CPC driven isolation and identification of Pistacia vera pericarp secondary metabolites: New source of rare and biologically active triterpenic acids

2017 ◽  
Author(s):  
J Popp ◽  
E Petrakis ◽  
A Angelis ◽  
I Pisimisi ◽  
M Halabalaki ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Biologically Active ◽  
Pistacia Vera ◽  
Isolation And Identification ◽  
Triterpenic Acids

scholarly journals Phytochemical study of the genus amphoricarpos

2021 ◽  
pp. 83-83
Author(s):  
Iris Djordjevic ◽  
Mirjana Cvetkovic ◽  
Milka Jadranin ◽  
Srdjan Bojovic ◽  
Boban Andjelkovic ◽  
...  
Keyword(s):  
Organic Chemistry ◽  
Herbal Medicine ◽  
Secondary Metabolites ◽  
Folk Medicine ◽  
Plant Diseases ◽  
Biologically Active ◽  
Early 20Th Century ◽  
Isolation And Identification ◽  
Plant Adaptation ◽  
Phytochemical Study

Phytochemistry deals with the study of secondary metabolites produced by plants that synthesize these compounds for many reasons, including their own protection against attack of herbivores and plant diseases. Secondary metabolites are believed to represent plant adaptation to various environmental factors and that they enabled the survival of the species. Secondary metabolites of plants can have curative or toxic effects in humans and animals. Herbal medicine has a long tradition in folk medicine and until the early 20th century, when synthetic organic chemistry began to develop, plants were the main source of medicines. Basic goals of our phytochemical research include the following: isolation and identification of new (biologically active) compounds - potential drugs, and chemotaxonomy (chemosystematics). In the following text through one selected example - genus Amphoricarpos Vis. - both aspects of our phytochemical research are shown.

scholarly journals Target screening of plant secondary metabolites in river waters by liquid chromatography coupled to high-resolution mass spectrometry (LC–HRMS)

2020 ◽  
Vol 32 (1) ◽  
Author(s):  
Mulatu Yohannes Nanusha ◽  
Martin Krauss ◽  
Carina D. Schönsee ◽  
Barbara F. Günthardt ◽  
Thomas D. Bucheli ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Surface Waters ◽  
High Resolution Mass Spectrometry ◽  
Water Cycle ◽  
Endocrine Disrupting Chemicals ◽  
Plant Secondary Metabolites ◽  
Biologically Active ◽  
Plant Metabolites ◽  
River Waters ◽  
Target Screening

Abstract Background Substantial efforts have been made to monitor potentially hazardous anthropogenic contaminants in surface waters while for plant secondary metabolites (PSMs) almost no data on occurrence in the water cycle are available. These metabolites enter river waters through various pathways such as leaching, surface run-off and rain sewers or input of litter from vegetation and might add to the biological activity of the chemical mixture. To reduce this data gap, we conducted a LC–HRMS target screening in river waters from two different catchments for 150 plant metabolites which were selected from a larger database considering their expected abundance in the vegetation, their potential mobility, persistence and toxicity in the water cycle and commercial availability of standards. Results The screening revealed the presence of 12 out of 150 possibly toxic PSMs including coumarins (bergapten, scopoletin, fraxidin, esculetin and psoralen), a flavonoid (formononetin) and alkaloids (lycorine and narciclasine). The compounds narciclasine and lycorine were detected at concentrations up to 3 µg/L while esculetin and fraxidin occurred at concentrations above 1 µg/L. Nine compounds occurred at concentrations above 0.1 µg/L, the Threshold for Toxicological Concern (TTC) for non-genotoxic and non-endocrine disrupting chemicals in drinking water. Conclusions Our study provides an overview of potentially biologically active PSMs in surface waters and recommends their consideration in monitoring and risk assessment of water resources. This is currently hampered by a lack of effect data including toxicity to aquatic organisms, endocrine disruption and genotoxicity and demands for involvement of these compounds in biotesting.

