Appearance of the T-cell marker CD8 on B chronic lymphatic leukemia cells in long-term cultures

1991 ◽  
Vol 34 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Ruth Farkas ◽  
Shlomo Ben-Efraim ◽  
Yosef Manor ◽  
Israel Zan-Bar ◽  
Abraham Klajman
Keyword(s):  
T Cell ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Cell Marker ◽  
Lymphatic Leukemia ◽  
Lymphatic Leukemia Cells

scholarly journals Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 723-736 ◽  
Author(s):  
E Thiel ◽  
H Rodt ◽  
D Huhn ◽  
S Thierfelder
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Sheep Erythrocyte ◽  
T Antigen ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Lymphatic Leukemia ◽  
Clonal Origin ◽  
Lymphatic Leukemia Cells

Abstract B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

scholarly journals Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 723-736
Author(s):  
E Thiel ◽  
H Rodt ◽  
D Huhn ◽  
S Thierfelder
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Sheep Erythrocyte ◽  
T Antigen ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Lymphatic Leukemia ◽  
Clonal Origin ◽  
Lymphatic Leukemia Cells

B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study

1989 ◽  
Vol 1 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Davide Soligo ◽  
Giancarlo Balercia ◽  
Francesco Osculati ◽  
Nadia Quirici ◽  
Andrea Sbarbati ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Leukemia Cells ◽  
Surface Phenotype ◽  
Lymphatic Leukemia ◽  
Lymphatic Leukemia Cells

scholarly journals Cytotoxic Polyketide Metabolites from a Marine Mesophotic Zone Chalinidae Sponge-Associated Fungus Pleosporales sp. NBUF144

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 186
Author(s):  
Jing Zhou ◽  
Hairong Zhang ◽  
Jing Ye ◽  
Xingxin Wu ◽  
Weiyi Wang ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Leukemia Cells ◽  
Ionization Mass ◽  
X Ray Diffraction ◽  
X Ray ◽  
Lymphatic Leukemia ◽  
Ic50 Value ◽  
Lymphatic Leukemia Cells

Two new polyketide natural products, globosuxanthone F (1), and 2′-hydroxy bisdechlorogeodin (2), were isolated from the fungus Pleosporales sp. NBUF144, which was derived from a 62 m deep Chalinidae family sponge together with four known metabolites, 3,4-dihydroglobosuxanthone A (3), 8-hydroxy-3-methylxanthone-1-carboxylate (4), crosphaeropsone C (5), and 4-megastigmen-3,9-dione (6). The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR and high-resolution electrospray ionization mass spectra (HRESIMS) data. The absolute configuration of 1 was further established by single-crystal X-ray diffraction studies. Compounds 1-5 were evaluated for cytotoxicity towards CCRF-CEM human acute lymphatic leukemia cells, and it was found that 1 had an IC50 value of 0.46 µM.

scholarly journals Subcellular Distribution of Human Leukocytic Cathepsins

Blood ◽  
1968 ◽  
Vol 32 (1) ◽  
pp. 119-133 ◽  
Author(s):  
MARY ANN STILES ◽  
JANE FRAENKEL-CONRAT
Keyword(s):  
Specific Activity ◽  
Soluble Fraction ◽  
Leukemia Cells ◽  
Nuclear Fraction ◽  
Lymphatic Leukemia ◽  
Specific Activities ◽  
Mixed Populations ◽  
Peripheral Leukocytes ◽  
Lymphatic Leukemia Cells ◽  
Fraction I

Abstract 1. Methods are presented for the disruption of human peripheral leukocytes by sonication and the separation of the released particulate matter by differential centrifugation. 2. The distribution of catheptic activity in the various fractions was investigated both on material obtained from mixed populations of leukocytes and on that from isolated lymphocytes and granulocytes. 3. All of the leukocytic fractions tested contained cathepsins active at pH 3.5 and pH 8.5. 4. At pH 3.5, the catheptic activity was rather evenly distributed among nuclear (I), and light (IV) and heavy (II) granular fractions, with practically no activity in the soluble fraction (V). 5. At pH 8.5, there was always more catheptic activity in the heavier (II) than the lighter (IV) granules, variable amounts in the nuclear fraction (I) and very little in the supernatant (V). 6. At pH 3.5, the lymphocytic granules showed considerably higher proteolytic specific activity than those from granulocytes. 7. At pH 8.5, all fractions except the heavy granules (II) had higher proteolytic specific activities in granulocytes than in lymphocytes. 8. At pH 3.5, and to a lesser extent at pH 8.5, the light (IV) and heavy (II) granules from chronic lymphatic leukemia cells contained much lower cathepic activity than those from normal lymphocytes. 9. Cathepsins in each subcellular fraction are very labile, losing as much as 80 per cent of their activity upon storage at 4 C. overnight in distilled water.

Sign in / Sign up

Export Citation Format

Share Document