Resonance Isolation and Identification Method

1993 ◽  
pp. 83-97
Author(s):  
Naum D. Veksler ◽  
Herbert Überall
Keyword(s):  
Isolation And Identification ◽  
Identification Method

Isolation and Identification of Enteropathogenic Campylobacter spp. from Chicken Samples in Taipei

2000 ◽  
Vol 63 (3) ◽  
pp. 304-308 ◽  
Author(s):  
DANIEL YANG-CHIH SHIH
Keyword(s):  
Selective Medium ◽  
Species Level ◽  
Retail Market ◽  
Isolation And Identification ◽  
Retail Markets ◽  
Selective Media ◽  
Genus Level ◽  
Identification Method ◽  
Test Kit ◽  
Campylobacter Spp

A total of 95 chicken samples that consisted of 34 whole chickens, 32 organs (gizzards and livers), and 29 chicken parts (drumsticks, wings, and breasts), collected from traditional retail markets (no chilling facilities) and supermarkets in Taipei, were examined for the occurrence of enteropathogenic campylobacters. Three selective media, Peterz's charcoal cefoperazone deoxycholate agar, Campy-Cefex agar, and charcoal-based selective medium, were evaluated for their efficacy to isolate Campylobacter spp. from chicken samples. The results showed that there were no differences among the three media to isolate Campylobacter spp. from all chicken samples (P > 0.05). However, there were markedly different isolation rates of campylobacters between supermarket and retail market (P < 0.05). Enteropathogenic campylobacters (C. jejuni and C. coli) were found on 68% of whole chickens, 100% of chicken parts, and 100% of organs from retail markets. In supermarkets, the isolation rates of these campylobacters from whole chickens, chicken parts, and organs were 42%, 53%, and 60%, respectively. The low isolation rates of the two campylobacters isolated from chicken samples in supermarkets differed statistically from those obtained from traditional retail markets (P < 0.10). The API CAMPY test kit also was evaluated for the identification of Campylobacter spp. as compared with the conventional identification method. The results showed that the API CAMPY test kit (Biomerieux, Marcy-l'Etoile, France) could efficiently detect 87 Campylobacter spp. isolates from chicken samples examined, with 100% agreement at the genus level to 94% at the species level as compared with conventional methods.

A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic nanoparticles and MS

PROTEOMICS ◽  
2009 ◽  
Vol 9 (18) ◽  
pp. 4416-4420 ◽  
Author(s):  
Liyan Zhao ◽  
Yangjun Zhang ◽  
Junying Wei ◽  
Dong Cao ◽  
Kehui Liu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Isolation And Identification ◽  
Rapid Isolation ◽  
Identification Method

Some physiological characteristics of a phosphate-removing bacterium, microlunatus phosphovorus, and a simplified isolation and identification method for phosphate-removing bacteria

1998 ◽  
Vol 38 (1) ◽  
pp. 149-157 ◽  
Author(s):  
Y. Ubukata ◽  
S. Takii
Keyword(s):  
Growth Phase ◽  
Stationary Growth Phase ◽  
Physiological Characteristics ◽  
Exponential Growth Phase ◽  
Bacterial Cells ◽  
Stationary Growth ◽  
Isolation And Identification ◽  
Identification Method ◽  
Energy Storage Material ◽  
Aerobic And Anaerobic

Some physiological characteristics of a phosphate (Pi)-removing bacterium, Microlunatus phosphovorus, are investigated using aerobically grown cells and cells exhibiting excess Pi accumulation (EPA) in order to determine a simplified isolation and identification method for other Pi-removing bacteria. Such a method would save on the amount of sterile equipment needed, and reduce the number of experimental steps and labor time. The EPA activity of the isolate reached a plateau 13 hours into the anaerobic incubation time, but it reached 70% of that level after only 5 hours. The EPA activity of the cells during the stationary growth phase was higher than that during the exponential growth phase. Polyphosphate (polyP) accumulated in the cells was shown to be used as an energy storage material (a phosphagen) under both aerobic and anaerobic conditions. During aerobic starvation, the rate of decrease in the ATP concentration of the suspension of cells that contained polyP was markedly less than that of the suspension of cells without polyP. Therefore, bacterial cells rich in polyP survive longer than bacterial cells lacking polyP.

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification
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