Inhibitors Around the Antimycin-Sensitive Site in the Respiratory Chain

1969 ◽  
pp. 282-300 ◽  
Author(s):  
Z. Kaniuga ◽  
J. Bryła ◽  
E. C. Slater
Keyword(s):  
Respiratory Chain ◽  
Sensitive Site

scholarly journals Electron transport in mitochondria isolated from the flagellate Polytomella caeca

1968 ◽  
Vol 107 (6) ◽  
pp. 829-837 ◽  
Author(s):  
D Lloyd ◽  
B. Chance
Keyword(s):  
Electron Transport ◽  
Spectral Data ◽  
Respiratory Chain ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Antimycin A ◽  
Fluorescence Emission Spectra ◽  
Sensitive Site ◽  
Kinetics Of ◽  
Transport In Mitochondria

1. Mitochondria isolated from Polytomella caeca contain cytochromes b, c+c1 and a+a3 and several flavoprotein species. 2. Electron transport is inhibited by antimycin A, rotenone, piericidin A and cyanide. 3. Spectral data indicate that antimycin A inhibits the reoxidation of reduced cytochrome b. 4. Various types of flavoprotein are characterized by simultaneous spectrophotometric and fluorimetric measurements on antimycin A-inhibited preparations and also by their absorption and fluorescence-emission spectra. 5. The rotenone-sensitive site lies between the two flavoproteins of the respiratory chain, designated FpD1 and FpD2. 6. Other flavoprotein species detected include those involved in the oxidation of succinate and externally added NADH; a large proportion of mitochondrial flavine is reduced by dithionite but not by known respiratory substrates. 7. The kinetics of flavoprotein and cytochrome reactions were studied.

scholarly journals NADH- and NADPH-dependent formation of superoxide anions by bovine heart submitochondrial particles and NADH–ubiquinone reductase preparation

1979 ◽  
Vol 180 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Koichiro Takeshige ◽  
Shigeki Minakami
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Inorganic Salts ◽  
Superoxide Anions ◽  
Submitochondrial Particles ◽  
Bovine Heart ◽  
Sensitive Site ◽  
Effects Of Ph ◽  
High Concentrations ◽  
O2 Production

1. Both NADH and NADPH supported the oxidation of adrenaline to adrenochrome in bovine heart submitochondrial particles. The reaction was completely inhibited in the presence of superoxide dismutase, suggesting that superoxide anions (O2−) are responsible for the oxidation. The optimal pH of the reaction with NADPH was at pH7.5, whereas that with NADH was at pH9.0. The reaction was inhibited by treatment of the preparation with p-hydroxymercuribenzoate and stimulated by treatment with rotenone. Antimycin A and cyanide stimulated the reaction to the same extent as rotenone. The NADPH-dependent reaction was inhibited by inorganic salts at high concentrations, whereas the NADH-dependent reaction was stimulated. 2. Production of O2− by NADH–ubiquinone reductase preparation (Complex I) with NADH or NADPH as an electron donor was assayed by measuring the formation of adrenochrome or the reduction of acetylated cytochrome c which does not react with the respiratory-chain components. p-Hydroxymercuribenzoate inhibited the reaction and rotenone stimulated the reaction. The effects of pH and inorganic salts at high concentrations on the NADH- and NADPH-dependent reactions of Complex I were essentially similar to those on the reactions of submitochondrial particles. 3. These findings suggest that a region between a mercurialsensitive site and the rotenone-sensitive site of the respiratory-chain NADH dehydrogenase is largely responsible for the NADH- and NADPH-dependent O2− production by the mitochondrial inner membranes.

scholarly journals By-Pass of the Amytal-Sensitive Site of the Respiratory Chain in Mitochondria by Means of Vitamin K3.

1960 ◽  
Vol 14 ◽  
pp. 1840-1842 ◽  
Author(s):  
T. E. Conover ◽  
L. Ernster ◽  
M. Ljunggren ◽  
J. McKinley McKee ◽  
Gertrud Westin
Keyword(s):  
Respiratory Chain ◽  
Vitamin K3 ◽  
Sensitive Site

Molecular diagnosis in mitochondrial respiratory chain deficiency using exome sequencing

2013 ◽  
Vol 44 (02) ◽  
Author(s):  
R Kopajtich ◽  
T Haack ◽  
B Haberberger ◽  
J Mayr ◽  
W Sperl ◽  
...  
Keyword(s):  
Exome Sequencing ◽  
Respiratory Chain ◽  
Molecular Diagnosis ◽  
Mitochondrial Respiratory Chain ◽  
Respiratory Chain Deficiency ◽  
Mitochondrial Respiratory

333-LB: Impact of Aging on Mitochondrial Respiratory Chain Expression and Pancreatic Islet Cell Composition by Using a Mitochondrial DNA Mutator Mouse Model

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 333-LB
Author(s):  
XUEFEI YU ◽  
CATHERINE ARDEN ◽  
CHUN CHEN ◽  
CARLA BRADSHAW ◽  
JULIA WHITEHALL ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Mouse Model ◽  
Respiratory Chain ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Mitochondrial Respiratory Chain ◽  
Pancreatic Islet Cell ◽  
Cell Composition ◽  
Mitochondrial Respiratory

Phương pháp thử nghiệm độc học sử dụng hệ hô hấp của màng ty thể tim bò = Ecotoxicology tests based on the respiratory chain activity of beef heart mitochondrial particles.

2007 ◽  
Vol 10 (1) ◽  
Author(s):  
Nga Phi Lê ◽  
Thư Hiền Thị Nguyễn ◽  
Hoa Liên Thị Nguyễn ◽  
Hương Giang Thị Dương ◽  
Kháng Duy Đinh
Keyword(s):  
Respiratory Chain ◽  
Beef Heart ◽  
Respiratory Chain Activity
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