scholarly journals NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

2017 ◽  
Vol 9 (2) ◽  
pp. 166 ◽  
Author(s):  
Sayed A. El-toumy ◽  
Joslin Y. Salib ◽  
Nabila H. Shafik ◽  
Asmaa S. Abd Elkarim ◽  
Gihan A. Mick
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Secondary Metabolites ◽  
Gallic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Dpph Method ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>The current study was to deal the isolation and identification of secondary metabolites from <em>Polygonum equisetiforme</em> and evaluation of antioxidant activity of its extract.</p><p><strong>Methods: </strong>The methanol-water extract (7:3) of the air-dried aerial parts of <em>Polygonum equisetiforme</em> was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for <em>in vitro</em> antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.</p><p><strong>Results: </strong>Ten secondary metabolites were isolated from <em>Polygonum equisetiforme</em> in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-<em>O-β</em>-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-<em>O-β</em>-D-glucucorinde methyl ester (4), quercetin 3-<em>O-</em>β-D-glucopyranoside (5), quercetin 7-<em>O-β-</em>D-glucopyranoside (6),<em> </em>quercetin(7)<sub>, </sub>myricetin (8), <em>P</em><sub>-</sub>methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of <em>P. equisetiforme</em> extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC<sub>50 </sub>= 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.</p><p><strong>Conclusion: </strong><em>Polygonum equisetiforme</em> is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.</p>

scholarly journals Studies on the Chemical Diversities of Secondary Metabolites Produced by Neosartorya fischeri via the OSMAC Method

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2772 ◽  
Author(s):  
You-Min Ying ◽  
Lu Huang ◽  
Ting Tian ◽  
Cui-Yu Li ◽  
Shi-Lei Wang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cancer Cell Line ◽  
2D Nmr ◽  
Natural Occurrence ◽  
Fungal Metabolites ◽  
Isolation And Identification ◽  
Late Step ◽  
Neosartorya Fischeri ◽  
The One ◽  
First Time

The One Strain Many Compounds (OSMAC) method was applied to explore the chemical diversities of secondary metabolites produced by Neosartorya fischeri NRRL 181. Four pyripyropenes 1–4, eight steroids 5–11, and four prenylated indole alkaloids 12–15, were obtained from the fungus cultured in petri dishes containing potato dextrose agar (PDA). 1,7,11-trideacetylpyripyropene A (1) and 1,11-dideacetyl pyripyropene A (2) were obtained and spectroscopically characterized (1D, 2D NMR, and HR-ESI-MS) from a natural source for the first time. It offered a sustainable source of these two compounds, which were usually used as starting materials in preparing pyripyropene derivatives. In addition, as compared with all the other naturally occurring pyripyropenes, 1 and 2 possessed unique acetylation patterns that did not follow the established late-step biosynthetic rules of pyripyropenes. The natural occurrence of 1 and 2 in the fungus implied that the timing and order of hydroxylation and acetylation in the late-step biosynthetic pathway of pyripyropenes remained to be revealed. The isolation and identification of 1–15 indicated that the OSMAC method could remarkably alter the metabolic profile and enrich the chemical diversities of fungal metabolites. Compounds 1–4 exhibited no obvious cytotoxicity against the triple-negative breast cancer cell line MDA-MB-231 as compared with taxol.

scholarly journals Urgensi dan Mekanisme Biosintesis Metabolit Sekunder Mikroba Laut

2012 ◽  
Vol 10 (2) ◽  
pp. 120 ◽  
Author(s):  
Risa Nofiani
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Metabolic Pathways ◽  
Abiotic Factors ◽  
Biologically Active ◽  
Secondary Metabolite Production ◽  
Marine Microorganism ◽  
Metabolite Production ◽  
Biotic And Abiotic Factors ◽  
Living Materials

Marine microorganism is one of biologically active potential resources of secondary metabolites. Its potency areso promising that the knowledge of how its secondary metabolite occured need to be studied and collected. Thoseknowledges will enable further study is improving secondary metabolite production in the laboratory. In nature,secondary metabolites synthesis occur when there are effect of both biotic and abiotic factors such as sea waterand microbe symbiosis with other living materials. When this is explained in metabolic pathways, secondarymetabolite synthesis affected by available nutrient and regulated by autoinducer molecules through quorum sensingmechanism

scholarly journals SECONDARY METABOLITES WITH ANTIBIOTIC ACTIVITY OF MARINE ACTINOBACTERIA

2021 ◽  
pp. 28-43
Author(s):  
K. S. Potapenko ◽  
N. V. Korotaieva ◽  
V. О. Ivanytsia
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Bioactive Compounds ◽  
Current Literature ◽  
Antibiotic Activity ◽  
Growth Hormones ◽  
Biologically Active ◽  
Marine Actinobacteria ◽  
Biotechnological Potential ◽  
Plant Growth Hormones

Marine actinobacteria are active producers and an unused rich source of various biologically active secondary metabolites, such as antibiotics, antitumor, antiviral and antiinflammatory compounds, biopesticides, plant growth hormones, pigments, enzymes, enzyme inhibitors.In this review describes data from current literature sources for the period from 2017 to 2021 about various bioactive compounds that produce marine actinobacteria, their antibiotic activity and biotechnological potential, the main groups of secondary metabolites and their producers.

