Phytochemical screening and in vitro antibacterial activity of Syzygium sp.("O-on") crude extracts against Pseudomonas aeruginosa and Staphylococcus aureus

2018 ◽  
Vol 22 (2) ◽  
Author(s):  
Merced Melencion ◽  
◽  
Chris Rey Lituañas ◽  
Andrew Melencion ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Phytochemical Screening ◽  
Crude Extracts ◽  
In Vitro Antibacterial Activity ◽  
And Staphylococcus Aureus

anti-dengue, natural products, Pseudomonas aeruginosa, Staphylococcus aureus

scholarly journals ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 8 (12) ◽  
pp. 262-269
Author(s):  
M. A. Garga ◽  
U. M. Garasin ◽  
M. Abdullahi ◽  
B. A. Muhammed ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Gram Staining ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

scholarly journals Antimicrobial activity of essential oils extracted from leaves of native Moroccan plants against clinical bacterial isolates

10.3823/819 ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Fatima El Malki ◽  
Kamal Eddaraji ◽  
Rajae Alloudane ◽  
Hassane Greche ◽  
Haiat Essalmani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Bactericidal Effect ◽  
Antimicrobial Activities ◽  
Acinetobacter Baumanii ◽  
And Staphylococcus Aureus

Introduction: Medicinal plants are plentiful of bioactive molecules effective against multi-resistance bacteria. The aims of this study were to assess the in vitro antimicrobial activities of essential oils extracted from three Moroccan aromatic plants. Methodology: Analysis of essential oils of Origanum compactum, Rosmarinus officinalis and Pelargonium asperum, collected from different localities in Morocco, were performed using a GC-MS spectrophotometry. Antibacterial activity was evaluated in vitro for five clinical multi-resistant isolates. Results: Origanum showed strong antibacterial activity against tested strains except Pseudomonas aeruginosa while Rosmarinum showed a bactericidal effect against Acinetobacter baumanii, Escherichia coli and Staphylococcus aureus. Pelargonium presented only slight growth inhibition of Staphylococcus aureus on solid medium, but provided bactericidal effect against Acinetobacter baumanii and Staphylococcus aureus. Interestingly, fractions F7 and F8 of Pelargonium which represented only 0.3% and 0.1% of the total mass were found bactericidal respectively against Klebsiella pneumoniae and Pseudomonas aeruginosa. Conclusions: Ours results showed that the antimicrobial activities were variables depending on the chemical composition of essential oils, the fraction used and the microorganism tested.Essential oils fractionation allows detection of bioactive substances, especially those owning antimicrobial activity, present in small quantities.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF THE ETHANOLIC EXTRACT OF JALOH (SALIX TETRASPERMA ROXB.) LEAVES AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA

2018 ◽  
Vol 11 (13) ◽  
pp. 107
Author(s):  
Fitrah Wahyuni ◽  
Urip Harahap ◽  
Masfria Masfria
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Regression Curve ◽  
Significant Difference ◽  
In Vitro Antibacterial Activity ◽  
Weak Antibacterial Activity

Objective: This study aims to determine antibacterial activity of ethanolic extract of jaloh (Salix tetrasperma Roxb.) leaves against Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).Methods: Extract was obtained by maceration method of jaloh (S. tetrasperma Roxb.) leaves dried powder with 96% ethanol as solvent. The antibacterial activities of extract were tested by Kirby–Bauer method against SA and PA. Data were analyzed statistically using Kruskal–Wallis test for significant difference level p<0.05.Results: Based on the regression test, the equation of regression curve of extract antibacterial activity on SA and PA, respectively, was y=350.456x-229.579 and y=331.866x-272.069. The minimum inhibitory concentrations (MICs) of SA and PA from the equation of regression curve, respectively, were 4.5193 and 6.6039 mg/mL.Conclusion: Based on the MIC value, ethanolic jaloh leaves extract had a weak antibacterial activity against SA and PA.

Sign in / Sign up

Export Citation Format

Share Document