Chemical Composition, Structure, and Metal Binding Properties

Author(s):  
E. Michael Perdue
Author(s):  
Martin Poenie ◽  
Akwasi Minta ◽  
Charles Vorndran

The use of fura-2 as an intracellular calcium indicator is complicated by problems of rapid dye leakage and intracellular compartmentalization which is due to a probenecid sensitive anion transporter. In addition there is increasing evidence for localized microdomains of high calcium signals which may not be faithfully reported by fura-2.We have developed a new family of fura-2 analogs aimed at addressing some of these problems. These new indicators are based on a modified bapta which can be readily derivatized to produce fura-2 analogs with a variety of new properties. The modifications do not affect the chromophore and have little impact on the spectral and metal binding properties of the indicator. One of these new derivatives known as FPE3 is a zwitterionic analog of fura-2 that can be loaded into cells as an acetoxymethyl ester and whose retention in cells is much improved. The improved retention of FPE3 is important for both cuvettebased measurements of cell suspensions and for calcium imaging.


2020 ◽  
Vol 236 ◽  
pp. 116000 ◽  
Author(s):  
Biljana M. Pejić ◽  
Ana D. Kramar ◽  
Bratislav M. Obradović ◽  
Milorad M. Kuraica ◽  
Andrijana A. Žekić ◽  
...  

2001 ◽  
Vol 35 (12) ◽  
pp. 2512-2517 ◽  
Author(s):  
Iso Christl ◽  
Chris J. Milne ◽  
David G. Kinniburgh ◽  
Ruben Kretzschmar

2003 ◽  
Vol 5 (12) ◽  
pp. 2051-2054 ◽  
Author(s):  
Matthew A. Clark ◽  
Kathryn Duffy ◽  
Jyoti Tibrewala ◽  
Stephen J. Lippard

1983 ◽  
Vol 77 ◽  
pp. L63-L64 ◽  
Author(s):  
Bridget A. Sayer ◽  
Joseph P. Michael ◽  
Robert D. Hancock

Genes ◽  
2016 ◽  
Vol 7 (12) ◽  
pp. 106 ◽  
Author(s):  
Yu Pan ◽  
Yanglu Pan ◽  
Junpeng Zhai ◽  
Yan Xiong ◽  
Jinhua Li ◽  
...  

1997 ◽  
Vol 272 (52) ◽  
pp. 33279-33282 ◽  
Author(s):  
Michael DiDonato ◽  
Suree Narindrasorasak ◽  
John R. Forbes ◽  
Diane W. Cox ◽  
Bibudhendra Sarkar

2021 ◽  
Vol 316 ◽  
pp. 1019-1024
Author(s):  
O. A. Ignatova ◽  
A. A. Dyatchina

The paper presents the studies’ results of chemical composition, structure, and physico-mechanical properties of high-calcium ashes from the Kansk-Achinsk coals (2017-2019 selection). It was found that ash has a complex poly-mineral composition and contains hydraulically active minerals and oxides of СаОfr, β-C2S, CA, C3A, C4AF, C2F, CaSO4. According to the content of CaOfr, MgO does not meet standards’ requirements. The uniformity of the volume change is maintained by the composition with 50% of cement. The structure and hardening kinetics of ash and ash-cement stone compositions, obtained from the test of normal density, were analyzed. It was established that the hardening of compositions with ash from the Kansk-Achinsk coals was largely influenced by ash minerals. An equivalent amount of cement in composite binders cannot be replaced. In order to obtain a positive effect, compositions with ash instead cement of no more than 30% and a part of fine aggregate, without exceeding the ratio of ash: cement = 1: 1, should be used.


Author(s):  
Xiao-Hua Cai ◽  
Jagat Adhiya ◽  
Samuel Traina ◽  
Richard Sayre

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