Radiation-Induced Formation of DNA Double-Strand Breaks in Plasmids and E. coli

1994 ◽  
pp. 1-9 ◽  
Author(s):  
D. Schulte-Frohlinde
Keyword(s):  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
E Coli ◽  
Radiation Induced

scholarly journals Evolution of Chi motifs in Proteobacteria

2021 ◽  
Author(s):  
Angélique Buton ◽  
Louis-Marie Bobay
Keyword(s):  
Sequence Similarity ◽  
Bacterial Species ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Large Dataset ◽  
High Sequence Similarity ◽  
Strand Breaks ◽  
E Coli ◽  
Dna Motif ◽  
And Staphylococcus Aureus

Abstract Homologous recombination is a key pathway found in nearly all bacterial taxa. The recombination complex allows bacteria to repair DNA double strand breaks but also promotes adaption through the exchange of DNA between cells. In Proteobacteria, this process is mediated by the RecBCD complex, which relies on the recognition of a DNA motif named Chi to initiate recombination. The Chi motif has been characterized in Escherichia coli and analogous sequences have been found in several other species from diverse families, suggesting that this mode of action is widespread across bacteria. However, the sequences of Chi-like motifs are known for only five bacterial species: E. coli, Haemophilus influenzae, Bacillus subtilis, Lactococcus lactis and Staphylococcus aureus. In this study we detected putative Chi motifs in a large dataset of Proteobacteria and we identified four additional motifs sharing high sequence similarity and similar properties to the Chi motif of E. coli in 85 species of Proteobacteria. Most Chi motifs were detected in Enterobacteriaceae and this motif appears well conserved in this family. However, we did not detect Chi motifs for the majority of Proteobacteria, suggesting that different motifs are used in these species. Altogether these results substantially expand our knowledge on the evolution of Chi motifs and on the recombination process in bacteria.

Role for Artemis nuclease in the repair of radiation-induced DNA double strand breaks by alternative end joining

DNA Repair ◽  
2015 ◽  
Vol 31 ◽  
pp. 29-40 ◽  
Author(s):  
Mario Moscariello ◽  
Radi Wieloch ◽  
Aya Kurosawa ◽  
Fanghua Li ◽  
Noritaka Adachi ◽  
...  
Keyword(s):  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
End Joining ◽  
Strand Breaks ◽  
Alternative End Joining ◽  
Radiation Induced

scholarly journals Quantitative, Noninvasive Imaging of Radiation-Induced DNA Double-Strand Breaks In Vivo

2011 ◽  
Vol 71 (12) ◽  
pp. 4130-4137 ◽  
Author(s):  
Wenrong Li ◽  
Fang Li ◽  
Qian Huang ◽  
Jingping Shen ◽  
Frank Wolf ◽  
...  
Keyword(s):  
Noninvasive Imaging ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Radiation Induced
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