Potential Role of Silicon-Oxygen Radicals in Acute Lung Injury

Acute lung injury (ALI) is associated with histopathological diffuse alveolar damage. The potential role of mesenchymal stem cells (MSCs) in the treatment of various clinical disorders have been widely documented, such as those for ALI. Recent evidence has demonstrated that exosomes from endothelial progenitor cells can improve outcomes of the lipopolysaccharide (LPS)-induced ALI. However, there has been no research on the potential role of MSC-exosomes in the treatment of sepsis-induced ALI, which is worth further exploration. Thus, the objective of our study was to identify whether the MSC-exosomes could reverse ALI. The ALI model induced by LPS was established in this study. MTT assay was performed to test cell proliferation. Expression of inflammatory factors (TNF-α, IL-6, and IL-10) in the LPS-treated type II alveolar epithelial cells (AECs) (MLE-12) was detected by ELISA. After co-culture of MSC-exosomes with LPS-treated MLE-12 cells, we found that the cell proliferation of MLE-12 cells gradually increased. Furthermore, we selected five of the Nrf-2/ARE- and NF-κB signaling pathway-related genes to explore if MSC-exosomes could reverse LPS-induced ALI through Nrf-2/ARE and NF-κB signaling pathways. QRT-PCR and western blot experiment results showed that the expression of these five genes were significantly regulated after stimulation with high-concentration LPS and exosome intervention. Taken together, these findings highlighted the fact that MSC-exosomes could reverse ALI through the Nrf-2/ARE and NF-κB signaling pathways. The MSC-exosome may be the potential future therapeutic strategy for the treatment of ALI.

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Acute lung injury and the coagulation pathway: potential role of gene polymorphisms in the protein C and fibrinolytic pathways

Intensive Care Medicine ◽

10.1007/s00134-006-0223-5 ◽

2006 ◽

Vol 32 (9) ◽

pp. 1293-1303 ◽

Cited By ~ 28

Author(s):

Anil Sapru ◽

Joseph L. Wiemels ◽

John S. Witte ◽

Lorraine B. Ware ◽

Michael A. Matthay

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Gene Polymorphisms ◽

Protein C ◽

Potential Role ◽

Coagulation Pathway

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Potential Role of Adaptive Immune Cells in Acute Lung Injury.

10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5674 ◽

2009 ◽

Author(s):

CJ Suarez ◽

T Nakajima ◽

KY Jen ◽

KW Lin ◽

S Makani ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Immune Cells ◽

Potential Role ◽

Adaptive Immune ◽

Adaptive Immune Cells

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The Immune System in Transfusion-Related Acute Lung Injury Prevention and Therapy: Update and Perspective

Frontiers in Molecular Biosciences ◽

10.3389/fmolb.2021.639976 ◽

2021 ◽

Vol 8 ◽

Author(s):

Kai Guo ◽

Shuxuan Ma

Keyword(s):

Acute Lung Injury ◽

Immune System ◽

Lung Injury ◽

Therapeutic Strategy ◽

Potential Role ◽

Physical Trauma ◽

Immune System Activation ◽

Prevention And Treatment ◽

System Activation

As an initiator of respiratory distress, transfusion-related acute lung injury (TRALI) is regarded as one of the rare complications associated with transfusion medicine. However, to date, the pathogenesis of TRALI is still unclear, and specific therapies are unavailable. Understanding the mechanisms of TRALI may promote the design of preventive and therapeutic strategies. The immune system plays vital roles in reproduction, development and homeostasis. Sterile tissue damage, such as physical trauma, ischemia, or reperfusion injury, induces an inflammatory reaction that results in wound healing and regenerative mechanisms. In other words, in addition to protecting against pathogens, the immune response may be strongly associated with TRALI prevention and treatment through a variety of immunomodulatory strategies to inhibit excessive immune system activation. Immunotherapy based on immune cells or immunological targets may eradicate complications. For example, IL-10 therapy is a promising therapeutic strategy to explore further. This review will focus on ultramodern advances in our understanding of the potential role of the immune system in TRALI prevention and treatment.

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