scholarly journals Secondary metabolites of a marine-derived Penicillium ochrochloron

2021 ◽  
Vol 13 (3) ◽  
pp. 11020
Author(s):  
Peter M. EZE ◽  
Ying GAO ◽  
Yang LIU ◽  
Lasse Van GEELEN ◽  
Chika P. EJIKEUGWU ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Biological Properties ◽  
Industrial Applications ◽  
Biologically Active ◽  
Fungal Isolation ◽  
The North ◽  
Wide Range ◽  
Extremophilic Fungi ◽  
Fungal Secondary Metabolites

Extremophilic fungi have received considerable attention recently as new promising sources of biologically active compounds with potential pharmaceutical applications. This study investigated the secondary metabolites of a marine-derived Penicillium ochrochloron isolated from underwater sea sand collected from the North Sea in St. Peter-Ording, Germany. Standard techniques were used for fungal isolation, taxonomic identification, fermentation, extraction, and isolation of fungal secondary metabolites. Chromatographic separation and spectroscopic analyses of the fungal secondary metabolites yielded eight compounds: talumarin A (1), aspergillumarin A (2), andrastin A (3), clavatol (4), 3-acetylphenol (5), methyl 2,5-dihydro-4-hydroxy-5-oxo-3-phenyl-2-furanpropanoate (6), emodin (7) and 2-chloroemodin (8). After co-cultivation with Bacillus subtilis, the fungus was induced to express (-)-striatisporolide A (9). Compound 1 was evaluated for antibacterial activity against Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and M. tuberculosis, as well as cytotoxicity against THP-1 cells. The compound, however, was not cytotoxic to THP-1 cells and had no antibacterial activity against the microorganisms tested. The compounds isolated from P. ochrochloron in this study are well-known compounds with a wide range of beneficial biological properties that can be explored for pharmaceutical, agricultural, or industrial applications. This study highlights the bioprospecting potential of marine fungi and confirms co-cultivation as a useful strategy for the discovery of new natural products.

scholarly journals THE STUDY OF THE DOMINANT GROUPS OF BIOLOGICALLY ACTIVE SUBSTANCES AND BIOELEMENTS IN SOME PLANTS OF THE FAMILY ROSACEAE

2017 ◽  
pp. 145-151
Author(s):  
Ефим (Efim) Авраамович (Avraamovich) Краснов (Krasnov) ◽  
Елена (Elena) Евгеньевна (Evgen'evna) Савельева (Savelyeva) ◽  
Надежда (Nadezhda) Кирилловна (Kirillovna) Рыжакова (Ryzhakova) ◽  
Ярослав (Yaroslav) Евгеньевич (Evgen'evich) Решетов (Reshetov) ◽  
Альбина (Al'bina) Равильевна (Ravil'evna) Гатауллина (Gataullina)
Keyword(s):  
Atomic Emission ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Isolation And Identification ◽  
Leading Role ◽  
Aerial Parts ◽  
Potentilla Anserina ◽  
Rosaceae Family ◽  
Innovative Drugs ◽  
Active Substances

To determine the content of the dominant groups of biologically active substances (tannins, polysaccharides, flavonoids) and bioelements in aerial parts of ten species of the Rosaceae family, which grows in Siberia: Potentilla anserina L., P. longifolia Willd., P. canescens Besser, P. bifurca L., P. tergemina Sojak, P. argentea L., P. goldbachii Rupr., P. sericea Dulac., Agrimonia pilosa Ledeb., Filipendula ulmaria (L.) Maxim. To determine the concentrations of essential and conditionally essential trace elements defined by the method of atomic emission spectroscopy. First identified plants of the genus Potentilla (P. canescens, P. tergemina) and Filipendula (F. ulmaria), accumulate significant quantities of lithium – 14,6, and 11,0 13,3 mg/kg, respectively, making them promising for further studies on the isolation and identification of active substances with the aim of creating innovative drugs. Investigated the content of heavy metals (Pb, Hg, As, Cd), the values of which meets regulatory requirements. Useful properties of the examined species family Rosaceae are determined by the content of biologically active substances (flavonoids, polysaccharides, tannins) and the complex of macro - and micronutrients. It is revealed that a dominant position in the spectrum belongs to the macronutrients potassium, calcium and magnesium (4,300 to 27,000 mg/kg). Among micronutrients the leading role is copper, followed by iron, silicon and manganese having important biological value, in particular, involved in the process of hematopoiesis.

Sign in / Sign up

Export Citation Format

Share Document