Full Absolute Stereostructures of Natural Products Directly from Crude Extracts: The HPLC-MS/MS-NMR-CD ‘Triad’

2003 ◽  
pp. 89-116 ◽  
Author(s):  
G. Bringmann ◽  
G. Lang
Keyword(s):  
Natural Products ◽  
Crude Extracts

Discovering New Natural Products Directly from Crude Extracts by HPLC-SPE-NMR:  Chinane Diterpenes inHarpagophytumprocumbens

2006 ◽  
Vol 69 (4) ◽  
pp. 527-530 ◽  
Author(s):  
Cailean Clarkson ◽  
Steen Honoré Hansen ◽  
Peter J. Smith ◽  
Jerzy W. Jaroszewski
Keyword(s):  
Natural Products ◽  
Crude Extracts

scholarly journals In Vitro Antiviral Activity Against Zika Virus From a Natural Product of the Brazilian Brown Seaweed Dictyota menstrualis

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985912 ◽  
Author(s):  
Claudio C. Cirne-Santos ◽  
Caroline de S. Barros ◽  
Max W. L. Gomes ◽  
Rafaela Gomes ◽  
Diana N. Cavalcanti ◽  
...  
Keyword(s):  
Natural Products ◽  
Zika Virus ◽  
Brown Seaweed ◽  
Health Concern ◽  
Dependent Manner ◽  
Dictyota Menstrualis ◽  
Crude Extracts ◽  
High Concentrations ◽  
Inhibitory Potential

Natural products isolated from seaweeds have shown great antiviral potential against numerous viruses such as human type 1 herpes, human immunodeficiency virus, and dengue. Diterpenes produced by the brown seaweeds Dictyota and Canistrocarpus, in particular, have shown antiviral or virucidal activity. Recently, the Zika virus (ZIKV) has become a major public health concern due to its widespread dissemination throughout the Americas. Since no vaccines are available, and no drugs have effectively treated recent cases of infection, our group evaluated products from Dictyota menstrualis for their antiviral potential, alone and in combination with Ribavirin. We first evaluated the compounds’ cytotoxicity at high concentrations, and then evaluated the inhibition of ZIKV replication by crude extracts and acetylated crude extracts and their fractions at 20 μg/mL. The F-6 and FAc-2 fractions, rich in cyclic diterpenes with aldehyde groupings, inhibited ZIKV replication by >74%, with inhibition behaving in a dose-dependent manner and the 50% effective concentration (EC50) values of 2.80 (F-6) and 0.81 (FAc-2) μg/mL. Regarding the mechanism of action, FAc-2 had strong virucidal potential, and F-6 inhibited viral adsorption. Associating FAc-2 with Ribavirin at suboptimal dosages produced a strong synergistic effect that completely inhibited viral replication. Our results indicate that these natural products have excellent inhibitory potential against ZIKV replication and may be promising for developing affective therapies.

scholarly journals Solid-phase enrichment and analysis of electrophilic natural products

2017 ◽  
Vol 13 ◽  
pp. 405-409 ◽  
Author(s):  
Frank Wesche ◽  
Yue He ◽  
Helge B Bode
Keyword(s):  
Natural Products ◽  
Solid Phase ◽  
New Drugs ◽  
Crude Extracts ◽  
Novel Methods

In search for new natural products, which may lead to the development of new drugs for all kind of applications, novel methods are needed. Here we describe the identification of electrophilic natural products in crude extracts via their reactivity against azide as a nucleophile followed by their subsequent enrichment using a cleavable azide-reactive resin (CARR). Using this approach, natural products carrying epoxides and α,β-unsaturated enones as well as several unknown compounds were identified in crude extracts from entomopathogenic Photorhabdus bacteria.

Medicinal plants towards modeling skin cancer

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad El-Harakeh ◽  
Sara Al-Ghadban ◽  
Rémi Safi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Natural Products ◽  
Skin Cancer ◽  
Medicinal Plants ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Chemopreventive Agents ◽  
Melanoma Cancer ◽  
Crude Extracts ◽  
Novel Approach

: Skin cancer remains a major cause of mortality worldwide. It can be divided into melanoma and non-melanoma cancer which comprises mainly squamous cell carcinoma and basal cell carcinoma. Although conventional therapies have ameliorated the management of skin cancer, the search for chemopreventive compounds is still the most effective and safer strategy to treat cancer. Nowadays, chemoprevention is recognized as a novel approach to prevent or inhibit carcinogenesis steps by the use of natural products. Crude extracts of plants and isolated phytocompounds are considered chemopreventive agents since they harbor anti-inflammatory, antioxidant and anti-oncogenic properties against many types of diseases and cancers. In this review, we will discuss the therapeutic effect and preventive potential of selected medicinal plants used as crude extracts or as phytocompounds against melanoma and non-melanoma cutaneous cancers.

