Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

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Isolation and Identification of the Anti-Oxidant Constituents from Loropetalum chinense (R. Brown) Oliv. Based on UHPLC–Q-TOF-MS/MS

Molecules ◽

10.3390/molecules23071720 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1720 ◽

Cited By ~ 8

Author(s):

Haifang Chen ◽

Mulan Li ◽

Chen Zhang ◽

Wendi Du ◽

Haihua Shao ◽

...

Keyword(s):

Chemical Composition ◽

Gallic Acid ◽

Protocatechuic Acid ◽

Chemical Constituents ◽

Ethanol Extract ◽

Control Group ◽

Ethyl Gallate ◽

Isolation And Identification ◽

Caffeoylquinic Acid ◽

Tof Ms

The aim of this study was to identify the chemical constituents of Loropetalum chinense (R. Brown) Oliv. (LCO) and determine which of these had antioxidant effects. The chemical composition of a 70% ethanol extract of LCO was analyzed systematically using UHPLC–Q-TOF-MS/MS. The chemical components of the 70% ethanol extract of LCO were then separated and purified using macroporous resin and chromatographic techniques. Antioxidant activity was evaluated using a DPPH assay. In total, 100 compounds were identified tentatively, including 42 gallic acid tannins, 49 flavones, and 9 phenolic compounds. Of these, 7 gallium gallate, 4 flavonoid and 8 quinic acid compounds were separated and purified from the 70% ethanol extract of LCO. The compounds identified for the first time in LCO and in the genus Loropetalum were 3,4,5-trimethoxyphenyl-(6′-O-galloyl)-O-β-d-glucopyranoside, protocatechuic acid, ethyl gallate, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3,5-O-diocaffeoylquinic acid, 4,5-O-diocaffeoylquinic acid and 3,4-O-diocaffeoylquinic acid. The 50% inhibitory concentration (IC50) values of compounds 1,2,3,4,6-penta-O-galloyl-β-d-glucose, gallic acid, protocatechuic acid, and ethyl gallate were 1.88, 1.05, 1.18, and 1.05 μg/mL, respectively. Compared with the control group (VC) (2.08 μg/mL), these compounds exhibited stronger anti-oxidation activity. This study offered considerable insight into the chemical composition of LCO, with preliminary identification of the antioxidant ingredients.

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Acetophenones Isolated from Acronychia Pedunculata and their Anti-proliferative Activities

Natural Product Communications ◽

10.1177/1934578x1601100125 ◽

2016 ◽

Vol 11 (1) ◽

pp. 1934578X1601100

Author(s):

Chihiro Ito ◽

Takuya Matsui ◽

Yoshiaki Ban ◽

Tian-Shung Wu ◽

Masataka Itoigawa

Keyword(s):

Cell Lines ◽

Chemical Constituents ◽

Leukemia Cell ◽

Human Leukemia ◽

Isolation And Identification ◽

Human Leukemia Cell ◽

Leukemia Cell Lines

Study of the chemical constituents of Acronychia pedunculata (L.) Miq. (Rutaceae) stems collected in Taiwan led to the isolation and identification of eight known and three new acetophenones, named acrophenone A (1), B (2), and C (3). Of them, acrovestone (5), acropyrone (6) and acrovestenol (7), which are dimer compounds, strikingly inhibited the proliferation of human leukemia cell lines.

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Rotenoid Derivatives from Derris Trifoliata with Nitric Oxide Production Inhibitory Activity

Natural Product Communications ◽

10.1177/1934578x1200701117 ◽

2012 ◽

Vol 7 (11) ◽

pp. 1934578X1200701

Author(s):

Chihiro Ito ◽

Tomiyasu Murata ◽

Hugh T.-W. Tan ◽

Norio Kaneda ◽

Hiroshi Furukawa ◽

...

Keyword(s):

Nitric Oxide ◽

Inhibitory Activity ◽

Chemical Constituents ◽

Interferon Γ ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Nitric Oxide Production ◽

Murine Macrophage ◽

Isolation And Identification ◽

Oxide Production

Study of the chemical constituents of the stems of Derris trifoliata Lour. (Leguminosae) collected in Singapore led to the isolation and identification of three known and two new rotenoid derivatives. The new derivatives, named derrisfolin A (1) and B (2), inhibited nitric oxide production in murine macrophage-like RAW 264.7 cells stimulated with interferon-γ and lipopolysaccharide.

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Isolation and Identification of some Chemical Constituents ofParmelia nepalensis

Planta Medica ◽

10.1055/s-2006-957822 ◽

1996 ◽

Vol 62 (01) ◽

pp. 93-94 ◽

Cited By ~ 10

Author(s):

Sunil Kumar ◽

Arjun Banskota ◽

Mangala Manandhar

Keyword(s):

Chemical Constituents ◽

Isolation And Identification

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Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

Journal of Analytical Methods in Chemistry ◽

10.1155/2020/8889607 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Guangqiang Huang ◽

Jie Liang ◽

Xiaosi Chen ◽

Jing Lin ◽

Jinyu Wei ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Chemical Constituents ◽

Relevant Literature ◽

Ultra Performance Liquid Chromatography ◽

Negative Ion ◽

Volatile Components ◽

Isolation And Identification ◽

Fragmentation Patterns ◽

Nitrogen Containing Compounds

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

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CHEMICAL CONSTITUENTS OF THE RHIZOMES OF Zingiber collinsii Mood &Theilade (ZINGIBERACEAE) GROWING IN VIETNAM

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/54/4a/12005 ◽

2018 ◽

Vol 54 (4A) ◽

pp. 283

Author(s):

Le Thi My Chau

Keyword(s):

Chemical Constituents ◽

Chemical Investigation ◽

Isolation And Identification ◽

Hydroxymethyl Furfural ◽

Chemical Structures

A chemical investigation of the rhizomes of Zingiber collinsii Mood & Theilade (Zingiberaceae) resulted in the isolation and identification of seven compounds, including a sesquiterpenoid (zerumbone(1)), acoumarin (scopoletin (2)), two flavonoids (quercetin (3), rutin (4)),afurfural derivative (5-(hydroxymethyl)furfural (5)), and two curcuminoids (bisdemethoxycurcumin (6),demethoxycurcumin (7)). The chemical structures of the seven compounds were determined on the basis of NMR and MS analyses.

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