  
Neutrophil Chemotaxis ◽  
Myosin Iia ◽  
Plasma Membrane Microdomains ◽  
Cortical Cytoskeleton

We studied the function of plasma membrane microdomains defined by the proteins flotillin 1 and flotillin 2 in uropod formation and neutrophil chemotaxis. Flotillins become concentrated in the uropod of neutrophils after exposure to chemoattractants such as N-formyl-Met-Leu-Phe (fMLP). Here, we show that mice lacking flotillin 1 do not have flotillin microdomains, and that recruitment of neutrophils toward fMLP in vivo is reduced in these mice. Ex vivo, migration of neutrophils through a resistive matrix is reduced in the absence of flotillin microdomains, but the machinery required for sensing chemoattractant functions normally. Flotillin microdomains specifically associate with myosin IIa, and spectrins. Both uropod formation and myosin IIa activity are compromised in flotillin 1 knockout neutrophils. We conclude that the association between flotillin microdomains and cortical cytoskeleton has important functions during neutrophil migration, in uropod formation, and in the regulation of myosin IIa.

scholarly journals Topography of plasma membrane microdomains and its consequences for mast cell signaling

2006 ◽  
Vol 36 (10) ◽  
pp. 2795-2806 ◽  
Author(s):  
Petr Heneberg ◽  
Pavel Lebduška ◽  
L'ubica Dráberová ◽  
Jan Korb ◽  
Petr Dráber
Keyword(s):  
Mast Cell ◽  
Plasma Membrane ◽  
Cell Signaling ◽  
Membrane Microdomains ◽  
Plasma Membrane Microdomains

scholarly journals Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling

2016 ◽  
Vol 18 (9) ◽  
pp. 1251-1267 ◽  
Author(s):  
Christian Kock ◽  
Henning Arlt ◽  
Christian Ungermann ◽  
Jürgen J. Heinisch
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Yeast Cell ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
Membrane Microdomains ◽  
Plasma Membrane Microdomains

scholarly journals DC-SIGN and Influenza Hemagglutinin Dynamics in Plasma Membrane Microdomains Are Markedly Different

2011 ◽  
Vol 100 (11) ◽  
pp. 2662-2670 ◽  
Author(s):  
Michelle S. Itano ◽  
Aaron K. Neumann ◽  
Ping Liu ◽  
Feng Zhang ◽  
Enrico Gratton ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Microdomains ◽  
Influenza Hemagglutinin ◽  
Plasma Membrane Microdomains
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