scholarly journals Diversity and Bioactive Potential of Actinobacteria Isolated from a Coastal Marine Sediment in Northern Portugal

2020 ◽  
Vol 8 (11) ◽  
pp. 1691
Author(s):  
Inês Ribeiro ◽  
Mariana Girão ◽  
Diogo A. M. Alexandrino ◽  
Tiago Ribeiro ◽  
Chiara Santos ◽  
...  
Keyword(s):  
Natural Products ◽  
Marine Sediment ◽  
Ductal Carcinoma ◽  
Large Fraction ◽  
Bioactive Molecules ◽  
Good Opportunity ◽  
Portuguese Coast ◽  
Northern Portugal ◽  
Crude Extracts ◽  
Bioactive Potential

Natural compounds have had increasing applications in the biotechnological sector, with a large fraction of these substances being channeled to the pharmaceutical industry due to their important pharmacological properties. The discovery of new bioactive molecules with novel mechanisms of action constitutes a promising solution for the design of alternative therapeutic solutions. Actinobacteria are a large group of morphologically and physiologically diverse bacteria well known for their production of biotechnologically relevant compounds. The Portuguese coast is scantly explored in terms of Actinobacteria diversity and respective bioactive potential, offering a good opportunity to find new Actinobacteria taxa and bioactive natural products. In this study, we investigated the Actinobacteria diversity associated with a sediment sample collected from the intertidal zone of a beach in northern Portugal, through a cultivation-dependent approach, and screened its antimicrobial and cytotoxic potential. A total of 52 Actinobacteria strains were recovered from the marine sediment, with the largest fraction of the isolates belonging to the genus Micromonospora. Bioactivity screening assays identified crude extracts of six Streptomyces strains active against C. albicans, exhibiting minimum inhibition concentration (MIC) values in the range of 3.90–125 μg mL−1. Twenty-five Actinobacteria crude extracts (obtained from strains of the genera Micromonospora, Streptomyces and Actinomadura) exhibited significant effects on the viability of at least one tested cancer cell line (breast ductal carcinoma T-47D and liver hepatocellular carcinoma HepG2). The Actinobacteria extracts demonstrating activity in the antimicrobial and/or cytotoxic assays were subjected to metabolomic analysis (Mass spectrometry (MS)-based dereplication and molecular networking analyses), indicating the presence of four clusters that may represent new natural products. The results obtained demonstrate the importance of bioprospecting underexplored environments, like the Portuguese coast, for enhancing the discovery of new natural products, and call attention to the relevance of preserving the natural genetic diversity of coastal environments.

scholarly journals Exploring the Potential of Natural Products From Mangrove Rhizosphere Bacteria as Biopesticides Against Plant Diseases

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2925-2932 ◽  
Author(s):  
Lingyun Hao ◽  
Yu Wang ◽  
Xinqi Chen ◽  
Xiaoli Zheng ◽  
Si Chen ◽  
...  
Keyword(s):  
Natural Products ◽  
Antimicrobial Activities ◽  
Plant Diseases ◽  
Rhizosphere Bacteria ◽  
Vitro Assay ◽  
Bacterial Fermentation ◽  
Crude Extracts ◽  
Acanthus Ilicifolius ◽  
Plant Disease Management

With increasing concerns of the environmental problems associated with current fungicide application, investigation of alternative, environmentally compatible biopesticides for plant disease management is needed. A total of 113 strains associated with Acanthus ilicifolius Linn in the Maipo Reserve, Hong Kong, were isolated and identified. In vitro assay with crude extracts of bacterial fermentation cultures identified ∼26% of the isolates producing antimicrobial compounds against a variety of agriculturally important phytopathogens. Selected crude extracts with inhibition to Colletotrichum fructicola and Magnaporthe oryzae growth significantly suppressed anthracnose and rice blast development in pear fruits and rice plants, respectively, when applied at 50 μg ml−1. Furthermore, 10 of 14 selected crude extracts with good antimicrobial activities had no significant differences in toxicity to the genus Chlorella compared with the control when used at 25 μg ml−1, whereas Amistar Top and Mancozeb completely killed the alga under the same concentration. These data illustrate the potential of natural products from mangrove rhizosphere bacteria in future agricultural application.

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