Cell-Free Systems for Analysis of Cytoplasmic mRNA Turnover

1997 ◽  
pp. 65-91 ◽  
Author(s):  
C. T. DeMaria ◽  
G. Brewer
Keyword(s):  
Mrna Turnover

A GFP-based assay for monitoring post-transcriptional regulation of ARE-mRNA turnover

2006 ◽  
Vol 2 (11) ◽  
pp. 561 ◽  
Author(s):  
Don Benjamin ◽  
Marco Colombi ◽  
Georg Stoecklin ◽  
Christoph Moroni
Keyword(s):  
Transcriptional Regulation ◽  
Mrna Turnover ◽  
Post Transcriptional Regulation

Regulation of mRNA turnover in cystic fibrosis lung disease

2016 ◽  
Vol 8 (4) ◽  
pp. e1408
Author(s):  
Roopa Biswas ◽  
Parameet Kumar ◽  
Harvey B. Pollard
Keyword(s):  
Cystic Fibrosis ◽  
Lung Disease ◽  
Cystic Fibrosis Lung ◽  
Mrna Turnover ◽  
Cystic Fibrosis Lung Disease

Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure

1993 ◽  
Vol 13 (8) ◽  
pp. 4826-4835
Author(s):  
C L Hsu ◽  
A Stevens
Keyword(s):  
Full Length ◽  
Yeast Cells ◽  
Mrna Turnover ◽  
Turnover Rates ◽  
Wild Type ◽  
Mrna Species ◽  
Turnover Process ◽  
Hydrolysis Of ◽  
Extension Analysis ◽  
Wild Type Yeast

Analysis of the slowed turnover rates of several specific mRNA species and the higher cellular levels of some of these mRNAs in Saccharomyces cerevisiae lacking 5'-->3' exoribonuclease 1 (xrn1 cells) has led to the finding that these yeast contain higher amounts of essentially full-length mRNAs that do not bind to oligo(dT)-cellulose. On the other hand, the length of mRNA poly(A) chains found after pulse-labeling of cells lacking the exoribonuclease, the cellular rate of synthesis of oligo(dT)-bound mRNA, and the initial rate of its deadenylation appeared quite similar to the same measurements in wild-type yeast cells. Examination of the 5' cap structure status of the poly(A)-deficient mRNAs by comparative analysis of the m7G content of poly(A)- and poly(A)+ RNA fractions of wild-type and xrn1 cells suggested that the xrn1 poly(A)- mRNA fraction is low in cap structure content. Further analysis of the 5' termini by measurements of the rate of 5'-->3' exoribonuclease 1 hydrolysis of specific full-length mRNA species showed that approximately 50% of the xrn1 poly(A)-deficient mRNA species lack the cap structure. Primer extension analysis of the 5' terminus of ribosomal protein 51A (RP51A) mRNA showed that about 30% of the poly(A)-deficient molecules of the xrn1 cells are slightly shorter at the 5' end. The finding of some accumulation of poly(A)-deficient mRNA species partially lacking the cap structure together with the reduction of the rate of mRNA turnover in cells lacking the enzyme suggest a possible role for 5'-->3' exoribonuclease 1 in the mRNA turnover process.

scholarly journals Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis

2015 ◽  
Vol 11 (10) ◽  
pp. e1005196 ◽  
Author(s):  
Jian Ye ◽  
Junyi Yang ◽  
Yanwei Sun ◽  
Pingzhi Zhao ◽  
Shiqiang Gao ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Mrna Turnover

scholarly journals Multiplexed gene control reveals rapid mRNA turnover

2017 ◽  
Vol 3 (7) ◽  
pp. e1700006 ◽  
Author(s):  
Antoine Baudrimont ◽  
Sylvia Voegeli ◽  
Eduardo Calero Viloria ◽  
Fabian Stritt ◽  
Marine Lenon ◽  
...  
Keyword(s):  
Mrna Turnover ◽  
Gene Control

scholarly journals Rapid transit in the immune cells: the role of mRNA turnover regulation

2007 ◽  
Vol 81 (6) ◽  
pp. 1335-1344 ◽  
Author(s):  
Khalid S. A. Khabar
Keyword(s):  
Immune Cells ◽  
Mrna Turnover ◽  
Rapid Transit

scholarly journals Adhesion-dependent regulation of an A+U-rich element-binding activity associated with AUF1.

1997 ◽  
Vol 17 (7) ◽  
pp. 3898-3906 ◽  
Author(s):  
O I Sirenko ◽  
A K Lofquist ◽  
C T DeMaria ◽  
J S Morris ◽  
G Brewer ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Rna Binding ◽  
Kinase Inhibitor ◽  
Binding Activity ◽  
P38 Map Kinase ◽  
Mrna Turnover ◽  
Mrna Stabilization ◽  
Rna Probes ◽  
Mediators Of Inflammation ◽  
Monocyte Adherence

Monocyte adherence results in the rapid transcriptional activation and mRNA stabilization of numerous mediators of inflammation and tissue repair. While the enhancer and promoter elements associated with transcriptional activation have been studied, mechanisms linking adhesion, mRNA stabilization, and translation are unknown. GROalpha and interleukin-1beta (IL-1beta) mRNAs are highly labile in nonadhered monocytes but stabilize rapidly after adherence. GROalpha and IL-1beta transcripts both contain A+U-rich elements (AREs) in the 3' untranslated region (UTR) which have been directly associated with rapid mRNA turnover. To determine if the GROalpha ARE region was recognized by factors associated with mRNA degradation, we carried out mobility gel shift analyses using a series of RNA probes encompassing the entire GROalpha transcript. Stable complexes were formed only with the proximal 3' UTR which contained the ARE region. The two slower-moving complexes were rapidly depleted following monocyte adherence but not direct integrin engagement. Deadherence reactivated the two largest ARE-binding complexes and destabilized IL-1beta transcripts. Antibody supershift studies demonstrated that both of these ARE RNA-binding complexes contained AUF1. The formation of these complexes and the accelerated mRNA turnover are phosphorylation-dependent events, as both are induced in adherent monocytes by the tyrosine kinase inhibitor genistein and the p38 MAP kinase inhibitor of IL-1beta translation, SK&F 86002. These results demonstrate that cell adhesion and deadhesion rapidly and reversibly modify both cytokine mRNA stability and the RNA-binding complexes associated with AUF1.

mRNA Turnover

2006 ◽  
Author(s):  
Philip Mitchell
Keyword(s):  
Mrna Turnover
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